In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches
Апстракт
Fructooligosaccharides (FOS) are bioactive oligosaccharide fructans with beneficial health effects. Anti-hydroxyl radical activity is an important factor in the description of antioxidant capacity of any natural product. The aim of this study was to estimate in vitro anti-hydroxyl radical activity of the FOS 1-kestose and nystose by electron paramagnetic resonance spectroscopy (EPR) and fluorescence spectroscopy (FS) followed by a theoretical approach based on quantum chemistry calculations. A significant anti-hydroxyl radical potential of both compounds was observed (72% and 78% by EPR and 69% and 74% by FS, respectively), indicating the nystose to be a more active natural product. In addition, the computational results have confirmed that nystose follows the same pattern previously shown for 1-kestose, that is, that carbohydrates can react with hydroxyl radical. It is well known that FOS belong to cardioprotective nutraceuticals, so the study may be of some interest to research in he...art disease.
Кључне речи:
nystose / hydroxyl radical / heart medicine / fluorescence spectroscopy / electron paramagnetic resonance spectroscopy / dietary food supplements / computational chemistry / 1-kestoseИзвор:
International Journal of Food Science and Technology, 2014, 49, 6, 1500-1505Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-MESTD-Basic Research (BR or ON)-173040)
- Биомаркери у неуродегенеративним и малигним процесима (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41005)
- Синтеза, процесирање и карактеризација наноструктурних материјала за примену у области енергије, механичког инжењерства, заштите животне стредине и биомедицине (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45012)
DOI: 10.1111/ijfs.12445
ISSN: 0950-5423
WoS: 000335940600009
Scopus: 2-s2.0-84900507671
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Pejin, Boris AU - Savić, Aleksandar G AU - Petković, Milena M AU - Radotić, Ksenija AU - Mojović, Miloš PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/752 AB - Fructooligosaccharides (FOS) are bioactive oligosaccharide fructans with beneficial health effects. Anti-hydroxyl radical activity is an important factor in the description of antioxidant capacity of any natural product. The aim of this study was to estimate in vitro anti-hydroxyl radical activity of the FOS 1-kestose and nystose by electron paramagnetic resonance spectroscopy (EPR) and fluorescence spectroscopy (FS) followed by a theoretical approach based on quantum chemistry calculations. A significant anti-hydroxyl radical potential of both compounds was observed (72% and 78% by EPR and 69% and 74% by FS, respectively), indicating the nystose to be a more active natural product. In addition, the computational results have confirmed that nystose follows the same pattern previously shown for 1-kestose, that is, that carbohydrates can react with hydroxyl radical. It is well known that FOS belong to cardioprotective nutraceuticals, so the study may be of some interest to research in heart disease. PB - Wiley, Hoboken T2 - International Journal of Food Science and Technology T1 - In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches EP - 1505 IS - 6 SP - 1500 VL - 49 DO - 10.1111/ijfs.12445 ER -
@article{ author = "Pejin, Boris and Savić, Aleksandar G and Petković, Milena M and Radotić, Ksenija and Mojović, Miloš", year = "2014", abstract = "Fructooligosaccharides (FOS) are bioactive oligosaccharide fructans with beneficial health effects. Anti-hydroxyl radical activity is an important factor in the description of antioxidant capacity of any natural product. The aim of this study was to estimate in vitro anti-hydroxyl radical activity of the FOS 1-kestose and nystose by electron paramagnetic resonance spectroscopy (EPR) and fluorescence spectroscopy (FS) followed by a theoretical approach based on quantum chemistry calculations. A significant anti-hydroxyl radical potential of both compounds was observed (72% and 78% by EPR and 69% and 74% by FS, respectively), indicating the nystose to be a more active natural product. In addition, the computational results have confirmed that nystose follows the same pattern previously shown for 1-kestose, that is, that carbohydrates can react with hydroxyl radical. It is well known that FOS belong to cardioprotective nutraceuticals, so the study may be of some interest to research in heart disease.", publisher = "Wiley, Hoboken", journal = "International Journal of Food Science and Technology", title = "In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches", pages = "1505-1500", number = "6", volume = "49", doi = "10.1111/ijfs.12445" }
Pejin, B., Savić, A. G., Petković, M. M., Radotić, K.,& Mojović, M.. (2014). In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches. in International Journal of Food Science and Technology Wiley, Hoboken., 49(6), 1500-1505. https://doi.org/10.1111/ijfs.12445
Pejin B, Savić AG, Petković MM, Radotić K, Mojović M. In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches. in International Journal of Food Science and Technology. 2014;49(6):1500-1505. doi:10.1111/ijfs.12445 .
Pejin, Boris, Savić, Aleksandar G, Petković, Milena M, Radotić, Ksenija, Mojović, Miloš, "In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches" in International Journal of Food Science and Technology, 49, no. 6 (2014):1500-1505, https://doi.org/10.1111/ijfs.12445 . .