Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia
Authors
Kostić-Vuković, JovanaKolarević, Stoimir
Sunjog, Karolina
Subotić, Srđan
Višnjić Jeftić, Željka
Raskovic, Bozidar
Poleksic, Vesna
Vuković-Gačić, Branka
Lenhardt, Mirjana
Article (Published version)
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In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a sign...ificantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.
Keywords:
Metals accumulation / Erythrocyte morphometry / Comet assay / Micronucleus / Histopathology / HepG2Source:
Springer Nature, Ecotoxicology 32, 583–597 (2023), 2023, 32, 583-597Publisher:
- Springer Nature
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Kostić-Vuković, Jovana AU - Kolarević, Stoimir AU - Sunjog, Karolina AU - Subotić, Srđan AU - Višnjić Jeftić, Željka AU - Raskovic, Bozidar AU - Poleksic, Vesna AU - Vuković-Gačić, Branka AU - Lenhardt, Mirjana PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2216 AB - In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems. PB - Springer Nature T2 - Springer Nature, Ecotoxicology 32, 583–597 (2023) T1 - Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia EP - 597 IS - 32 SP - 583 DO - https://doi.org/10.1007/s10646-023-02663-6 ER -
@article{ author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Subotić, Srđan and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Vuković-Gačić, Branka and Lenhardt, Mirjana", year = "2023", abstract = "In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.", publisher = "Springer Nature", journal = "Springer Nature, Ecotoxicology 32, 583–597 (2023)", title = "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia", pages = "597-583", number = "32", doi = "https://doi.org/10.1007/s10646-023-02663-6" }
Kostić-Vuković, J., Kolarević, S., Sunjog, K., Subotić, S., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Vuković-Gačić, B.,& Lenhardt, M.. (2023). Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Springer Nature, Ecotoxicology 32, 583–597 (2023) Springer Nature.(32), 583-597. https://doi.org/https://doi.org/10.1007/s10646-023-02663-6
Kostić-Vuković J, Kolarević S, Sunjog K, Subotić S, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Vuković-Gačić B, Lenhardt M. Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Springer Nature, Ecotoxicology 32, 583–597 (2023). 2023;(32):583-597. doi:https://doi.org/10.1007/s10646-023-02663-6 .
Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Subotić, Srđan, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia" in Springer Nature, Ecotoxicology 32, 583–597 (2023), no. 32 (2023):583-597, https://doi.org/https://doi.org/10.1007/s10646-023-02663-6 . .