Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy
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2023
Authors
Gianoncelli, AlessandraŽižić, Milan
Bonanni, Valentina
Birarda, Giovanni
Bedolla Orozco, Diana Eva
Vogel-Mikus, Katarina
Article (Updated Version)
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.The radiation damage issue consequent to soft X-rays’ exposure is still an important aspect to be contemplated in soft X-ray microscopy. The work presented here is part of a more
extended investigation on the topic and targets the effects of soft X-rays exposure on plant tissues.
The aftermaths of soft X-rays’ exposure were evaluated by FTIR spectromicroscopy by comparing irradiated and non-irradiated areas on similar plant structural features, one day and ten days after irradiation, to highlight also a possible time-dependent behavior consequent to air exposure.
Our results show partial degradation of lignocellulosic complex and oxidation of cellulose and most probably of hemicellulose; lignin moiety of cell walls in vascular tissue however remains stable.
Comparing the current study with our previous works it appears that, as expected, plant tissues seem less prone to soft X-rays radiation damage compared to mammalian cells or tissues.
Keywords:
Radiation damage / soft X-rays / FTIR / plant rootsSource:
Journal of Electron Spectroscopy and Related Phenomena, 2023, 267, 147384-147391Publisher:
- Elsevier, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB UK
Funding / projects:
- ARIS P1-0212
- CERIC-ERIC Consortium_project_20222193
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Gianoncelli, Alessandra AU - Žižić, Milan AU - Bonanni, Valentina AU - Birarda, Giovanni AU - Bedolla Orozco, Diana Eva AU - Vogel-Mikus, Katarina PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2109 AB - .The radiation damage issue consequent to soft X-rays’ exposure is still an important aspect to be contemplated in soft X-ray microscopy. The work presented here is part of a more extended investigation on the topic and targets the effects of soft X-rays exposure on plant tissues. The aftermaths of soft X-rays’ exposure were evaluated by FTIR spectromicroscopy by comparing irradiated and non-irradiated areas on similar plant structural features, one day and ten days after irradiation, to highlight also a possible time-dependent behavior consequent to air exposure. Our results show partial degradation of lignocellulosic complex and oxidation of cellulose and most probably of hemicellulose; lignin moiety of cell walls in vascular tissue however remains stable. Comparing the current study with our previous works it appears that, as expected, plant tissues seem less prone to soft X-rays radiation damage compared to mammalian cells or tissues. PB - Elsevier, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB UK T2 - Journal of Electron Spectroscopy and Related Phenomena T1 - Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy EP - 147391 SP - 147384 VL - 267 DO - 10.1016/j.elspec.2023.147384 ER -
@article{ author = "Gianoncelli, Alessandra and Žižić, Milan and Bonanni, Valentina and Birarda, Giovanni and Bedolla Orozco, Diana Eva and Vogel-Mikus, Katarina", year = "2023", abstract = ".The radiation damage issue consequent to soft X-rays’ exposure is still an important aspect to be contemplated in soft X-ray microscopy. The work presented here is part of a more extended investigation on the topic and targets the effects of soft X-rays exposure on plant tissues. The aftermaths of soft X-rays’ exposure were evaluated by FTIR spectromicroscopy by comparing irradiated and non-irradiated areas on similar plant structural features, one day and ten days after irradiation, to highlight also a possible time-dependent behavior consequent to air exposure. Our results show partial degradation of lignocellulosic complex and oxidation of cellulose and most probably of hemicellulose; lignin moiety of cell walls in vascular tissue however remains stable. Comparing the current study with our previous works it appears that, as expected, plant tissues seem less prone to soft X-rays radiation damage compared to mammalian cells or tissues.", publisher = "Elsevier, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB UK", journal = "Journal of Electron Spectroscopy and Related Phenomena", title = "Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy", pages = "147391-147384", volume = "267", doi = "10.1016/j.elspec.2023.147384" }
Gianoncelli, A., Žižić, M., Bonanni, V., Birarda, G., Bedolla Orozco, D. E.,& Vogel-Mikus, K.. (2023). Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy. in Journal of Electron Spectroscopy and Related Phenomena Elsevier, The Boulevard, Langford Lane, Kidlington, Oxford OX5 1GB UK., 267, 147384-147391. https://doi.org/10.1016/j.elspec.2023.147384
Gianoncelli A, Žižić M, Bonanni V, Birarda G, Bedolla Orozco DE, Vogel-Mikus K. Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy. in Journal of Electron Spectroscopy and Related Phenomena. 2023;267:147384-147391. doi:10.1016/j.elspec.2023.147384 .
Gianoncelli, Alessandra, Žižić, Milan, Bonanni, Valentina, Birarda, Giovanni, Bedolla Orozco, Diana Eva, Vogel-Mikus, Katarina, "Soft X -rays radiation damage on plunge -frozen and freeze -dried maize root s evaluated by FTIR spectromicroscopy" in Journal of Electron Spectroscopy and Related Phenomena, 267 (2023):147384-147391, https://doi.org/10.1016/j.elspec.2023.147384 . .