Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones
Samo za registrovane korisnike
2013
Autori
Ajdzanović, Vladimir ZMojic, Marija
Maksimović-Ivanić, Danijela
Bulatović, Mirna Z
Mijatović, Sanja
Milošević, Verica Lj.
Spasojević, Ivan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Soy isoflavones represent hopeful unconventional remedies in the therapy of prostate cancer. The aim of our study was to determine the effects of genistein and daidzein on the parameters that reflect metastatic potential, membrane fluidity, invasiveness and dynamic phenotype in Matrigel of LNCaP and PC-3 prostate cancer cells. Cell viability tests, using a wide range of concentrations of soy isoflavones (6-75 mu g/ml for 72 h), were conducted to determine their IC50 concentrations. Electron paramagnetic resonance investigations of prostate cancer cell membrane fluidity were performed at IC50 concentrations of genistein and daidzein (12.5 and 25 mu g/ml, respectively, for 10 min). Genistein provoked significant increases in the membrane order parameter (which is reciprocally proportional to membrane fluidity) of 0.722 +/- A 0.006 (LNCaP), 0.753 +/- A 0.010 (LNCaP + genistein), 0.723 +/- A 0.007 (PC-3) and 0.741 +/- A 0.004 (PC-3 + genistein); however, no such effects were observed for d...aidzein. While both genistein and daidzein reduced the proliferation of prostate cancer cells at their respective IC50 concentrations, during the 72 h of incubation only genistein provoked effects on the dynamic phenotype and decreased invasiveness. The effect was more evident in PC-3 cells compared to LNCaP cells. Our results imply that (1) invasive activity is at least partially dependent on membrane fluidity, (2) genistein may exert its antimetastatic effects by changing the mechanical properties of prostate cancer cells and (3) daidzein should be applied at higher concentrations than genistein in order to achieve pharmacological effects.
Ključne reči:
Prostate cancer / Metastasis / Membrane fluidity / Invasiveness / Genistein / DaidzeinIzvor:
Journal of Membrane Biology, 2013, 246, 4, 307-314Izdavač:
- Springer, New York
Finansiranje / projekti:
- Odgovor neuroendokrinog sistema pacova na odabrane biljne ekstrakte, fitoestrogene, steroidne i peptidne hormone (RS-MESTD-Basic Research (BR or ON)-173009)
- Molekularni mehanizmi fiziološke i farmakološke kontrole inflamacije i kancera (RS-MESTD-Basic Research (BR or ON)-173013)
- Molekularni mehanizmi redoks signalinga u homeostazi, adaptaciji i patologiji (RS-MESTD-Basic Research (BR or ON)-173014)
DOI: 10.1007/s00232-013-9531-1
ISSN: 0022-2631
PubMed: 23417033
WoS: 000317610900005
Scopus: 2-s2.0-84876412215
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Ajdzanović, Vladimir Z AU - Mojic, Marija AU - Maksimović-Ivanić, Danijela AU - Bulatović, Mirna Z AU - Mijatović, Sanja AU - Milošević, Verica Lj. AU - Spasojević, Ivan PY - 2013 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/729 AB - Soy isoflavones represent hopeful unconventional remedies in the therapy of prostate cancer. The aim of our study was to determine the effects of genistein and daidzein on the parameters that reflect metastatic potential, membrane fluidity, invasiveness and dynamic phenotype in Matrigel of LNCaP and PC-3 prostate cancer cells. Cell viability tests, using a wide range of concentrations of soy isoflavones (6-75 mu g/ml for 72 h), were conducted to determine their IC50 concentrations. Electron paramagnetic resonance investigations of prostate cancer cell membrane fluidity were performed at IC50 concentrations of genistein and daidzein (12.5 and 25 mu g/ml, respectively, for 10 min). Genistein provoked significant increases in the membrane order parameter (which is reciprocally proportional to membrane fluidity) of 0.722 +/- A 0.006 (LNCaP), 0.753 +/- A 0.010 (LNCaP + genistein), 0.723 +/- A 0.007 (PC-3) and 0.741 +/- A 0.004 (PC-3 + genistein); however, no such effects were observed for daidzein. While both genistein and daidzein reduced the proliferation of prostate cancer cells at their respective IC50 concentrations, during the 72 h of incubation only genistein provoked effects on the dynamic phenotype and decreased invasiveness. The effect was more evident in PC-3 cells compared to LNCaP cells. Our results imply that (1) invasive activity is at least partially dependent on membrane fluidity, (2) genistein may exert its antimetastatic effects by changing the mechanical properties of prostate cancer cells and (3) daidzein should be applied at higher concentrations than genistein in order to achieve pharmacological effects. PB - Springer, New York T2 - Journal of Membrane Biology T1 - Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones EP - 314 IS - 4 SP - 307 VL - 246 DO - 10.1007/s00232-013-9531-1 ER -
@article{ author = "Ajdzanović, Vladimir Z and Mojic, Marija and Maksimović-Ivanić, Danijela and Bulatović, Mirna Z and Mijatović, Sanja and Milošević, Verica Lj. and Spasojević, Ivan", year = "2013", abstract = "Soy isoflavones represent hopeful unconventional remedies in the therapy of prostate cancer. The aim of our study was to determine the effects of genistein and daidzein on the parameters that reflect metastatic potential, membrane fluidity, invasiveness and dynamic phenotype in Matrigel of LNCaP and PC-3 prostate cancer cells. Cell viability tests, using a wide range of concentrations of soy isoflavones (6-75 mu g/ml for 72 h), were conducted to determine their IC50 concentrations. Electron paramagnetic resonance investigations of prostate cancer cell membrane fluidity were performed at IC50 concentrations of genistein and daidzein (12.5 and 25 mu g/ml, respectively, for 10 min). Genistein provoked significant increases in the membrane order parameter (which is reciprocally proportional to membrane fluidity) of 0.722 +/- A 0.006 (LNCaP), 0.753 +/- A 0.010 (LNCaP + genistein), 0.723 +/- A 0.007 (PC-3) and 0.741 +/- A 0.004 (PC-3 + genistein); however, no such effects were observed for daidzein. While both genistein and daidzein reduced the proliferation of prostate cancer cells at their respective IC50 concentrations, during the 72 h of incubation only genistein provoked effects on the dynamic phenotype and decreased invasiveness. The effect was more evident in PC-3 cells compared to LNCaP cells. Our results imply that (1) invasive activity is at least partially dependent on membrane fluidity, (2) genistein may exert its antimetastatic effects by changing the mechanical properties of prostate cancer cells and (3) daidzein should be applied at higher concentrations than genistein in order to achieve pharmacological effects.", publisher = "Springer, New York", journal = "Journal of Membrane Biology", title = "Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones", pages = "314-307", number = "4", volume = "246", doi = "10.1007/s00232-013-9531-1" }
Ajdzanović, V. Z., Mojic, M., Maksimović-Ivanić, D., Bulatović, M. Z., Mijatović, S., Milošević, V. Lj.,& Spasojević, I.. (2013). Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones. in Journal of Membrane Biology Springer, New York., 246(4), 307-314. https://doi.org/10.1007/s00232-013-9531-1
Ajdzanović VZ, Mojic M, Maksimović-Ivanić D, Bulatović MZ, Mijatović S, Milošević VL, Spasojević I. Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones. in Journal of Membrane Biology. 2013;246(4):307-314. doi:10.1007/s00232-013-9531-1 .
Ajdzanović, Vladimir Z, Mojic, Marija, Maksimović-Ivanić, Danijela, Bulatović, Mirna Z, Mijatović, Sanja, Milošević, Verica Lj., Spasojević, Ivan, "Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones" in Journal of Membrane Biology, 246, no. 4 (2013):307-314, https://doi.org/10.1007/s00232-013-9531-1 . .