Differential Polarization Laser Scanning Microscopy (DP-LSM) -Technique for Rapid Screening of Cell Walls of Different Plant Species

Abstract

DP-LS microscopy is a useful and easily applicable technique for rapid screening of cell wall structural order, by mapping the linear dichroism of cellulose fibrils. It allows screening and quantification of the alignment of cellulose fibrils, relative to one another, in cell walls. The goal was to provide new information about structural characteristics of the isolated cell walls among species which may be a basis for their possible applications, such as biofuel and biomaterial industry, but also to understand what plant response to stress is. The DP-LSM method is shown to be a simple and rapid way to check the structural complexity in the cell wall through cellulose labeling.