Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study
Authorized Users Only
2022
Authors
Bartolić, DraganaMojović, Miloš
Prokopijević, Miloš
Đikanović, Daniela
Kalauzi, Aleksandar
Mutavdžić, Dragosav
Baosic, Rada
Radotić, Ksenija
Article (Published version)
Metadata
Show full item recordAbstract
BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fracti...on, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity.
Keywords:
Zea mays L / seeds / organic free radicals / lignin / fluorescence spectroscopy / EPR spectroscopy / AFB(1)Source:
Journal of the Science of Food and Agriculture, 2022, 102, 6, 2500-2505Publisher:
- Wiley, Hoboken
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200168 (University of Belgrade, Faculty of Chemistry) (RS-MESTD-inst-2020-200168)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200146 (University of Belgrade, Faculty of Physical Chemistry) (RS-MESTD-inst-2020-200146)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200051 (Institute of General and Physical Chemistry, Belgrade) (RS-MESTD-inst-2020-200051)
DOI: 10.1002/jsfa.11591
ISSN: 0022-5142
PubMed: 34676551
WoS: 000716540500001
Scopus: 2-s2.0-85118771838
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Bartolić, Dragana AU - Mojović, Miloš AU - Prokopijević, Miloš AU - Đikanović, Daniela AU - Kalauzi, Aleksandar AU - Mutavdžić, Dragosav AU - Baosic, Rada AU - Radotić, Ksenija PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1544 AB - BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fraction, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity. PB - Wiley, Hoboken T2 - Journal of the Science of Food and Agriculture T1 - Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study EP - 2505 IS - 6 SP - 2500 VL - 102 DO - 10.1002/jsfa.11591 ER -
@article{ author = "Bartolić, Dragana and Mojović, Miloš and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Mutavdžić, Dragosav and Baosic, Rada and Radotić, Ksenija", year = "2022", abstract = "BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fraction, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity.", publisher = "Wiley, Hoboken", journal = "Journal of the Science of Food and Agriculture", title = "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study", pages = "2505-2500", number = "6", volume = "102", doi = "10.1002/jsfa.11591" }
Bartolić, D., Mojović, M., Prokopijević, M., Đikanović, D., Kalauzi, A., Mutavdžić, D., Baosic, R.,& Radotić, K.. (2022). Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture Wiley, Hoboken., 102(6), 2500-2505. https://doi.org/10.1002/jsfa.11591
Bartolić D, Mojović M, Prokopijević M, Đikanović D, Kalauzi A, Mutavdžić D, Baosic R, Radotić K. Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture. 2022;102(6):2500-2505. doi:10.1002/jsfa.11591 .
Bartolić, Dragana, Mojović, Miloš, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Mutavdžić, Dragosav, Baosic, Rada, Radotić, Ksenija, "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study" in Journal of the Science of Food and Agriculture, 102, no. 6 (2022):2500-2505, https://doi.org/10.1002/jsfa.11591 . .