An insight into the cytotoxic activity of phytol at in vitro conditions
Abstract
The cytotoxicity of the diterpene alcohol, phytol, was evaluated by using the MTT assay in vitro against seven tumour cells and one normal cell of human origin. The compound tested induced concentration-dependent cytotoxic response in all cell lines, demonstrating to be most and least effective against the breast adenocarcinoma MCF-7 and the prostate adenocarcinoma PC-3 cells, respectively (IC50 8.79 +/- 0.41 mu M and 77.85 +/- 1.93 mu M). The IC50 values towards the other five tumours (HeLa, HT-29, A-549, Hs294T and MDA-MB-231) ranged from 15.51 to 69.67 mu M. However, mild toxicity was detected against the foetal lung fibroblast MRC-5 cells at the concentrations used (IC50 124.84 +/- 1.59 mu M). According to the experimental data obtained, this cost-effective natural product widely present in the biosphere may inspire the development of new drug-like substances with improved cytotoxic activity on breast cancer.
Keywords:
MTT assay / MCF-7 cells / diterpene alcoholSource:
Natural Product Research, 2014, 28, 22, 2053-2056Publisher:
- Taylor & Francis Ltd, Abingdon
Funding / projects:
- Synthesis and biological testing of new mimics or derivatives of selected cytotoxic lactones, antitumor agent tiazofurin and natural naphthenic acids (RS-172006)
- Natural products of wild, cultivated and edible plants: structure and bioactivity determination (RS-172053)
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-173017)
DOI: 10.1080/14786419.2014.921686
ISSN: 1478-6419
PubMed: 24896297
WoS: 000342325200017
Scopus: 2-s2.0-84908483702
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Pejin, Boris AU - Kojić, Vesna AU - Bogdanović, Gordana PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/771 AB - The cytotoxicity of the diterpene alcohol, phytol, was evaluated by using the MTT assay in vitro against seven tumour cells and one normal cell of human origin. The compound tested induced concentration-dependent cytotoxic response in all cell lines, demonstrating to be most and least effective against the breast adenocarcinoma MCF-7 and the prostate adenocarcinoma PC-3 cells, respectively (IC50 8.79 +/- 0.41 mu M and 77.85 +/- 1.93 mu M). The IC50 values towards the other five tumours (HeLa, HT-29, A-549, Hs294T and MDA-MB-231) ranged from 15.51 to 69.67 mu M. However, mild toxicity was detected against the foetal lung fibroblast MRC-5 cells at the concentrations used (IC50 124.84 +/- 1.59 mu M). According to the experimental data obtained, this cost-effective natural product widely present in the biosphere may inspire the development of new drug-like substances with improved cytotoxic activity on breast cancer. PB - Taylor & Francis Ltd, Abingdon T2 - Natural Product Research T1 - An insight into the cytotoxic activity of phytol at in vitro conditions EP - 2056 IS - 22 SP - 2053 VL - 28 DO - 10.1080/14786419.2014.921686 ER -
@article{ author = "Pejin, Boris and Kojić, Vesna and Bogdanović, Gordana", year = "2014", abstract = "The cytotoxicity of the diterpene alcohol, phytol, was evaluated by using the MTT assay in vitro against seven tumour cells and one normal cell of human origin. The compound tested induced concentration-dependent cytotoxic response in all cell lines, demonstrating to be most and least effective against the breast adenocarcinoma MCF-7 and the prostate adenocarcinoma PC-3 cells, respectively (IC50 8.79 +/- 0.41 mu M and 77.85 +/- 1.93 mu M). The IC50 values towards the other five tumours (HeLa, HT-29, A-549, Hs294T and MDA-MB-231) ranged from 15.51 to 69.67 mu M. However, mild toxicity was detected against the foetal lung fibroblast MRC-5 cells at the concentrations used (IC50 124.84 +/- 1.59 mu M). According to the experimental data obtained, this cost-effective natural product widely present in the biosphere may inspire the development of new drug-like substances with improved cytotoxic activity on breast cancer.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Natural Product Research", title = "An insight into the cytotoxic activity of phytol at in vitro conditions", pages = "2056-2053", number = "22", volume = "28", doi = "10.1080/14786419.2014.921686" }
Pejin, B., Kojić, V.,& Bogdanović, G.. (2014). An insight into the cytotoxic activity of phytol at in vitro conditions. in Natural Product Research Taylor & Francis Ltd, Abingdon., 28(22), 2053-2056. https://doi.org/10.1080/14786419.2014.921686
Pejin B, Kojić V, Bogdanović G. An insight into the cytotoxic activity of phytol at in vitro conditions. in Natural Product Research. 2014;28(22):2053-2056. doi:10.1080/14786419.2014.921686 .
Pejin, Boris, Kojić, Vesna, Bogdanović, Gordana, "An insight into the cytotoxic activity of phytol at in vitro conditions" in Natural Product Research, 28, no. 22 (2014):2053-2056, https://doi.org/10.1080/14786419.2014.921686 . .