Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies
2022
Аутори
Štrbac, FilipBosco, Antonio
Maurelli, Maria Paola
Ratajac, Radomir
Stojanović, Dragica
Simin, Nataša
Orcic, Dejan
Pusic, Ivan
Krnjajić, Slobodan
Sotiraki, Smaragda
Saralli, Giorgio
Cringoli, Giuseppe
Rinaldi, Laura
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of Origanum vulgare, Foeniculum vulgare, Satureja montana, Satureja hortensis and two types of Thymus vulgaris were the most effective. The dominant compounds of these EOs we...re carvacrol, thymol, anethol, p-cymene and gamma-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both T. vulgaris EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed.
Кључне речи:
phytotherapy / in vivo test / in vitro test / gastrointestinal nematodes / essential oils / anthelmintic efficacyИзвор:
Veterinary Sciences, 2022, 9, 2Издавач:
- MDPI, Basel
Финансирање / пројекти:
- COST network (Networking tool) [ECOST-STSM-CA16230-201019-113354]
- CA16230]
DOI: 10.3390/vetsci9020093
ISSN: 2306-7381
PubMed: 35202346
WoS: 000761999200001
Scopus: 2-s2.0-85125147604
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Štrbac, Filip AU - Bosco, Antonio AU - Maurelli, Maria Paola AU - Ratajac, Radomir AU - Stojanović, Dragica AU - Simin, Nataša AU - Orcic, Dejan AU - Pusic, Ivan AU - Krnjajić, Slobodan AU - Sotiraki, Smaragda AU - Saralli, Giorgio AU - Cringoli, Giuseppe AU - Rinaldi, Laura PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1548 AB - Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of Origanum vulgare, Foeniculum vulgare, Satureja montana, Satureja hortensis and two types of Thymus vulgaris were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and gamma-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both T. vulgaris EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed. PB - MDPI, Basel T2 - Veterinary Sciences T1 - Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies IS - 2 VL - 9 DO - 10.3390/vetsci9020093 ER -
@article{ author = "Štrbac, Filip and Bosco, Antonio and Maurelli, Maria Paola and Ratajac, Radomir and Stojanović, Dragica and Simin, Nataša and Orcic, Dejan and Pusic, Ivan and Krnjajić, Slobodan and Sotiraki, Smaragda and Saralli, Giorgio and Cringoli, Giuseppe and Rinaldi, Laura", year = "2022", abstract = "Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of Origanum vulgare, Foeniculum vulgare, Satureja montana, Satureja hortensis and two types of Thymus vulgaris were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and gamma-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both T. vulgaris EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed.", publisher = "MDPI, Basel", journal = "Veterinary Sciences", title = "Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies", number = "2", volume = "9", doi = "10.3390/vetsci9020093" }
Štrbac, F., Bosco, A., Maurelli, M. P., Ratajac, R., Stojanović, D., Simin, N., Orcic, D., Pusic, I., Krnjajić, S., Sotiraki, S., Saralli, G., Cringoli, G.,& Rinaldi, L.. (2022). Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies. in Veterinary Sciences MDPI, Basel., 9(2). https://doi.org/10.3390/vetsci9020093
Štrbac F, Bosco A, Maurelli MP, Ratajac R, Stojanović D, Simin N, Orcic D, Pusic I, Krnjajić S, Sotiraki S, Saralli G, Cringoli G, Rinaldi L. Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies. in Veterinary Sciences. 2022;9(2). doi:10.3390/vetsci9020093 .
Štrbac, Filip, Bosco, Antonio, Maurelli, Maria Paola, Ratajac, Radomir, Stojanović, Dragica, Simin, Nataša, Orcic, Dejan, Pusic, Ivan, Krnjajić, Slobodan, Sotiraki, Smaragda, Saralli, Giorgio, Cringoli, Giuseppe, Rinaldi, Laura, "Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies" in Veterinary Sciences, 9, no. 2 (2022), https://doi.org/10.3390/vetsci9020093 . .