High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds
Abstract
The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of poten...tially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated.
Keywords:
Seed germination / Manganese / Electron paramagnetic resonanceSource:
Environmental and Experimental Botany, 2008, 64, 3, 322-324Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- Svetlosna i hormonalna kontrola rastenja i razvića biljaka, razmnožavanje in vitro i ex situ zaštita retkih i ugroženih vrsta (RS-143031)
- Biofizička istraživanja membranskih procesa: interakcija membranskih receptora i kanala sa spoljašnjim faktorima i intracelularna regulacija (RS-143016)
- Regulacija antioksidativnog metabolizma biljaka u toku rastenja, infekcije patogenima i delovanja abiotičkog stresa: mehanizmi transporta, signalizacije i otpornosti (RS-143020)
DOI: 10.1016/j.envexpbot.2008.07.001
ISSN: 0098-8472
WoS: 000260659300015
Scopus: 2-s2.0-53149136548
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Todorović, Sladana AU - Giba, Zlatko AU - Bačić, Goran AU - Nikolic, Miroslav AU - Grubisic, Dragoljub PY - 2008 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/299 AB - The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Environmental and Experimental Botany T1 - High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds EP - 324 IS - 3 SP - 322 VL - 64 DO - 10.1016/j.envexpbot.2008.07.001 ER -
@article{ author = "Todorović, Sladana and Giba, Zlatko and Bačić, Goran and Nikolic, Miroslav and Grubisic, Dragoljub", year = "2008", abstract = "The effect of high Mn2+ content on Centaurium pulchellum seed germination has been investigated. Seeds containing extremely high Mn2+ content were produced by culturing single-node flowering explants for 2 months in the MS-media, supplemented with Mn in concentrations ranging from 1 to 10,000 mu M. Although the seeds displayed the capacity to accumulate high amount of Mn, their germination was undisturbed. EPR spectroscopy was used to measure the ratio of free (aqueous) Mn to bound Mn and it was found that over 97% of total Mn was in the bound form. With elevating the external Mn supply, seed Mn concentration also increased, but the proportion of free Mn2+ fraction decreased from 3% in the control (1 mu M Mn) to 0.35% and 0.15% in high Mn supply (1000 mu M and 10,000 mu M, respectively). These results suggest that an elevation of internal Mn concentration in seeds is associated with increased Mn binding pools, hence Mn remains bound during germination. Consequently, the action of potentially harmful Mn2+ ions, which may generate ROS and affect seed viability, is alleviated.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Environmental and Experimental Botany", title = "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds", pages = "324-322", number = "3", volume = "64", doi = "10.1016/j.envexpbot.2008.07.001" }
Todorović, S., Giba, Z., Bačić, G., Nikolic, M.,& Grubisic, D.. (2008). High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany Pergamon-Elsevier Science Ltd, Oxford., 64(3), 322-324. https://doi.org/10.1016/j.envexpbot.2008.07.001
Todorović S, Giba Z, Bačić G, Nikolic M, Grubisic D. High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds. in Environmental and Experimental Botany. 2008;64(3):322-324. doi:10.1016/j.envexpbot.2008.07.001 .
Todorović, Sladana, Giba, Zlatko, Bačić, Goran, Nikolic, Miroslav, Grubisic, Dragoljub, "High seed Mn content does not affect germination of in vitro produced Centaurium pulchellum seeds" in Environmental and Experimental Botany, 64, no. 3 (2008):322-324, https://doi.org/10.1016/j.envexpbot.2008.07.001 . .