PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP
Authors
Spasojević, DragicaProkopijević, Miloš
Prodanović, Olivera
Pantić, Nevena
Stanković, Mira
Radotić, Ksenija
Prodanović, Radivoje
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Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it
has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties,
such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate
was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within
hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system
generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small
amount of leaking enzyme shows potential for longer storage and reuse.
Keywords:
alginate / horseradish peroxidase / chemical modification / immobilizationSource:
29th International Conference Ecological Truth and Environmental Research - EcoTER'22, 2022, 360-363Publisher:
- University of Belgrade, Technical Faculty in Bor
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - CONF AU - Spasojević, Dragica AU - Prokopijević, Miloš AU - Prodanović, Olivera AU - Pantić, Nevena AU - Stanković, Mira AU - Radotić, Ksenija AU - Prodanović, Radivoje PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1767 AB - Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties, such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small amount of leaking enzyme shows potential for longer storage and reuse. PB - University of Belgrade, Technical Faculty in Bor C3 - 29th International Conference Ecological Truth and Environmental Research - EcoTER'22 T1 - PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP EP - 363 SP - 360 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1767 ER -
@conference{ author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Pantić, Nevena and Stanković, Mira and Radotić, Ksenija and Prodanović, Radivoje", year = "2022", abstract = "Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties, such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small amount of leaking enzyme shows potential for longer storage and reuse.", publisher = "University of Belgrade, Technical Faculty in Bor", journal = "29th International Conference Ecological Truth and Environmental Research - EcoTER'22", title = "PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP", pages = "363-360", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1767" }
Spasojević, D., Prokopijević, M., Prodanović, O., Pantić, N., Stanković, M., Radotić, K.,& Prodanović, R.. (2022). PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP. in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22 University of Belgrade, Technical Faculty in Bor., 360-363. https://hdl.handle.net/21.15107/rcub_rimsi_1767
Spasojević D, Prokopijević M, Prodanović O, Pantić N, Stanković M, Radotić K, Prodanović R. PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP. in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22. 2022;:360-363. https://hdl.handle.net/21.15107/rcub_rimsi_1767 .
Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Pantić, Nevena, Stanković, Mira, Radotić, Ksenija, Prodanović, Radivoje, "PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP" in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22 (2022):360-363, https://hdl.handle.net/21.15107/rcub_rimsi_1767 .