Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti
Authors
Buntić, AnetaStajkovic-Srbinovic, Olivera
Milić, Marija
Dubljanin, Tijana
Đorđe, Kuzmanović
Knezevic, Magdalena
Delic, Dusica
Conference object (Published version)
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Miscanthus × giganteus, as a lignocellulosic material, consists of about 30% xylan and represents a good basis for research as a possible raw material in the production of xylanases. Among the soil bacteria, rhizobium is rarely investigated as an enzyme producer. For the first time, Sinorhizobium meliloti strain 207 was used to obtain xylanase during submerged and solid fermentation using miscanthus biomass as a substrate. During submerged fermentation (28°C), the maximum xylanase activity was achieved after 48 h with 10% inoculum and H2SO4 substrate modification. The maximum xylanase activity of 1.215 U/mL was obtained during solid-state fermentation (28°C) by using also H2SO4 modified miscanthus biomass which was moistened with distillate water. The crude enzyme, produced by strain 207, could be further used in eco-friendly processes of lignocellulose material bioconversion to useful products.
Keywords:
agroindustrial waste / xylanase activity / solid-state fermentation / Sinorhizobium melilotiSource:
1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection, 2019, 151-163Publisher:
- The Balkans Scientific Center of the Russian Academy of Natural Sciences
Funding / projects:
- Application of biotechnological methods for sustainable exploitation of by-products of agro-industry (RS-MESTD-Technological Development (TD or TR)-31035)
- Study of the effects of soil and irrigation water quality on more efficient agricultural crop production and environment protection (RS-MESTD-Technological Development (TD or TR)-37006)
Note:
- Саопштење са међународног скупа штампано у целини (M33)
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - CONF AU - Buntić, Aneta AU - Stajkovic-Srbinovic, Olivera AU - Milić, Marija AU - Dubljanin, Tijana AU - Đorđe, Kuzmanović AU - Knezevic, Magdalena AU - Delic, Dusica PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2156 AB - Miscanthus × giganteus, as a lignocellulosic material, consists of about 30% xylan and represents a good basis for research as a possible raw material in the production of xylanases. Among the soil bacteria, rhizobium is rarely investigated as an enzyme producer. For the first time, Sinorhizobium meliloti strain 207 was used to obtain xylanase during submerged and solid fermentation using miscanthus biomass as a substrate. During submerged fermentation (28°C), the maximum xylanase activity was achieved after 48 h with 10% inoculum and H2SO4 substrate modification. The maximum xylanase activity of 1.215 U/mL was obtained during solid-state fermentation (28°C) by using also H2SO4 modified miscanthus biomass which was moistened with distillate water. The crude enzyme, produced by strain 207, could be further used in eco-friendly processes of lignocellulose material bioconversion to useful products. PB - The Balkans Scientific Center of the Russian Academy of Natural Sciences C3 - 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection T1 - Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti VL - 151-163 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2156 ER -
@conference{ author = "Buntić, Aneta and Stajkovic-Srbinovic, Olivera and Milić, Marija and Dubljanin, Tijana and Đorđe, Kuzmanović and Knezevic, Magdalena and Delic, Dusica", year = "2019", abstract = "Miscanthus × giganteus, as a lignocellulosic material, consists of about 30% xylan and represents a good basis for research as a possible raw material in the production of xylanases. Among the soil bacteria, rhizobium is rarely investigated as an enzyme producer. For the first time, Sinorhizobium meliloti strain 207 was used to obtain xylanase during submerged and solid fermentation using miscanthus biomass as a substrate. During submerged fermentation (28°C), the maximum xylanase activity was achieved after 48 h with 10% inoculum and H2SO4 substrate modification. The maximum xylanase activity of 1.215 U/mL was obtained during solid-state fermentation (28°C) by using also H2SO4 modified miscanthus biomass which was moistened with distillate water. The crude enzyme, produced by strain 207, could be further used in eco-friendly processes of lignocellulose material bioconversion to useful products.", publisher = "The Balkans Scientific Center of the Russian Academy of Natural Sciences", journal = "1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection", title = "Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti", volume = "151-163", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2156" }
Buntić, A., Stajkovic-Srbinovic, O., Milić, M., Dubljanin, T., Đorđe, K., Knezevic, M.,& Delic, D.. (2019). Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti. in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection The Balkans Scientific Center of the Russian Academy of Natural Sciences., 151-163. https://hdl.handle.net/21.15107/rcub_rimsi_2156
Buntić A, Stajkovic-Srbinovic O, Milić M, Dubljanin T, Đorđe K, Knezevic M, Delic D. Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti. in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection. 2019;151-163. https://hdl.handle.net/21.15107/rcub_rimsi_2156 .
Buntić, Aneta, Stajkovic-Srbinovic, Olivera, Milić, Marija, Dubljanin, Tijana, Đorđe, Kuzmanović, Knezevic, Magdalena, Delic, Dusica, "Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti" in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection, 151-163 (2019), https://hdl.handle.net/21.15107/rcub_rimsi_2156 .