Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical
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2009
Authors
Spasojević, Ivan![](/themes/MirageRIMSI/images/orcid.png)
Mojović, Miloš
![](/themes/MirageRIMSI/images/orcid.png)
Blagojević, Duško
Spasic, Snežana D.
![](/themes/MirageRIMSI/images/orcid.png)
Jones, David R
Nikolić-Kokić, Aleksandra
![](/themes/MirageRIMSI/images/orcid.png)
Spasić, Mihajlo
![](/themes/MirageRIMSI/images/orcid.png)
Article (Published version)
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The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot sca...venging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.
Keywords:
Mannitol / Hydroxyl radical / Haber-Weiss reaction / Glucose / Fructose / EPR spectroscopySource:
Carbohydrate Research, 2009, 344, 1, 80-84Publisher:
- Elsevier Sci Ltd, Oxford
Funding / projects:
- The Role of Redox-Active Substances in the Maintenance of Homeostasis (RS-MESTD-MPN2006-2010-143034)
- Biofizička istraživanja membranskih procesa: interakcija membranskih receptora i kanala sa spoljašnjim faktorima i intracelularna regulacija (RS-MESTD-MPN2006-2010-143016)
DOI: 10.1016/j.carres.2008.09.025
ISSN: 0008-6215
PubMed: 18947823
WoS: 000262596200011
Scopus: 2-s2.0-57749111966
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Spasojević, Ivan AU - Mojović, Miloš AU - Blagojević, Duško AU - Spasic, Snežana D. AU - Jones, David R AU - Nikolić-Kokić, Aleksandra AU - Spasić, Mihajlo PY - 2009 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/321 AB - The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy. PB - Elsevier Sci Ltd, Oxford T2 - Carbohydrate Research T1 - Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical EP - 84 IS - 1 SP - 80 VL - 344 DO - 10.1016/j.carres.2008.09.025 ER -
@article{ author = "Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Spasic, Snežana D. and Jones, David R and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo", year = "2009", abstract = "The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Carbohydrate Research", title = "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical", pages = "84-80", number = "1", volume = "344", doi = "10.1016/j.carres.2008.09.025" }
Spasojević, I., Mojović, M., Blagojević, D., Spasic, S. D., Jones, D. R., Nikolić-Kokić, A.,& Spasić, M.. (2009). Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research Elsevier Sci Ltd, Oxford., 344(1), 80-84. https://doi.org/10.1016/j.carres.2008.09.025
Spasojević I, Mojović M, Blagojević D, Spasic SD, Jones DR, Nikolić-Kokić A, Spasić M. Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research. 2009;344(1):80-84. doi:10.1016/j.carres.2008.09.025 .
Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Spasic, Snežana D., Jones, David R, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical" in Carbohydrate Research, 344, no. 1 (2009):80-84, https://doi.org/10.1016/j.carres.2008.09.025 . .