Apoptosis of the oocytes of hypothyroid neonatal rats
Authors
Danilović Luković, JelenaMarković, Danica
Todorović, Vera
Drndarević, Neda
Roksandić, Dragutin
Radovanovic, Anita
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Background: The aim of our study was to examine the effect of mild maternal hypothyroidism on the apoptosis of the oocytes in the ovaries of rats in the early postnatal period during formation of oocytes and follicles.
Materials and Methods: Hypothyroidism was induced in pregnant and lactating rats by feeding 1.5mg/L propylthiouracil (PTU) through drinking water. Ovaries were obtained from newborns on postnatal day (PND) 0, 4 and 7. The degree of oocyte apoptosis was measured from the largest cross sections (n=2) from each ovary using by Colorimetric - TdT (terminal deoxynucleoid transferase) Enzyme in situ cell death detection kit (Merck).
Results: This preliminary result suggests that hypothyroidism induce increase of the total oocyte number from 4. day in the experimental group while the number of apoptotic oocytes is the lowest that day. However, the highest percentage of apoptosis is noticed on day 0. in control group.
Conclusion: In our further investigation we will notice whe...ther the morphological changes induced on the oocytes appear before or after development of primordial follicles as a result of the impacts of maternal hypothyroidism on folliculogenesis of their offspring.
Keywords:
maternal hypothyroidism / propylthiouracil (PTU) / oocytes / ovary / apoptosis / newbornSource:
5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist, 2013, 36-36Publisher:
- Medical University of Graz, Austria
Funding / projects:
- Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing (RS-MESTD-Basic Research (BR or ON)-175061)
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Institut za multidisciplinarna istraživanjaTY - CONF AU - Danilović Luković, Jelena AU - Marković, Danica AU - Todorović, Vera AU - Drndarević, Neda AU - Roksandić, Dragutin AU - Radovanovic, Anita PY - 2013 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2737 AB - Background: The aim of our study was to examine the effect of mild maternal hypothyroidism on the apoptosis of the oocytes in the ovaries of rats in the early postnatal period during formation of oocytes and follicles. Materials and Methods: Hypothyroidism was induced in pregnant and lactating rats by feeding 1.5mg/L propylthiouracil (PTU) through drinking water. Ovaries were obtained from newborns on postnatal day (PND) 0, 4 and 7. The degree of oocyte apoptosis was measured from the largest cross sections (n=2) from each ovary using by Colorimetric - TdT (terminal deoxynucleoid transferase) Enzyme in situ cell death detection kit (Merck). Results: This preliminary result suggests that hypothyroidism induce increase of the total oocyte number from 4. day in the experimental group while the number of apoptotic oocytes is the lowest that day. However, the highest percentage of apoptosis is noticed on day 0. in control group. Conclusion: In our further investigation we will notice whether the morphological changes induced on the oocytes appear before or after development of primordial follicles as a result of the impacts of maternal hypothyroidism on folliculogenesis of their offspring. PB - Medical University of Graz, Austria C3 - 5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist T1 - Apoptosis of the oocytes of hypothyroid neonatal rats EP - 36 SP - 36 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2737 ER -
@conference{ author = "Danilović Luković, Jelena and Marković, Danica and Todorović, Vera and Drndarević, Neda and Roksandić, Dragutin and Radovanovic, Anita", year = "2013", abstract = "Background: The aim of our study was to examine the effect of mild maternal hypothyroidism on the apoptosis of the oocytes in the ovaries of rats in the early postnatal period during formation of oocytes and follicles. Materials and Methods: Hypothyroidism was induced in pregnant and lactating rats by feeding 1.5mg/L propylthiouracil (PTU) through drinking water. Ovaries were obtained from newborns on postnatal day (PND) 0, 4 and 7. The degree of oocyte apoptosis was measured from the largest cross sections (n=2) from each ovary using by Colorimetric - TdT (terminal deoxynucleoid transferase) Enzyme in situ cell death detection kit (Merck). Results: This preliminary result suggests that hypothyroidism induce increase of the total oocyte number from 4. day in the experimental group while the number of apoptotic oocytes is the lowest that day. However, the highest percentage of apoptosis is noticed on day 0. in control group. Conclusion: In our further investigation we will notice whether the morphological changes induced on the oocytes appear before or after development of primordial follicles as a result of the impacts of maternal hypothyroidism on folliculogenesis of their offspring.", publisher = "Medical University of Graz, Austria", journal = "5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist", title = "Apoptosis of the oocytes of hypothyroid neonatal rats", pages = "36-36", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2737" }
Danilović Luković, J., Marković, D., Todorović, V., Drndarević, N., Roksandić, D.,& Radovanovic, A.. (2013). Apoptosis of the oocytes of hypothyroid neonatal rats. in 5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist Medical University of Graz, Austria., 36-36. https://hdl.handle.net/21.15107/rcub_rimsi_2737
Danilović Luković J, Marković D, Todorović V, Drndarević N, Roksandić D, Radovanovic A. Apoptosis of the oocytes of hypothyroid neonatal rats. in 5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist. 2013;:36-36. https://hdl.handle.net/21.15107/rcub_rimsi_2737 .
Danilović Luković, Jelena, Marković, Danica, Todorović, Vera, Drndarević, Neda, Roksandić, Dragutin, Radovanovic, Anita, "Apoptosis of the oocytes of hypothyroid neonatal rats" in 5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist (2013):36-36, https://hdl.handle.net/21.15107/rcub_rimsi_2737 .