Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study
Nema prikaza
Autori
Ajdzanović, Vladimir ZSpasojević, Ivan
Filipović, Branko R
Sosic-Jurjević, Branka T
Sekulic, Milka I
Milošević, Verica Lj.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p lt 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p lt 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice th...an genistein in some cardiovascular conditions.
Ključne reči:
spin probes / order parameter / membrane fluidity / genistein / erythrocytes / EPR / daidzeinIzvor:
Canadian Journal of Physiology and Pharmacology, 2010, 88, 4, 497-500Izdavač:
- Natl Research Council Canada-N R C Research Press, Ottawa
Finansiranje / projekti:
- Uticaj fitoestrogena, steroidnih i peptidnih hormona na ćelije neuroendokrinog sistema (RS-MESTD-MPN2006-2010-143007)
- Biofizička istraživanja membranskih procesa: interakcija membranskih receptora i kanala sa spoljašnjim faktorima i intracelularna regulacija (RS-MESTD-MPN2006-2010-143016)
DOI: 10.1139/Y10-020
ISSN: 0008-4212
PubMed: 20555419
WoS: 000279243700012
Scopus: 2-s2.0-77952068968
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Ajdzanović, Vladimir Z AU - Spasojević, Ivan AU - Filipović, Branko R AU - Sosic-Jurjević, Branka T AU - Sekulic, Milka I AU - Milošević, Verica Lj. PY - 2010 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/414 AB - The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p lt 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p lt 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions. PB - Natl Research Council Canada-N R C Research Press, Ottawa T2 - Canadian Journal of Physiology and Pharmacology T1 - Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study EP - 500 IS - 4 SP - 497 VL - 88 DO - 10.1139/Y10-020 ER -
@article{ author = "Ajdzanović, Vladimir Z and Spasojević, Ivan and Filipović, Branko R and Sosic-Jurjević, Branka T and Sekulic, Milka I and Milošević, Verica Lj.", year = "2010", abstract = "The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p lt 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p lt 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.", publisher = "Natl Research Council Canada-N R C Research Press, Ottawa", journal = "Canadian Journal of Physiology and Pharmacology", title = "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study", pages = "500-497", number = "4", volume = "88", doi = "10.1139/Y10-020" }
Ajdzanović, V. Z., Spasojević, I., Filipović, B. R., Sosic-Jurjević, B. T., Sekulic, M. I.,& Milošević, V. Lj.. (2010). Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology Natl Research Council Canada-N R C Research Press, Ottawa., 88(4), 497-500. https://doi.org/10.1139/Y10-020
Ajdzanović VZ, Spasojević I, Filipović BR, Sosic-Jurjević BT, Sekulic MI, Milošević VL. Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology. 2010;88(4):497-500. doi:10.1139/Y10-020 .
Ajdzanović, Vladimir Z, Spasojević, Ivan, Filipović, Branko R, Sosic-Jurjević, Branka T, Sekulic, Milka I, Milošević, Verica Lj., "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study" in Canadian Journal of Physiology and Pharmacology, 88, no. 4 (2010):497-500, https://doi.org/10.1139/Y10-020 . .