Cell wall response to UV radiation in needles of Picea omorika
Samo za registrovane korisnike
2021
Autori
Mitrović, Aleksandra Lj.![](/themes/MirageRIMSI/images/orcid.png)
Simonović Radosavljević, Jasna
![](/themes/MirageRIMSI/images/orcid.png)
Prokopijević, Miloš
![](/themes/MirageRIMSI/images/orcid.png)
Spasojević, Dragica
![](/themes/MirageRIMSI/images/orcid.png)
Kovacević, Jovana
Prodanović, Olivera
![](/themes/MirageRIMSI/images/orcid.png)
Todorović, Bratislav
![](/themes/MirageRIMSI/images/orcid.png)
Matović, Branko
![](/themes/MirageRIMSI/images/orcid.png)
Stanković, Mira
![](/themes/MirageRIMSI/images/orcid.png)
Maksimović, Vuk
![](/themes/MirageRIMSI/images/orcid.png)
Mutavdžić, Dragosav
![](/themes/MirageRIMSI/images/orcid.png)
Skocic, Milos
Pesic, Mirjana
![](/themes/MirageRIMSI/images/orcid.png)
Prokic, Ljiljana
![](/themes/MirageRIMSI/images/orcid.png)
Radotić, Ksenija
![](/themes/MirageRIMSI/images/orcid.png)
Članak u časopisu (Objavljena verzija)
![](/themes/MirageRIMSI//images/creativecommons/arr.png)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day-UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C=C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV-B), and ionic POD and covalent PO (UV-C). A connection between a...ctivities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling.
Ključne reči:
UV radiation / Polyphenol oxidases / Picea omorika / Phenolics / Peroxidases / Cell wall polymersIzvor:
Plant Physiology and Biochemistry, 2021, 161, 176-190Izdavač:
- Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200053 (Univerzitet u Beogradu, Institut za multidisciplinarna istraživanja) (RS-MESTD-inst-2020-200053)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200116 (Univerzitet u Beogradu, Poljoprivredni fakultet) (RS-MESTD-inst-2020-200116)
DOI: 10.1016/j.plaphy.2021.02.007
ISSN: 0981-9428
PubMed: 33618201
WoS: 000637955100018
Scopus: 2-s2.0-85101082745
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Mitrović, Aleksandra Lj. AU - Simonović Radosavljević, Jasna AU - Prokopijević, Miloš AU - Spasojević, Dragica AU - Kovacević, Jovana AU - Prodanović, Olivera AU - Todorović, Bratislav AU - Matović, Branko AU - Stanković, Mira AU - Maksimović, Vuk AU - Mutavdžić, Dragosav AU - Skocic, Milos AU - Pesic, Mirjana AU - Prokic, Ljiljana AU - Radotić, Ksenija PY - 2021 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1449 AB - The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day-UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C=C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV-B), and ionic POD and covalent PO (UV-C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling. PB - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux T2 - Plant Physiology and Biochemistry T1 - Cell wall response to UV radiation in needles of Picea omorika EP - 190 SP - 176 VL - 161 DO - 10.1016/j.plaphy.2021.02.007 ER -
@article{ author = "Mitrović, Aleksandra Lj. and Simonović Radosavljević, Jasna and Prokopijević, Miloš and Spasojević, Dragica and Kovacević, Jovana and Prodanović, Olivera and Todorović, Bratislav and Matović, Branko and Stanković, Mira and Maksimović, Vuk and Mutavdžić, Dragosav and Skocic, Milos and Pesic, Mirjana and Prokic, Ljiljana and Radotić, Ksenija", year = "2021", abstract = "The UV-B represents the minor fraction of the solar spectrum, while UV-C is not contained in natural solar radiation, but both radiation types can cause damaging effects in plants. Cell walls (CWs) are one of the targets for external stressors. Juvenile P. omorika trees were treated either with 21 day-high doses UV-B or with 7 day-UV-C in open-top chambers. Using spectroscopic and biochemical techniques, it was shown that the response to UV radiation includes numerous modifications in needle CW structure: relative content of xylan, xyloglucan, lignin and cellulose decreased; cellulose crystallinity changed; yield of lignin monomers with stronger connection of C=C in side chain with the ring increased; re-distribution of inter- and intra-polymer H-bonds occurred. The recovery was mediated by an increase in the activities and changes in isoform profiles of CW bound covalent peroxidases (POD) and polyphenol oxidases (PO) (UV-B), and ionic POD and covalent PO (UV-C). A connection between activities of specific POD/PO isoforms and phenolic species (m- and p-coumaric acid, pinoresinol and cinnamic acid derivatives) was demonstrated, and supported by changes in the sRNA profile. In vivo fluorometry showed phenolics accumulation in needle epidermal CWs. These results imply transversal connections between polymers and changed mechanical properties of needle CW as a response to UV. The CW alterations enabled maintenance of physiological functions, as indicated by the preserved chlorophyll content and/or organization. The current study provides evidence that in conifers, needle CW response to both UV-B and UV-C includes biochemical modifications and structural remodeling.", publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux", journal = "Plant Physiology and Biochemistry", title = "Cell wall response to UV radiation in needles of Picea omorika", pages = "190-176", volume = "161", doi = "10.1016/j.plaphy.2021.02.007" }
Mitrović, A. Lj., Simonović Radosavljević, J., Prokopijević, M., Spasojević, D., Kovacević, J., Prodanović, O., Todorović, B., Matović, B., Stanković, M., Maksimović, V., Mutavdžić, D., Skocic, M., Pesic, M., Prokic, L.,& Radotić, K.. (2021). Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 161, 176-190. https://doi.org/10.1016/j.plaphy.2021.02.007
Mitrović AL, Simonović Radosavljević J, Prokopijević M, Spasojević D, Kovacević J, Prodanović O, Todorović B, Matović B, Stanković M, Maksimović V, Mutavdžić D, Skocic M, Pesic M, Prokic L, Radotić K. Cell wall response to UV radiation in needles of Picea omorika. in Plant Physiology and Biochemistry. 2021;161:176-190. doi:10.1016/j.plaphy.2021.02.007 .
Mitrović, Aleksandra Lj., Simonović Radosavljević, Jasna, Prokopijević, Miloš, Spasojević, Dragica, Kovacević, Jovana, Prodanović, Olivera, Todorović, Bratislav, Matović, Branko, Stanković, Mira, Maksimović, Vuk, Mutavdžić, Dragosav, Skocic, Milos, Pesic, Mirjana, Prokic, Ljiljana, Radotić, Ksenija, "Cell wall response to UV radiation in needles of Picea omorika" in Plant Physiology and Biochemistry, 161 (2021):176-190, https://doi.org/10.1016/j.plaphy.2021.02.007 . .