Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification
Само за регистроване кориснике
2022
Аутори
Ilicic, RenataJelušić, Aleksandra
Marković, Sanja
Barac, Goran
Bagi, Ferenc
Popović, Tatjana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry frui...tlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.
Кључне речи:
recG helicase / Prunus avium / MLSA / identification / bacterial leaf spotИзвор:
Annals of Applied Biology, 2022, 180, 1, 140-150Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200010 (Институт за заштиту биља и животну средину, Београд) (RS-MESTD-inst-2020-200010)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200117 (Универзитет у Новом Саду, Пољопривредни факултет) (RS-MESTD-inst-2020-200117)
DOI: 10.1111/aab.12717
ISSN: 0003-4746
WoS: 000671883200001
Scopus: 2-s2.0-85109756587
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Ilicic, Renata AU - Jelušić, Aleksandra AU - Marković, Sanja AU - Barac, Goran AU - Bagi, Ferenc AU - Popović, Tatjana PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1531 AB - In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology. PB - Wiley, Hoboken T2 - Annals of Applied Biology T1 - Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification EP - 150 IS - 1 SP - 140 VL - 180 DO - 10.1111/aab.12717 ER -
@article{ author = "Ilicic, Renata and Jelušić, Aleksandra and Marković, Sanja and Barac, Goran and Bagi, Ferenc and Popović, Tatjana", year = "2022", abstract = "In May 2016, an unusual appearance of leaf spot (water-soaked, brown-purple, round to angular surrounded with yellow halos) was observed on the leaves of wild cherry specimens grown in Rimski Sancevi, Vojvodina (North Serbia). The causal pathogen was isolated from the wild cherry diseased leaves on Nutrient Agar supplemented with 5% sucrose and identified as Pseudomonas cerasi based on multilocus sequence analysis (MLSA). PCR amplification and sequencing of four housekeeping genes-gapA, gltA, rpoD and gyrB-showed 100% (gapA, gltA), 99.81% (rpoD) and 99.67% (gyrB) identity with P. cerasi type strain CFBP8305(T) (=58(T) = LMG28609(T)) and strain PL963 sequences from NCBI database. Pseudomonas cerasi isolates (coded as RE10-RE19) were LOPAT +- - - + (Pseudomonas Group Ia) and GATTa + - + -, produced fluorescent pigment, were able to utilise lactic and aspartic acid, but not tartaric acid. All isolates were pathogenic on wild cherry seedlings and leaves, immature sweet and sour cherry fruitlets, as well as on lilac leaves and green bean pods. This study also aimed to design new primers (recG-F/recG-R) for amplification of recG gene (encoding ATP-dependent DNA helicase RecG) known for its crucial role in DNA recombination and repair. Amplification of this gene enabled high identification ability of our wild cherry isolates as well as isolates belonging to the closely related stone fruit pathogenic Pseudomonas species. The obtained results highlight the potential of using recG gene for the specific detection and the identification of pathogenic Pseudomonas syringae complex. This research presents the first report of P. cerasi infecting wild cherry as well as first description of this bacterium in Serbia. Obtained results indicate the risk from further spread of this bacterium with the infected cherries rootstock, having a key role in its epidemiology.", publisher = "Wiley, Hoboken", journal = "Annals of Applied Biology", title = "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification", pages = "150-140", number = "1", volume = "180", doi = "10.1111/aab.12717" }
Ilicic, R., Jelušić, A., Marković, S., Barac, G., Bagi, F.,& Popović, T.. (2022). Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology Wiley, Hoboken., 180(1), 140-150. https://doi.org/10.1111/aab.12717
Ilicic R, Jelušić A, Marković S, Barac G, Bagi F, Popović T. Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification. in Annals of Applied Biology. 2022;180(1):140-150. doi:10.1111/aab.12717 .
Ilicic, Renata, Jelušić, Aleksandra, Marković, Sanja, Barac, Goran, Bagi, Ferenc, Popović, Tatjana, "Pseudomonas cerasi, the new wild cherry pathogen in Serbia and the potential use of recG helicase in bacterial identification" in Annals of Applied Biology, 180, no. 1 (2022):140-150, https://doi.org/10.1111/aab.12717 . .