UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid
Nema prikaza
Autori
Bogdanović Pristov, JelenaVeljović-Jovanović, Sonja
Mitrović, Aleksandra Lj.
Spasojević, Ivan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
We examined the redox effects of UV irradiation on cell wall isolates from Pisum sativum leaves, and polygalacturonic and galacturonic acid, in the presence of hydrogen peroxide. For this purpose, electron paramagnetic resonance spectroscopy and two spin-traps (DEPMPO and BMPO), capable of differentiating between various free radicals, were applied. Systems were exposed to UV-B (maximum emission at 312nm) and UV-A (352nm) for 10min (6Jm(-2)s(-1)). Cell wall isolates exposed to UV in the presence of hydrogen peroxide, produced hydroxyl radical, carbon dioxide radical and superoxide. The production of superoxide was observed for cell wall isolates, polygalacturonic acid (in the presence and in the absence of calcium) and galacturonic acid, and it was diminished upon superoxide dismutase supplementation. The production is at least partially based on the reaction of hydroxyl radicals with (poly)galacturonic acid having carbon dioxide radicals as a products. Acting as a strong reducing agen...t, carbon dioxide radical reacts with molecular oxygen to produce superoxide. The results presented here shed a new light on: (1) the redox-modulating role of cell wall; (2) the production of superoxide in the extracellular compartment; (3) the mechanisms involved in translating UV stress into molecular signaling and (4) some other UV-related phenomena in plants, such as CO2 emission.
Ključne reči:
UV-B / Plant cell walls / reactive oxygen species / galacturonic acid / EPRIzvor:
Physiologia Plantarum, 2013, 148, 4, 574-581Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Ispitivanja odnosa struktura-funkcija u ćelijskom zidu biljaka i izmene strukture zida enzimskim inženjeringom (RS-MESTD-Basic Research (BR or ON)-173017)
- Modifikacije antioksidativnog metabolizma biljaka sa ciljem povećanja tolerancije na abiotski stres i identifikacija novih biomarkera sa primenom u remedijaciji i monitoringu degradiranih staništa (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
DOI: 10.1111/ppl.12001
ISSN: 0031-9317
PubMed: 23163764
WoS: 000321697300011
Scopus: 2-s2.0-84880329322
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Bogdanović Pristov, Jelena AU - Veljović-Jovanović, Sonja AU - Mitrović, Aleksandra Lj. AU - Spasojević, Ivan PY - 2013 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/673 AB - We examined the redox effects of UV irradiation on cell wall isolates from Pisum sativum leaves, and polygalacturonic and galacturonic acid, in the presence of hydrogen peroxide. For this purpose, electron paramagnetic resonance spectroscopy and two spin-traps (DEPMPO and BMPO), capable of differentiating between various free radicals, were applied. Systems were exposed to UV-B (maximum emission at 312nm) and UV-A (352nm) for 10min (6Jm(-2)s(-1)). Cell wall isolates exposed to UV in the presence of hydrogen peroxide, produced hydroxyl radical, carbon dioxide radical and superoxide. The production of superoxide was observed for cell wall isolates, polygalacturonic acid (in the presence and in the absence of calcium) and galacturonic acid, and it was diminished upon superoxide dismutase supplementation. The production is at least partially based on the reaction of hydroxyl radicals with (poly)galacturonic acid having carbon dioxide radicals as a products. Acting as a strong reducing agent, carbon dioxide radical reacts with molecular oxygen to produce superoxide. The results presented here shed a new light on: (1) the redox-modulating role of cell wall; (2) the production of superoxide in the extracellular compartment; (3) the mechanisms involved in translating UV stress into molecular signaling and (4) some other UV-related phenomena in plants, such as CO2 emission. PB - Wiley, Hoboken T2 - Physiologia Plantarum T1 - UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid EP - 581 IS - 4 SP - 574 VL - 148 DO - 10.1111/ppl.12001 ER -
@article{ author = "Bogdanović Pristov, Jelena and Veljović-Jovanović, Sonja and Mitrović, Aleksandra Lj. and Spasojević, Ivan", year = "2013", abstract = "We examined the redox effects of UV irradiation on cell wall isolates from Pisum sativum leaves, and polygalacturonic and galacturonic acid, in the presence of hydrogen peroxide. For this purpose, electron paramagnetic resonance spectroscopy and two spin-traps (DEPMPO and BMPO), capable of differentiating between various free radicals, were applied. Systems were exposed to UV-B (maximum emission at 312nm) and UV-A (352nm) for 10min (6Jm(-2)s(-1)). Cell wall isolates exposed to UV in the presence of hydrogen peroxide, produced hydroxyl radical, carbon dioxide radical and superoxide. The production of superoxide was observed for cell wall isolates, polygalacturonic acid (in the presence and in the absence of calcium) and galacturonic acid, and it was diminished upon superoxide dismutase supplementation. The production is at least partially based on the reaction of hydroxyl radicals with (poly)galacturonic acid having carbon dioxide radicals as a products. Acting as a strong reducing agent, carbon dioxide radical reacts with molecular oxygen to produce superoxide. The results presented here shed a new light on: (1) the redox-modulating role of cell wall; (2) the production of superoxide in the extracellular compartment; (3) the mechanisms involved in translating UV stress into molecular signaling and (4) some other UV-related phenomena in plants, such as CO2 emission.", publisher = "Wiley, Hoboken", journal = "Physiologia Plantarum", title = "UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid", pages = "581-574", number = "4", volume = "148", doi = "10.1111/ppl.12001" }
Bogdanović Pristov, J., Veljović-Jovanović, S., Mitrović, A. Lj.,& Spasojević, I.. (2013). UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid. in Physiologia Plantarum Wiley, Hoboken., 148(4), 574-581. https://doi.org/10.1111/ppl.12001
Bogdanović Pristov J, Veljović-Jovanović S, Mitrović AL, Spasojević I. UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid. in Physiologia Plantarum. 2013;148(4):574-581. doi:10.1111/ppl.12001 .
Bogdanović Pristov, Jelena, Veljović-Jovanović, Sonja, Mitrović, Aleksandra Lj., Spasojević, Ivan, "UV-irradiation provokes generation of superoxide on cell wall polygalacturonic acid" in Physiologia Plantarum, 148, no. 4 (2013):574-581, https://doi.org/10.1111/ppl.12001 . .