Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species
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Sunjog, Karolina![](/themes/MirageRIMSI/images/orcid.png)
Kostić, Jovana
![](/themes/MirageRIMSI/images/orcid.png)
Lakota, Ajla
Aksović, Nevena
Kolarević, Stoimir
Gacic, Zoran
Lenhardt, Mirjana
![](/themes/MirageRIMSI/images/orcid.png)
Vuković-Gačić, Branka
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This work represents the study of quality of the Danube River, in Belgrade, at the Višnjica
site, by analyzing the genotoxicity of water by using fish as bioindicators. With a population
of about 2 million inhabitants (Belgrade) and poor legislation regarding the discharge of
wastewaters, monitoring is the first step in approaching this serious problem. To obtain
information on the genotoxicity of water as a measure of DNA damage, the comet assay,
which detects the effects of exposure, and micronucleus test, which detects permanent
effect, were used. For the comet assay we have used three types of cells: blood, liver, and
gills, while the micronucleus test was done on blood cells. The fish used as bioindicators are
the common perch (Perca fluviatilis), vimba bream (Vimba vimba), common barbell (Barbus
barbus), and white bream (Blicca bjoerkna).
Regarding the comet assay, significant difference in DNA damage was observed
between species in all tested tissues. Vimba bream and ...common barbell had the
highest level of DNA damage in gills, white bream in liver and common perch in blood.
In the case of the micronucleus test, the highest number of micronuclei was detected in
white bream blood cells. The obtained results showed that there is no correlation between
comet assay and the micronucleus test suggesting the importance of performing different
bioassays on multiple types of fish tissues in order to find the best biomarker for assessing
the genotoxic potential of water.
Keywords:
ecogenotoxicology / DNA damage / comet assay / micronucleus testSource:
6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia, 2019, 127-Publisher:
- Serbian Genetic Society, Belgrade, Serbia
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Institut za multidisciplinarna istraživanjaTY - CONF AU - Sunjog, Karolina AU - Kostić, Jovana AU - Lakota, Ajla AU - Aksović, Nevena AU - Kolarević, Stoimir AU - Gacic, Zoran AU - Lenhardt, Mirjana AU - Vuković-Gačić, Branka PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2462 AB - This work represents the study of quality of the Danube River, in Belgrade, at the Višnjica site, by analyzing the genotoxicity of water by using fish as bioindicators. With a population of about 2 million inhabitants (Belgrade) and poor legislation regarding the discharge of wastewaters, monitoring is the first step in approaching this serious problem. To obtain information on the genotoxicity of water as a measure of DNA damage, the comet assay, which detects the effects of exposure, and micronucleus test, which detects permanent effect, were used. For the comet assay we have used three types of cells: blood, liver, and gills, while the micronucleus test was done on blood cells. The fish used as bioindicators are the common perch (Perca fluviatilis), vimba bream (Vimba vimba), common barbell (Barbus barbus), and white bream (Blicca bjoerkna). Regarding the comet assay, significant difference in DNA damage was observed between species in all tested tissues. Vimba bream and common barbell had the highest level of DNA damage in gills, white bream in liver and common perch in blood. In the case of the micronucleus test, the highest number of micronuclei was detected in white bream blood cells. The obtained results showed that there is no correlation between comet assay and the micronucleus test suggesting the importance of performing different bioassays on multiple types of fish tissues in order to find the best biomarker for assessing the genotoxic potential of water. PB - Serbian Genetic Society, Belgrade, Serbia C3 - 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia T1 - Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species SP - 127 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2462 ER -
@conference{ author = "Sunjog, Karolina and Kostić, Jovana and Lakota, Ajla and Aksović, Nevena and Kolarević, Stoimir and Gacic, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka", year = "2019", abstract = "This work represents the study of quality of the Danube River, in Belgrade, at the Višnjica site, by analyzing the genotoxicity of water by using fish as bioindicators. With a population of about 2 million inhabitants (Belgrade) and poor legislation regarding the discharge of wastewaters, monitoring is the first step in approaching this serious problem. To obtain information on the genotoxicity of water as a measure of DNA damage, the comet assay, which detects the effects of exposure, and micronucleus test, which detects permanent effect, were used. For the comet assay we have used three types of cells: blood, liver, and gills, while the micronucleus test was done on blood cells. The fish used as bioindicators are the common perch (Perca fluviatilis), vimba bream (Vimba vimba), common barbell (Barbus barbus), and white bream (Blicca bjoerkna). Regarding the comet assay, significant difference in DNA damage was observed between species in all tested tissues. Vimba bream and common barbell had the highest level of DNA damage in gills, white bream in liver and common perch in blood. In the case of the micronucleus test, the highest number of micronuclei was detected in white bream blood cells. The obtained results showed that there is no correlation between comet assay and the micronucleus test suggesting the importance of performing different bioassays on multiple types of fish tissues in order to find the best biomarker for assessing the genotoxic potential of water.", publisher = "Serbian Genetic Society, Belgrade, Serbia", journal = "6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia", title = "Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species", pages = "127", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2462" }
Sunjog, K., Kostić, J., Lakota, A., Aksović, N., Kolarević, S., Gacic, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species. in 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia Serbian Genetic Society, Belgrade, Serbia., 127. https://hdl.handle.net/21.15107/rcub_rimsi_2462
Sunjog K, Kostić J, Lakota A, Aksović N, Kolarević S, Gacic Z, Lenhardt M, Vuković-Gačić B. Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species. in 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia. 2019;:127. https://hdl.handle.net/21.15107/rcub_rimsi_2462 .
Sunjog, Karolina, Kostić, Jovana, Lakota, Ajla, Aksović, Nevena, Kolarević, Stoimir, Gacic, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species" in 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia (2019):127, https://hdl.handle.net/21.15107/rcub_rimsi_2462 .