Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth
Samo za registrovane korisnike
2008
Autori
Dragišić Maksimović, JelenaMaksimović, Vuk
Živanović, Branka D.
Hadzi-Tasković Sukalović, Vesna
Vuletic, Mirjana
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increase...d lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid.
Ključne reči:
Phenolic compounds / Peroxidases / Isoelectric focusing / Growing zones / Apoplastic fluidIzvor:
Plant Science, 2008, 175, 5, 656-662Izdavač:
- Elsevier Ireland Ltd, Clare
Finansiranje / projekti:
- Biofizička istraživanja membranskih procesa: interakcija membranskih receptora i kanala sa spoljašnjim faktorima i intracelularna regulacija (RS-MESTD-MPN2006-2010-143016)
- Regulacija antioksidativnog metabolizma biljaka u toku rastenja, infekcije patogenima i delovanja abiotičkog stresa: mehanizmi transporta, signalizacije i otpornosti (RS-MESTD-MPN2006-2010-143020)
DOI: 10.1016/j.plantsci.2008.06.015
ISSN: 0168-9452
WoS: 000260160600007
Scopus: 2-s2.0-51549106657
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Dragišić Maksimović, Jelena AU - Maksimović, Vuk AU - Živanović, Branka D. AU - Hadzi-Tasković Sukalović, Vesna AU - Vuletic, Mirjana PY - 2008 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/266 AB - Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increased lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid. PB - Elsevier Ireland Ltd, Clare T2 - Plant Science T1 - Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth EP - 662 IS - 5 SP - 656 VL - 175 DO - 10.1016/j.plantsci.2008.06.015 ER -
@article{ author = "Dragišić Maksimović, Jelena and Maksimović, Vuk and Živanović, Branka D. and Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana", year = "2008", abstract = "Apoplastic fluid originated from maize (Zea mays L) leaf and root growing and non-growing regions were analyzed for peroxidase (POD) activities and phenols content. The samples were obtained by two different methods, as apoplastic washing fluid (AWF) from excised leaf and root segments, and as root apoplastic fluid (RAF) collected from the surface of intact roots by filter papers. AWF and RAF were tested for peroxidative and oxidative activity of POD, being lowest in the growing zones and gradually increasing toward root base and leaf tip. Isoelectric focusing of AWF and RAF revealed the differences pronounced in cationic group of peroxidases. Quantitative and qualitative analyzes of phenolic compounds from root and leaf apoplastic fluid was performed by HPLC. The increase of peroxidase activities was parallel to the increase of coniferyl alcohol, as well as other phenolic compound concentrations with root and leaf age, indicating that increased phenolic concentrations reflect increased lignin production during maturation. The data presented in this paper indicate that the method for getting RAF using filter papers is a more reliable procedure than infiltration/centrifugation method for studying processes which take place in root and leaf apoplastic fluid.", publisher = "Elsevier Ireland Ltd, Clare", journal = "Plant Science", title = "Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth", pages = "662-656", number = "5", volume = "175", doi = "10.1016/j.plantsci.2008.06.015" }
Dragišić Maksimović, J., Maksimović, V., Živanović, Branka D., Hadzi-Tasković Sukalović, V.,& Vuletic, M.. (2008). Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth. in Plant Science Elsevier Ireland Ltd, Clare., 175(5), 656-662. https://doi.org/10.1016/j.plantsci.2008.06.015
Dragišić Maksimović J, Maksimović V, Živanović, Branka D., Hadzi-Tasković Sukalović V, Vuletic M. Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth. in Plant Science. 2008;175(5):656-662. doi:10.1016/j.plantsci.2008.06.015 .
Dragišić Maksimović, Jelena, Maksimović, Vuk, Živanović, Branka D., Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana, "Peroxidase activity and phenolic compounds content in maize root and leaf apoplast, and their association with growth" in Plant Science, 175, no. 5 (2008):656-662, https://doi.org/10.1016/j.plantsci.2008.06.015 . .