A universal fluid cell for the imaging of biological specimens in the atomic force microscope
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Authors
Kasas, SandorRadotić, Ksenija
Longo, Giovanni
Saha, Bashkar
Alonso-Sarduy, Livan
Dietler, Giovanni
Roduit, Charles
Article (Published version)
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Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM imaging. An important component of the set-up is the imaging chamber, whose design determines the nature of the experiments that can be conducted. Many different imaging chamber designs are available, usually designed to optimize a single parameter, such as the dimensions of the substrate or the volume of fluid that can be used throughout the experiment. In this report, we present a universal fluid cell, which simultaneously optimizes all of the parameters that are important for the imaging of biological specimens in the AFM. This novel imaging chamber has been successfully tested using mammalian, plant, and microbial cells. Microsc. Res. Tech. 76:357363, 2013.
Keywords:
mechanical properties / macrophage / fluid cell / E / coli / Arabidopsis thaliana / AFMSource:
Microscopy Research and Technique, 2013, 76, 4, 357-363Publisher:
- Wiley-Blackwell, Hoboken
Funding / projects:
- Indo Swiss Joint Research Program (ISJRP) [122941]
- Swiss National Science FoundationSwiss National Science Foundation (SNSF)European Commission [CR 32I3-130676]
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-MESTD-Basic Research (BR or ON)-173017)
DOI: 10.1002/jemt.22174
ISSN: 1059-910X
PubMed: 23390022
WoS: 000316699400004
Scopus: 2-s2.0-84875621860
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Kasas, Sandor AU - Radotić, Ksenija AU - Longo, Giovanni AU - Saha, Bashkar AU - Alonso-Sarduy, Livan AU - Dietler, Giovanni AU - Roduit, Charles PY - 2013 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/723 AB - Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM imaging. An important component of the set-up is the imaging chamber, whose design determines the nature of the experiments that can be conducted. Many different imaging chamber designs are available, usually designed to optimize a single parameter, such as the dimensions of the substrate or the volume of fluid that can be used throughout the experiment. In this report, we present a universal fluid cell, which simultaneously optimizes all of the parameters that are important for the imaging of biological specimens in the AFM. This novel imaging chamber has been successfully tested using mammalian, plant, and microbial cells. Microsc. Res. Tech. 76:357363, 2013. PB - Wiley-Blackwell, Hoboken T2 - Microscopy Research and Technique T1 - A universal fluid cell for the imaging of biological specimens in the atomic force microscope EP - 363 IS - 4 SP - 357 VL - 76 DO - 10.1002/jemt.22174 ER -
@article{ author = "Kasas, Sandor and Radotić, Ksenija and Longo, Giovanni and Saha, Bashkar and Alonso-Sarduy, Livan and Dietler, Giovanni and Roduit, Charles", year = "2013", abstract = "Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM imaging. An important component of the set-up is the imaging chamber, whose design determines the nature of the experiments that can be conducted. Many different imaging chamber designs are available, usually designed to optimize a single parameter, such as the dimensions of the substrate or the volume of fluid that can be used throughout the experiment. In this report, we present a universal fluid cell, which simultaneously optimizes all of the parameters that are important for the imaging of biological specimens in the AFM. This novel imaging chamber has been successfully tested using mammalian, plant, and microbial cells. Microsc. Res. Tech. 76:357363, 2013.", publisher = "Wiley-Blackwell, Hoboken", journal = "Microscopy Research and Technique", title = "A universal fluid cell for the imaging of biological specimens in the atomic force microscope", pages = "363-357", number = "4", volume = "76", doi = "10.1002/jemt.22174" }
Kasas, S., Radotić, K., Longo, G., Saha, B., Alonso-Sarduy, L., Dietler, G.,& Roduit, C.. (2013). A universal fluid cell for the imaging of biological specimens in the atomic force microscope. in Microscopy Research and Technique Wiley-Blackwell, Hoboken., 76(4), 357-363. https://doi.org/10.1002/jemt.22174
Kasas S, Radotić K, Longo G, Saha B, Alonso-Sarduy L, Dietler G, Roduit C. A universal fluid cell for the imaging of biological specimens in the atomic force microscope. in Microscopy Research and Technique. 2013;76(4):357-363. doi:10.1002/jemt.22174 .
Kasas, Sandor, Radotić, Ksenija, Longo, Giovanni, Saha, Bashkar, Alonso-Sarduy, Livan, Dietler, Giovanni, Roduit, Charles, "A universal fluid cell for the imaging of biological specimens in the atomic force microscope" in Microscopy Research and Technique, 76, no. 4 (2013):357-363, https://doi.org/10.1002/jemt.22174 . .