The antioxidant activity of the Calluna vulgaris (L.) Hull. extracts obtained by supercritical CO2 extraction
Аутори
Preradović, NevenaNakarada, Đura
Simonović Radosavljević, Jasna
Spasojević, Dragica
Radotić, Ksenija
Mojović, Miloš
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Calluna vulgaris (L.) Hull. is an evergreen perennial plant of the Ericaceae family possesses noteworthy pharmacological properties and has long been used in traditional medicine for
addressing many medical conditions [1]. A plethora of bioactive compounds have been
discovered in C. vulgaris, with phenolics being the primary source of its diverse health benefits,
including antioxidant, anti-inflammatory, and antimicrobial properties, among others [2, 3]. However, a detailed study of its antiradical activity has not been conducted before. Herein, the supercritical CO2 extraction of C. vulgaris branches has been employed to obtain an extract rich in polyphenolic compounds. Supercritical CO2 extraction, a green technology method, replaces classical solvent-based extraction, enabling the isolation of plant compounds in their purest form without degradation of the sample [4]. Owing to its high sensitivity and specificity,
Electron paramagnetic resonance (EPR) spectroscopy has been u...tilized to access the extract's scavenging activity against free radical species (DPPH and hydroxyl radicals). The spin-trapping technique was employed to detect activity against short-lived hydroxyl radicals.
The results demonstrate that the C. vulgaris branch extract is a significant free-radical scavenger. It exhibited remarkable selectivity in eliminating hydroxyl radicals (68.5% compared to 21.7% for DPPH), which is particularly noteworthy as hydroxyl radicals are considered the most reactive oxygen radical species, biologically relevant, and responsible for a number of pathologies. These findings underscore the therapeutic potential of C. vulgaris branch extract in combating oxidative stress-related medical conditions but also suggest possibilities for further investigation into its mechanism of action and formulation into pharmaceutical or nutraceutical products.
Кључне речи:
Calluna vulgaris / CO2 extraction / antioxidant activity / EPRИзвор:
3rd MOSBRI Scientific Conference, 2024, 140-Издавач:
- National Institute of Chemistry, Ljubljana
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200146 (Универзитет у Београду, Факултет за физичку хемију) (RS-MESTD-inst-2020-200146)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Preradović, Nevena AU - Nakarada, Đura AU - Simonović Radosavljević, Jasna AU - Spasojević, Dragica AU - Radotić, Ksenija AU - Mojović, Miloš PY - 2024 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/3231 AB - Calluna vulgaris (L.) Hull. is an evergreen perennial plant of the Ericaceae family possesses noteworthy pharmacological properties and has long been used in traditional medicine for addressing many medical conditions [1]. A plethora of bioactive compounds have been discovered in C. vulgaris, with phenolics being the primary source of its diverse health benefits, including antioxidant, anti-inflammatory, and antimicrobial properties, among others [2, 3]. However, a detailed study of its antiradical activity has not been conducted before. Herein, the supercritical CO2 extraction of C. vulgaris branches has been employed to obtain an extract rich in polyphenolic compounds. Supercritical CO2 extraction, a green technology method, replaces classical solvent-based extraction, enabling the isolation of plant compounds in their purest form without degradation of the sample [4]. Owing to its high sensitivity and specificity, Electron paramagnetic resonance (EPR) spectroscopy has been utilized to access the extract's scavenging activity against free radical species (DPPH and hydroxyl radicals). The spin-trapping technique was employed to detect activity against short-lived hydroxyl radicals. The results demonstrate that the C. vulgaris branch extract is a significant free-radical scavenger. It exhibited remarkable selectivity in eliminating hydroxyl radicals (68.5% compared to 21.7% for DPPH), which is particularly noteworthy as hydroxyl radicals are considered the most reactive oxygen radical species, biologically relevant, and responsible for a number of pathologies. These findings underscore the therapeutic potential of C. vulgaris branch extract in combating oxidative stress-related medical conditions but also suggest possibilities for further investigation into its mechanism of action and formulation into pharmaceutical or nutraceutical products. PB - National Institute of Chemistry, Ljubljana C3 - 3rd MOSBRI Scientific Conference T1 - The antioxidant activity of the Calluna vulgaris (L.) Hull. extracts obtained by supercritical CO2 extraction SP - 140 UR - https://hdl.handle.net/21.15107/rcub_rimsi_3231 ER -
@conference{ author = "Preradović, Nevena and Nakarada, Đura and Simonović Radosavljević, Jasna and Spasojević, Dragica and Radotić, Ksenija and Mojović, Miloš", year = "2024", abstract = "Calluna vulgaris (L.) Hull. is an evergreen perennial plant of the Ericaceae family possesses noteworthy pharmacological properties and has long been used in traditional medicine for addressing many medical conditions [1]. A plethora of bioactive compounds have been discovered in C. vulgaris, with phenolics being the primary source of its diverse health benefits, including antioxidant, anti-inflammatory, and antimicrobial properties, among others [2, 3]. However, a detailed study of its antiradical activity has not been conducted before. Herein, the supercritical CO2 extraction of C. vulgaris branches has been employed to obtain an extract rich in polyphenolic compounds. Supercritical CO2 extraction, a green technology method, replaces classical solvent-based extraction, enabling the isolation of plant compounds in their purest form without degradation of the sample [4]. Owing to its high sensitivity and specificity, Electron paramagnetic resonance (EPR) spectroscopy has been utilized to access the extract's scavenging activity against free radical species (DPPH and hydroxyl radicals). The spin-trapping technique was employed to detect activity against short-lived hydroxyl radicals. The results demonstrate that the C. vulgaris branch extract is a significant free-radical scavenger. It exhibited remarkable selectivity in eliminating hydroxyl radicals (68.5% compared to 21.7% for DPPH), which is particularly noteworthy as hydroxyl radicals are considered the most reactive oxygen radical species, biologically relevant, and responsible for a number of pathologies. These findings underscore the therapeutic potential of C. vulgaris branch extract in combating oxidative stress-related medical conditions but also suggest possibilities for further investigation into its mechanism of action and formulation into pharmaceutical or nutraceutical products.", publisher = "National Institute of Chemistry, Ljubljana", journal = "3rd MOSBRI Scientific Conference", title = "The antioxidant activity of the Calluna vulgaris (L.) Hull. extracts obtained by supercritical CO2 extraction", pages = "140", url = "https://hdl.handle.net/21.15107/rcub_rimsi_3231" }
Preradović, N., Nakarada, Đ., Simonović Radosavljević, J., Spasojević, D., Radotić, K.,& Mojović, M.. (2024). The antioxidant activity of the Calluna vulgaris (L.) Hull. extracts obtained by supercritical CO2 extraction. in 3rd MOSBRI Scientific Conference National Institute of Chemistry, Ljubljana., 140. https://hdl.handle.net/21.15107/rcub_rimsi_3231
Preradović N, Nakarada Đ, Simonović Radosavljević J, Spasojević D, Radotić K, Mojović M. The antioxidant activity of the Calluna vulgaris (L.) Hull. extracts obtained by supercritical CO2 extraction. in 3rd MOSBRI Scientific Conference. 2024;:140. https://hdl.handle.net/21.15107/rcub_rimsi_3231 .
Preradović, Nevena, Nakarada, Đura, Simonović Radosavljević, Jasna, Spasojević, Dragica, Radotić, Ksenija, Mojović, Miloš, "The antioxidant activity of the Calluna vulgaris (L.) Hull. extracts obtained by supercritical CO2 extraction" in 3rd MOSBRI Scientific Conference (2024):140, https://hdl.handle.net/21.15107/rcub_rimsi_3231 .