XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending
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2022
Authors
Prokopijević, MilošSimonović Radosavljević, Jasna
Spasojević, Dragica
Vojisavljević, Katarina
Radotić, Ksenija
Mitrović, Aleksandra Lj.
Article (Published version)
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Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood (TW). Typical TW fibers, gelatinous fibers (G-fibers), are characterized by an inner gelatinous cell wall layer (G-layer). Xyloglucan endotransglycosylases (XETs) was proposed as the essential enzyme in cell wall modifications in TW, by making xyloglucan (XG) cross-links between G- and S2-layers in G-fibers, and thus maintaining their contact. The etermination of TW presence in a sample is of great importance for the forest products industry, biofuel production, and tree physiology studies. However, TW is not easy to detect visually. The colorimetric assay for XET activity determination as an indicator of TW presence in a sample was tested on powdered stem segments of juvenile Populus x euramericana trees exposed to severe long-term static bending. In parallel, histochemical and ultrastructural characterization of stem samples of bent and control trees was performed. The tested colorimetric assay fo...r XET activity determination could be suggested as a useful and easily applicable tool for fast screening of powdered wood samples for the presence of TW.
Keywords:
Populus x euramericana cl. NS 11-8 / tension wood / xyloglucan endotransglycosylasesSource:
Holzforschung, 2022, 76, 6, 2500-2505Publisher:
- De Gruyter
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Prokopijević, Miloš AU - Simonović Radosavljević, Jasna AU - Spasojević, Dragica AU - Vojisavljević, Katarina AU - Radotić, Ksenija AU - Mitrović, Aleksandra Lj. PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1773 AB - Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood (TW). Typical TW fibers, gelatinous fibers (G-fibers), are characterized by an inner gelatinous cell wall layer (G-layer). Xyloglucan endotransglycosylases (XETs) was proposed as the essential enzyme in cell wall modifications in TW, by making xyloglucan (XG) cross-links between G- and S2-layers in G-fibers, and thus maintaining their contact. The etermination of TW presence in a sample is of great importance for the forest products industry, biofuel production, and tree physiology studies. However, TW is not easy to detect visually. The colorimetric assay for XET activity determination as an indicator of TW presence in a sample was tested on powdered stem segments of juvenile Populus x euramericana trees exposed to severe long-term static bending. In parallel, histochemical and ultrastructural characterization of stem samples of bent and control trees was performed. The tested colorimetric assay for XET activity determination could be suggested as a useful and easily applicable tool for fast screening of powdered wood samples for the presence of TW. PB - De Gruyter T2 - Holzforschung T1 - XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending EP - 2505 IS - 6 SP - 2500 VL - 76 DO - 10.1515/hf-2021-0223 ER -
@article{ author = "Prokopijević, Miloš and Simonović Radosavljević, Jasna and Spasojević, Dragica and Vojisavljević, Katarina and Radotić, Ksenija and Mitrović, Aleksandra Lj.", year = "2022", abstract = "Leaning stems of woody plants form reaction wood, in hardwood trees termed tension wood (TW). Typical TW fibers, gelatinous fibers (G-fibers), are characterized by an inner gelatinous cell wall layer (G-layer). Xyloglucan endotransglycosylases (XETs) was proposed as the essential enzyme in cell wall modifications in TW, by making xyloglucan (XG) cross-links between G- and S2-layers in G-fibers, and thus maintaining their contact. The etermination of TW presence in a sample is of great importance for the forest products industry, biofuel production, and tree physiology studies. However, TW is not easy to detect visually. The colorimetric assay for XET activity determination as an indicator of TW presence in a sample was tested on powdered stem segments of juvenile Populus x euramericana trees exposed to severe long-term static bending. In parallel, histochemical and ultrastructural characterization of stem samples of bent and control trees was performed. The tested colorimetric assay for XET activity determination could be suggested as a useful and easily applicable tool for fast screening of powdered wood samples for the presence of TW.", publisher = "De Gruyter", journal = "Holzforschung", title = "XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending", pages = "2505-2500", number = "6", volume = "76", doi = "10.1515/hf-2021-0223" }
Prokopijević, M., Simonović Radosavljević, J., Spasojević, D., Vojisavljević, K., Radotić, K.,& Mitrović, A. Lj.. (2022). XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending. in Holzforschung De Gruyter., 76(6), 2500-2505. https://doi.org/10.1515/hf-2021-0223
Prokopijević M, Simonović Radosavljević J, Spasojević D, Vojisavljević K, Radotić K, Mitrović AL. XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending. in Holzforschung. 2022;76(6):2500-2505. doi:10.1515/hf-2021-0223 .
Prokopijević, Miloš, Simonović Radosavljević, Jasna, Spasojević, Dragica, Vojisavljević, Katarina, Radotić, Ksenija, Mitrović, Aleksandra Lj., "XET activity determination in powdered wood samples as an indicator of tension wood, tested on juvenile Populus x euramericana exposed to severe long-term static bending" in Holzforschung, 76, no. 6 (2022):2500-2505, https://doi.org/10.1515/hf-2021-0223 . .