Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules
Authors
Dinić, ZoranUgrinović, Milan
Bosnić, Predrag
Mijatović, Mirjana
Zdravković, Jasmina
Miladinović, Miroslav
Jošić, Dragana
Article (Published version)
,
Institut za ratarstvo i povrtarstvo, Novi Sad
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Show full item recordAbstract
Phosphorus (P) is one of the major essential macronutrients for plant growth. Phosphate
solubilizing bacteria (PCB) is a group of heterotrophic bacteria capable of solubilizing organic and
inorganic phosphorus from insoluble compounds, releasing phosphate available for plant uptake. In this study, endophytic bacteria were isolated from nodules of French bean grown in different intercropping systems. Only 14 out of 158 endophytic isolates were able to solubilize tricalcium phosphate (TCP). None of them were observed to solubilize FePO4 and AlPO4 Eight isolates formed solubilization zones larger than 3 mm and were used for further test. The TCP solubilization was measured in liquid Pikovskaya medium and ranged from 14-182 μgml -1 of soluble phosphorus. TCP solubilization was correlated with acidity and pH decreasing from 7.4 to 5.57 and 4.91 for the lowest and the highest solubilizer, respectively, were observed. The most efficient isolate MP18 was selected and identified as Pseudomona...s sp. according to 16S rRNA gene analysis.
Keywords:
Endophytic bacteria, French bean nodules, phosphate solubilizing bacteria, Pseudomonas, soil pHSource:
Ratarstvo i povrtarstvo, 2014, 51Publisher:
- Institut za ratarstvo i povrtarstvo, Novi Sad
Funding / projects:
- New indigenous bacterial isolates Lysobacter and Pseudomonas as an important source of metabolites useful for biotechnology, plant growth stimulation and disease control: from isolates to inoculants (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46007)
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Dinić, Zoran AU - Ugrinović, Milan AU - Bosnić, Predrag AU - Mijatović, Mirjana AU - Zdravković, Jasmina AU - Miladinović, Miroslav AU - Jošić, Dragana PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2711 AB - Phosphorus (P) is one of the major essential macronutrients for plant growth. Phosphate solubilizing bacteria (PCB) is a group of heterotrophic bacteria capable of solubilizing organic and inorganic phosphorus from insoluble compounds, releasing phosphate available for plant uptake. In this study, endophytic bacteria were isolated from nodules of French bean grown in different intercropping systems. Only 14 out of 158 endophytic isolates were able to solubilize tricalcium phosphate (TCP). None of them were observed to solubilize FePO4 and AlPO4 Eight isolates formed solubilization zones larger than 3 mm and were used for further test. The TCP solubilization was measured in liquid Pikovskaya medium and ranged from 14-182 μgml -1 of soluble phosphorus. TCP solubilization was correlated with acidity and pH decreasing from 7.4 to 5.57 and 4.91 for the lowest and the highest solubilizer, respectively, were observed. The most efficient isolate MP18 was selected and identified as Pseudomonas sp. according to 16S rRNA gene analysis. PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Ratarstvo i povrtarstvo T1 - Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules IS - 51 DO - 10.5937/ratpov51-6222 ER -
@article{ author = "Dinić, Zoran and Ugrinović, Milan and Bosnić, Predrag and Mijatović, Mirjana and Zdravković, Jasmina and Miladinović, Miroslav and Jošić, Dragana", year = "2014", abstract = "Phosphorus (P) is one of the major essential macronutrients for plant growth. Phosphate solubilizing bacteria (PCB) is a group of heterotrophic bacteria capable of solubilizing organic and inorganic phosphorus from insoluble compounds, releasing phosphate available for plant uptake. In this study, endophytic bacteria were isolated from nodules of French bean grown in different intercropping systems. Only 14 out of 158 endophytic isolates were able to solubilize tricalcium phosphate (TCP). None of them were observed to solubilize FePO4 and AlPO4 Eight isolates formed solubilization zones larger than 3 mm and were used for further test. The TCP solubilization was measured in liquid Pikovskaya medium and ranged from 14-182 μgml -1 of soluble phosphorus. TCP solubilization was correlated with acidity and pH decreasing from 7.4 to 5.57 and 4.91 for the lowest and the highest solubilizer, respectively, were observed. The most efficient isolate MP18 was selected and identified as Pseudomonas sp. according to 16S rRNA gene analysis.", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Ratarstvo i povrtarstvo", title = "Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules", number = "51", doi = "10.5937/ratpov51-6222" }
Dinić, Z., Ugrinović, M., Bosnić, P., Mijatović, M., Zdravković, J., Miladinović, M.,& Jošić, D.. (2014). Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules. in Ratarstvo i povrtarstvo Institut za ratarstvo i povrtarstvo, Novi Sad.(51). https://doi.org/10.5937/ratpov51-6222
Dinić Z, Ugrinović M, Bosnić P, Mijatović M, Zdravković J, Miladinović M, Jošić D. Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules. in Ratarstvo i povrtarstvo. 2014;(51). doi:10.5937/ratpov51-6222 .
Dinić, Zoran, Ugrinović, Milan, Bosnić, Predrag, Mijatović, Mirjana, Zdravković, Jasmina, Miladinović, Miroslav, Jošić, Dragana, "Solubilization of inorganic phosphate by endophytic Pseudomonas sp. from French bean nodules" in Ratarstvo i povrtarstvo, no. 51 (2014), https://doi.org/10.5937/ratpov51-6222 . .