Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
Samo za registrovane korisnike
2012
Autori
Mitic, Nevena VDmitrović, Slavica
Đorđević, Mirka
Zdravkovic-Korac, Snežana R
Nikolic, Radomirka R
Raspor, Martin
Đorđević, Tatjana
Maksimović, Vuk
Živković, Suzana
Krstić-Milošević, Dijana B.
Stanisic, Mariana
Ninković, Slavica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition co...mpared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.
Ključne reči:
Rol genes / Phenolic acids / Hairy roots / Genetic transformation / Chenopodium murale / Allelopathy / Agrobacterium rhizogenesIzvor:
Journal of Plant Physiology, 2012, 169, 12, 1203-1211Izdavač:
- Elsevier Gmbh, Munich
Finansiranje / projekti:
- Biotehnologija in vitro - gajene, lekovite i ugrožene biljne vrste (RS-173015)
- Fiziološka, hemijska i molekularna analiza diverziteta odabranih retkih i ugroženih biljnih vrsta u cilju ex situ zaštite i produkcije biološki aktivnih jedinjenja (RS-173024)
- Interakcije membrana sa unutarćelijskim i apoplastičnim prostorom: izučavanja bioenergetike i singalizacije koristeći biofizičke i biohemijske metode (RS-173040)
DOI: 10.1016/j.jplph.2012.04.009
ISSN: 0176-1617
PubMed: 22749286
WoS: 000310392900010
Scopus: 2-s2.0-84863880682
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Mitic, Nevena V AU - Dmitrović, Slavica AU - Đorđević, Mirka AU - Zdravkovic-Korac, Snežana R AU - Nikolic, Radomirka R AU - Raspor, Martin AU - Đorđević, Tatjana AU - Maksimović, Vuk AU - Živković, Suzana AU - Krstić-Milošević, Dijana B. AU - Stanisic, Mariana AU - Ninković, Slavica PY - 2012 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/599 AB - We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. PB - Elsevier Gmbh, Munich T2 - Journal of Plant Physiology T1 - Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays EP - 1211 IS - 12 SP - 1203 VL - 169 DO - 10.1016/j.jplph.2012.04.009 ER -
@article{ author = "Mitic, Nevena V and Dmitrović, Slavica and Đorđević, Mirka and Zdravkovic-Korac, Snežana R and Nikolic, Radomirka R and Raspor, Martin and Đorđević, Tatjana and Maksimović, Vuk and Živković, Suzana and Krstić-Milošević, Dijana B. and Stanisic, Mariana and Ninković, Slavica", year = "2012", abstract = "We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.", publisher = "Elsevier Gmbh, Munich", journal = "Journal of Plant Physiology", title = "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays", pages = "1211-1203", number = "12", volume = "169", doi = "10.1016/j.jplph.2012.04.009" }
Mitic, N. V., Dmitrović, S., Đorđević, M., Zdravkovic-Korac, S. R., Nikolic, R. R., Raspor, M., Đorđević, T., Maksimović, V., Živković, S., Krstić-Milošević, D. B., Stanisic, M.,& Ninković, S.. (2012). Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology Elsevier Gmbh, Munich., 169(12), 1203-1211. https://doi.org/10.1016/j.jplph.2012.04.009
Mitic NV, Dmitrović S, Đorđević M, Zdravkovic-Korac SR, Nikolic RR, Raspor M, Đorđević T, Maksimović V, Živković S, Krstić-Milošević DB, Stanisic M, Ninković S. Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology. 2012;169(12):1203-1211. doi:10.1016/j.jplph.2012.04.009 .
Mitic, Nevena V, Dmitrović, Slavica, Đorđević, Mirka, Zdravkovic-Korac, Snežana R, Nikolic, Radomirka R, Raspor, Martin, Đorđević, Tatjana, Maksimović, Vuk, Živković, Suzana, Krstić-Milošević, Dijana B., Stanisic, Mariana, Ninković, Slavica, "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays" in Journal of Plant Physiology, 169, no. 12 (2012):1203-1211, https://doi.org/10.1016/j.jplph.2012.04.009 . .