Germanium-68 as an Adequate Tracer for Silicon Transport in Plants. Characterization of Silicon Uptake in Different Crop Species
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2007
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A basic problem in silicon (Si) uptake studies in biology is the lack of an appropriate radioactive isotope. Radioactive germanium-68 (Ge-68) has been used previously as a Si tracer in biological materials, but its suitability for the study of Si transport in higher plants is still untested. In this study, we investigated Ge-68-traced Si uptake by four crop species differing widely in uptake capacity for Si, including rice (Oryza sativa), barley (Hordeum vulgare), cucumber (Cucumis sativus), and tomato (Lycopersicon esculentum). Maintenance of a Ge-68:Si molar ratio that was similar in the plant tissues of all four plant species to that supplied in the nutrient solution over a wide range of Si concentrations demonstrated the absence of discrimination between Ge-68 and Si. Further, using the Ge-68 tracer, a typical Michaelis-Menten uptake kinetics for Si was found in rice, barley, and cucumber. Compared to rice, the relative proportion of root-to-shoot translocated Si was lower in barle...y and cucumber and especially in tomato (only 30%). Uptake and translocation of Si in rice, barley, and cucumber (Si accumulators) were strongly inhibited by 2,4-dinitrophenol and HgCl2, but in tomato, as a Si-excluding species, both inhibitors produced the opposite effect. In conclusion, our results suggest the use of the Ge-68 tracer method as an appropriate choice for future studies of Si transport in plants. Our findings also indicate that the restriction of Si from symplast to apoplast in the cortex of Si excluders is a metabolically active process.
Ključne reči:
germanium / silicon / transport / rice / barley / cucumber / tomatoIzvor:
Plant Physiology, 2007, 143, 1, 495-503Izdavač:
- Amer Soc Plant Biologists, Rockville
DOI: 10.1104/pp.106.090845
ISSN: 0032-0889
PubMed: 17098850
WoS: 000243350600044
Scopus: 2-s2.0-33846338556
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Nikolic, Miroslav AU - Nikolić, Nina AU - Liang, Yongchao AU - Kirkby, Ernest A. AU - Romheld, V PY - 2007 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/176 AB - A basic problem in silicon (Si) uptake studies in biology is the lack of an appropriate radioactive isotope. Radioactive germanium-68 (Ge-68) has been used previously as a Si tracer in biological materials, but its suitability for the study of Si transport in higher plants is still untested. In this study, we investigated Ge-68-traced Si uptake by four crop species differing widely in uptake capacity for Si, including rice (Oryza sativa), barley (Hordeum vulgare), cucumber (Cucumis sativus), and tomato (Lycopersicon esculentum). Maintenance of a Ge-68:Si molar ratio that was similar in the plant tissues of all four plant species to that supplied in the nutrient solution over a wide range of Si concentrations demonstrated the absence of discrimination between Ge-68 and Si. Further, using the Ge-68 tracer, a typical Michaelis-Menten uptake kinetics for Si was found in rice, barley, and cucumber. Compared to rice, the relative proportion of root-to-shoot translocated Si was lower in barley and cucumber and especially in tomato (only 30%). Uptake and translocation of Si in rice, barley, and cucumber (Si accumulators) were strongly inhibited by 2,4-dinitrophenol and HgCl2, but in tomato, as a Si-excluding species, both inhibitors produced the opposite effect. In conclusion, our results suggest the use of the Ge-68 tracer method as an appropriate choice for future studies of Si transport in plants. Our findings also indicate that the restriction of Si from symplast to apoplast in the cortex of Si excluders is a metabolically active process. PB - Amer Soc Plant Biologists, Rockville T2 - Plant Physiology T1 - Germanium-68 as an Adequate Tracer for Silicon Transport in Plants. Characterization of Silicon Uptake in Different Crop Species EP - 503 IS - 1 SP - 495 VL - 143 DO - 10.1104/pp.106.090845 ER -
@article{ author = "Nikolic, Miroslav and Nikolić, Nina and Liang, Yongchao and Kirkby, Ernest A. and Romheld, V", year = "2007", abstract = "A basic problem in silicon (Si) uptake studies in biology is the lack of an appropriate radioactive isotope. Radioactive germanium-68 (Ge-68) has been used previously as a Si tracer in biological materials, but its suitability for the study of Si transport in higher plants is still untested. In this study, we investigated Ge-68-traced Si uptake by four crop species differing widely in uptake capacity for Si, including rice (Oryza sativa), barley (Hordeum vulgare), cucumber (Cucumis sativus), and tomato (Lycopersicon esculentum). Maintenance of a Ge-68:Si molar ratio that was similar in the plant tissues of all four plant species to that supplied in the nutrient solution over a wide range of Si concentrations demonstrated the absence of discrimination between Ge-68 and Si. Further, using the Ge-68 tracer, a typical Michaelis-Menten uptake kinetics for Si was found in rice, barley, and cucumber. Compared to rice, the relative proportion of root-to-shoot translocated Si was lower in barley and cucumber and especially in tomato (only 30%). Uptake and translocation of Si in rice, barley, and cucumber (Si accumulators) were strongly inhibited by 2,4-dinitrophenol and HgCl2, but in tomato, as a Si-excluding species, both inhibitors produced the opposite effect. In conclusion, our results suggest the use of the Ge-68 tracer method as an appropriate choice for future studies of Si transport in plants. Our findings also indicate that the restriction of Si from symplast to apoplast in the cortex of Si excluders is a metabolically active process.", publisher = "Amer Soc Plant Biologists, Rockville", journal = "Plant Physiology", title = "Germanium-68 as an Adequate Tracer for Silicon Transport in Plants. Characterization of Silicon Uptake in Different Crop Species", pages = "503-495", number = "1", volume = "143", doi = "10.1104/pp.106.090845" }
Nikolic, M., Nikolić, N., Liang, Y., Kirkby, E. A.,& Romheld, V.. (2007). Germanium-68 as an Adequate Tracer for Silicon Transport in Plants. Characterization of Silicon Uptake in Different Crop Species. in Plant Physiology Amer Soc Plant Biologists, Rockville., 143(1), 495-503. https://doi.org/10.1104/pp.106.090845
Nikolic M, Nikolić N, Liang Y, Kirkby EA, Romheld V. Germanium-68 as an Adequate Tracer for Silicon Transport in Plants. Characterization of Silicon Uptake in Different Crop Species. in Plant Physiology. 2007;143(1):495-503. doi:10.1104/pp.106.090845 .
Nikolic, Miroslav, Nikolić, Nina, Liang, Yongchao, Kirkby, Ernest A., Romheld, V, "Germanium-68 as an Adequate Tracer for Silicon Transport in Plants. Characterization of Silicon Uptake in Different Crop Species" in Plant Physiology, 143, no. 1 (2007):495-503, https://doi.org/10.1104/pp.106.090845 . .