GnRHR signaling in neuronal cells: in vitro and in vivo data
Samo za registrovane korisnike
2023
Autori
Milosevic, AnaMilosevic, Katarina
Nikolic, Ljiljana
Bogdanović Pristov, Jelena
Božić, Iva
Zivkovic, Anica
Lavrnja, Irena
Savic, Danijela
Janjic, Marija
Bjelobaba, Ivana
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide that controls
mammalian reproduction by acting on its receptor (GnRHR) expressed on pituitary
gonadotrope cells. While GnRHR signaling in gonadotropes is well described,
knowledge of GnRHR activation-related events at extrapituitary sites including
neurons is limited. It was proposed that GnRH analogs (GnRHa) induce distinct
changes in hippocampal gene expression, emotional processes, and cognitive
functions.
To explore neuronal GnRHR signaling we used the human neuroblastoma cell line
SH-SY5Y. Further, we explored the regional expression of Gnrhr in rat brain and
investigated the expression of several relevant genes in the hippocampus and preoptic
area of peripubertal male rats treated with GnRHa.
GNRHR is expressed in SH-SY5Y cell line, but its expression does not change after
adding GnRHa in the incubation media. Electrophysiological recordings confirmed
that GnRHa induced membrane depola...rization but could not evoke action potentials.
In the rat brain, Gnrhr expression could be detected in the hippocampus, amygdala,
and hypothalamus, including the preoptic area. Prolonged treatment of peripubertal
rats with GnRHa had no effect on the expression of genes in the hippocampus
previously shown to be affected in the sheep model of delayed puberty.
These results imply that neuronal GnRHR is either differently coupled (not coupled
with Gq/11 protein), or that its membrane density is too low to induce transcriptional
events. More investigation is needed to elucidate the role(s) of GnRH-GnRHR
signaling in the brain.
Ključne reči:
Gonadotropin-releasing hormone (GnRH) / ratIzvor:
8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS, 2023, 53-Izdavač:
- Belgrade: Serbian Neuroscience Society
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200007 (Univerzitet u Beogradu, Institut za biološka istraživanja 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200053 (Univerzitet u Beogradu, Institut za multidisciplinarna istraživanja) (RS-MESTD-inst-2020-200053)
Napomena:
- kategorija M64
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - CONF AU - Milosevic, Ana AU - Milosevic, Katarina AU - Nikolic, Ljiljana AU - Bogdanović Pristov, Jelena AU - Božić, Iva AU - Zivkovic, Anica AU - Lavrnja, Irena AU - Savic, Danijela AU - Janjic, Marija AU - Bjelobaba, Ivana PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2019 AB - Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide that controls mammalian reproduction by acting on its receptor (GnRHR) expressed on pituitary gonadotrope cells. While GnRHR signaling in gonadotropes is well described, knowledge of GnRHR activation-related events at extrapituitary sites including neurons is limited. It was proposed that GnRH analogs (GnRHa) induce distinct changes in hippocampal gene expression, emotional processes, and cognitive functions. To explore neuronal GnRHR signaling we used the human neuroblastoma cell line SH-SY5Y. Further, we explored the regional expression of Gnrhr in rat brain and investigated the expression of several relevant genes in the hippocampus and preoptic area of peripubertal male rats treated with GnRHa. GNRHR is expressed in SH-SY5Y cell line, but its expression does not change after adding GnRHa in the incubation media. Electrophysiological recordings confirmed that GnRHa induced membrane depolarization but could not evoke action potentials. In the rat brain, Gnrhr expression could be detected in the hippocampus, amygdala, and hypothalamus, including the preoptic area. Prolonged treatment of peripubertal rats with GnRHa had no effect on the expression of genes in the hippocampus previously shown to be affected in the sheep model of delayed puberty. These results imply that neuronal GnRHR is either differently coupled (not coupled with Gq/11 protein), or that its membrane density is too low to induce transcriptional events. More investigation is needed to elucidate the role(s) of GnRH-GnRHR signaling in the brain. PB - Belgrade: Serbian Neuroscience Society C3 - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS T1 - GnRHR signaling in neuronal cells: in vitro and in vivo data SP - 53 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2019 ER -
@conference{ author = "Milosevic, Ana and Milosevic, Katarina and Nikolic, Ljiljana and Bogdanović Pristov, Jelena and Božić, Iva and Zivkovic, Anica and Lavrnja, Irena and Savic, Danijela and Janjic, Marija and Bjelobaba, Ivana", year = "2023", abstract = "Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide that controls mammalian reproduction by acting on its receptor (GnRHR) expressed on pituitary gonadotrope cells. While GnRHR signaling in gonadotropes is well described, knowledge of GnRHR activation-related events at extrapituitary sites including neurons is limited. It was proposed that GnRH analogs (GnRHa) induce distinct changes in hippocampal gene expression, emotional processes, and cognitive functions. To explore neuronal GnRHR signaling we used the human neuroblastoma cell line SH-SY5Y. Further, we explored the regional expression of Gnrhr in rat brain and investigated the expression of several relevant genes in the hippocampus and preoptic area of peripubertal male rats treated with GnRHa. GNRHR is expressed in SH-SY5Y cell line, but its expression does not change after adding GnRHa in the incubation media. Electrophysiological recordings confirmed that GnRHa induced membrane depolarization but could not evoke action potentials. In the rat brain, Gnrhr expression could be detected in the hippocampus, amygdala, and hypothalamus, including the preoptic area. Prolonged treatment of peripubertal rats with GnRHa had no effect on the expression of genes in the hippocampus previously shown to be affected in the sheep model of delayed puberty. These results imply that neuronal GnRHR is either differently coupled (not coupled with Gq/11 protein), or that its membrane density is too low to induce transcriptional events. More investigation is needed to elucidate the role(s) of GnRH-GnRHR signaling in the brain.", publisher = "Belgrade: Serbian Neuroscience Society", journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS", title = "GnRHR signaling in neuronal cells: in vitro and in vivo data", pages = "53", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2019" }
Milosevic, A., Milosevic, K., Nikolic, L., Bogdanović Pristov, J., Božić, I., Zivkovic, A., Lavrnja, I., Savic, D., Janjic, M.,& Bjelobaba, I.. (2023). GnRHR signaling in neuronal cells: in vitro and in vivo data. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS Belgrade: Serbian Neuroscience Society., 53. https://hdl.handle.net/21.15107/rcub_rimsi_2019
Milosevic A, Milosevic K, Nikolic L, Bogdanović Pristov J, Božić I, Zivkovic A, Lavrnja I, Savic D, Janjic M, Bjelobaba I. GnRHR signaling in neuronal cells: in vitro and in vivo data. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:53. https://hdl.handle.net/21.15107/rcub_rimsi_2019 .
Milosevic, Ana, Milosevic, Katarina, Nikolic, Ljiljana, Bogdanović Pristov, Jelena, Božić, Iva, Zivkovic, Anica, Lavrnja, Irena, Savic, Danijela, Janjic, Marija, Bjelobaba, Ivana, "GnRHR signaling in neuronal cells: in vitro and in vivo data" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):53, https://hdl.handle.net/21.15107/rcub_rimsi_2019 .