An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes
Апстракт
Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in... free radical studies of biological systems, with a number of advantages inherent to them.
Кључне речи:
superoxide / spin-trap / spin-probe / maize plasma membrane / hydroxyl radical / EPRИзвор:
Journal of the Serbian Chemical Society, 2005, 70, 2, 177-186Издавач:
- Srpsko hemijsko društvo, Beograd
DOI: 10.2298/JSC0502177M
ISSN: 0352-5139
WoS: 000228072300005
Scopus: 2-s2.0-25144441582
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Mojović, Miloš AU - Spasojević, Ivan AU - Vuletic, M AU - Vučinić, Željko AU - Bačić, Goran PY - 2005 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/142 AB - Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them. PB - Srpsko hemijsko društvo, Beograd T2 - Journal of the Serbian Chemical Society T1 - An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes EP - 186 IS - 2 SP - 177 VL - 70 DO - 10.2298/JSC0502177M ER -
@article{ author = "Mojović, Miloš and Spasojević, Ivan and Vuletic, M and Vučinić, Željko and Bačić, Goran", year = "2005", abstract = "Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.", publisher = "Srpsko hemijsko društvo, Beograd", journal = "Journal of the Serbian Chemical Society", title = "An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes", pages = "186-177", number = "2", volume = "70", doi = "10.2298/JSC0502177M" }
Mojović, M., Spasojević, I., Vuletic, M., Vučinić, Ž.,& Bačić, G.. (2005). An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes. in Journal of the Serbian Chemical Society Srpsko hemijsko društvo, Beograd., 70(2), 177-186. https://doi.org/10.2298/JSC0502177M
Mojović M, Spasojević I, Vuletic M, Vučinić Ž, Bačić G. An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes. in Journal of the Serbian Chemical Society. 2005;70(2):177-186. doi:10.2298/JSC0502177M .
Mojović, Miloš, Spasojević, Ivan, Vuletic, M, Vučinić, Željko, Bačić, Goran, "An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes" in Journal of the Serbian Chemical Society, 70, no. 2 (2005):177-186, https://doi.org/10.2298/JSC0502177M . .