Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis
Samo za registrovane korisnike
2012
Autori
Calic, Dusica DDevrnja, Nina M
Milojević, Jelena D
Kostić, Igor
Janosević, Dusica A
Budimir, Snežana M
Zdravkovic-Korac, Snežana R
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos wa...s 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.
Ključne reči:
secondary somatic embryogenesis / microspore culture / anther culture / Aesculus hyppocastanum / abscisic acidIzvor:
Hortscience, 2012, 47, 12, 1741-1744Izdavač:
- Amer Soc Horticultural Science, Alexandria
Finansiranje / projekti:
- Biotehnologija in vitro - gajene, lekovite i ugrožene biljne vrste (RS-MESTD-Basic Research (BR or ON)-173015)
- Modifikacije antioksidativnog metabolizma biljaka sa ciljem povećanja tolerancije na abiotski stres i identifikacija novih biomarkera sa primenom u remedijaciji i monitoringu degradiranih staništa (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43010)
DOI: 10.21273/HORTSCI.47.12.1741
ISSN: 0018-5345
WoS: 000315708400013
Scopus: 2-s2.0-84871912351
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Calic, Dusica D AU - Devrnja, Nina M AU - Milojević, Jelena D AU - Kostić, Igor AU - Janosević, Dusica A AU - Budimir, Snežana M AU - Zdravkovic-Korac, Snežana R PY - 2012 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/583 AB - The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry. PB - Amer Soc Horticultural Science, Alexandria T2 - Hortscience T1 - Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis EP - 1744 IS - 12 SP - 1741 VL - 47 DO - 10.21273/HORTSCI.47.12.1741 ER -
@article{ author = "Calic, Dusica D and Devrnja, Nina M and Milojević, Jelena D and Kostić, Igor and Janosević, Dusica A and Budimir, Snežana M and Zdravkovic-Korac, Snežana R", year = "2012", abstract = "The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.", publisher = "Amer Soc Horticultural Science, Alexandria", journal = "Hortscience", title = "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis", pages = "1744-1741", number = "12", volume = "47", doi = "10.21273/HORTSCI.47.12.1741" }
Calic, D. D., Devrnja, N. M., Milojević, J. D., Kostić, I., Janosević, D. A., Budimir, S. M.,& Zdravkovic-Korac, S. R.. (2012). Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience Amer Soc Horticultural Science, Alexandria., 47(12), 1741-1744. https://doi.org/10.21273/HORTSCI.47.12.1741
Calic DD, Devrnja NM, Milojević JD, Kostić I, Janosević DA, Budimir SM, Zdravkovic-Korac SR. Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience. 2012;47(12):1741-1744. doi:10.21273/HORTSCI.47.12.1741 .
Calic, Dusica D, Devrnja, Nina M, Milojević, Jelena D, Kostić, Igor, Janosević, Dusica A, Budimir, Snežana M, Zdravkovic-Korac, Snežana R, "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis" in Hortscience, 47, no. 12 (2012):1741-1744, https://doi.org/10.21273/HORTSCI.47.12.1741 . .