Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents
2020
Autori
Milojkov, Dusan V.Silvestre, Oscar F.
Stanic, Vojislav
Janjic, Goran V.
Mutavdžić, Dragosav
Milanović, Marija
Nieder, Jana B.
Članak u časopisu (Recenzirana verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiples states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Addit...ionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (P-3(0)-> H-3(5) transition of Pr3+ ion) and orange emission at 600 nm (D-1(2)-> H-3(4)), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.
Ključne reči:
Praseodymium / Fluorapatite nanocrystals / Cancer cells / BioimagingIzvor:
Journal of Luminescence, 2020, 217Izdavač:
- Amsterdam : Elsevier
Finansiranje / projekti:
- European Cooperation in Science and Technology through COST Action [MP1302]
- CCDR-N [NORTE-01-0145-FEDER-000019]
- Marie Curie fellowship, EU-EC, COFUND programme "NanoTRAINforGrowth" [600375]
- Nove tehnologije za monitoring i zaštitu životnog okruženja od štetnih hemijskih supstanci i radijacionog opterećenja (RS-43009)
- Sinteza nanoprahova i procesiranje keramike i nanokompozita sa specifičnim električnim i magnetnim svojstvima za primenu u integrisanim pasivnim komponentama (RS-45021)
- Istraživanja interakcija enzima sa toksičnim i farmakološki aktivnim molekulima (RS-172023)
- Ispitivanja odnosa struktura-funkcija u ćelijskom zidu biljaka i izmene strukture zida enzimskim inženjeringom (RS-173017)
Napomena:
- This is the peer-reviewed version of Milojkov, D. V., Silvestre, O. F., Stanic, V., Janjic, G. V., Mutavdžić, D., Milanović, M.,& Nieder, J. B.. (2020). Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence Amsterdam : Elsevier., 217. https://doi.org/10.1016/j.jlumin.2019.116757 conv_1225
DOI: 10.1016/j.jlumin.2019.116757
ISSN: 0022-2313
WoS: 000501362400035
Scopus: 2-s2.0-85073162138
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Milojkov, Dusan V. AU - Silvestre, Oscar F. AU - Stanic, Vojislav AU - Janjic, Goran V. AU - Mutavdžić, Dragosav AU - Milanović, Marija AU - Nieder, Jana B. PY - 2020 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1387 AB - Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiples states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (P-3(0)-> H-3(5) transition of Pr3+ ion) and orange emission at 600 nm (D-1(2)-> H-3(4)), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications. PB - Amsterdam : Elsevier T2 - Journal of Luminescence T1 - Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents VL - 217 DO - 10.1016/j.jlumin.2019.116757 ER -
@article{ author = "Milojkov, Dusan V. and Silvestre, Oscar F. and Stanic, Vojislav and Janjic, Goran V. and Mutavdžić, Dragosav and Milanović, Marija and Nieder, Jana B.", year = "2020", abstract = "Fluorapatite doped with rare-earth elements has a wide-range of biomedical applications. Here, a new type of fluorapatite nanocrystals doped with praseodymium (FAP-Pr) with excitation-emission profiles in visible part of the spectrum is fabricated. Energy levels of Pr3+ activator ion contain metastable multiples states that offer the possibility of efficient multicolor emission lines in FAP nanocrystals. Three types of FAP-Pr nanocrystals with 0.1%, 0.5% and 1% atomic percent of Pr3+ (along with the undoped FAP control sample) are studied. Their novel chemical production method is described, the FAP-Pr nanocrystals structure, biocompatibility and the suitability for cell imaging are analyzed. Physicochemical characterization confirms crystals down to nanometer size. In addition, quantum-chemical calculation predicts that Pr3+ ions are incorporated into the FAP crystal lattice at Ca2 (6 h) sites. In vitro viability results shows that FAP-Pr nanocrystals are nontoxic to live cells. Additionally, the cell uptake of the FAP-Pr nanocrystals is studied using fluorescence-based widefield and confocal microscopy. The nanocrystals show characteristic green emission at 545 nm (P-3(0)-> H-3(5) transition of Pr3+ ion) and orange emission at 600 nm (D-1(2)-> H-3(4)), which we use to discriminate from cell autofluorescence background. Orthogonal projections across 3D confocal stacks show that the nanocrystals are able to enter the cells positioning themselves within the cytoplasm. Overall, the new FAP-Pr nanocrystals are biocompatible and of the tested types, the 0.5% Pr3+ doped nanocrystals show the highest promise as a tracking nanoparticle probe for bioimaging applications.", publisher = "Amsterdam : Elsevier", journal = "Journal of Luminescence", title = "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents", volume = "217", doi = "10.1016/j.jlumin.2019.116757" }
Milojkov, D. V., Silvestre, O. F., Stanic, V., Janjic, G. V., Mutavdžić, D., Milanović, M.,& Nieder, J. B.. (2020). Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence Amsterdam : Elsevier., 217. https://doi.org/10.1016/j.jlumin.2019.116757
Milojkov DV, Silvestre OF, Stanic V, Janjic GV, Mutavdžić D, Milanović M, Nieder JB. Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents. in Journal of Luminescence. 2020;217. doi:10.1016/j.jlumin.2019.116757 .
Milojkov, Dusan V., Silvestre, Oscar F., Stanic, Vojislav, Janjic, Goran V., Mutavdžić, Dragosav, Milanović, Marija, Nieder, Jana B., "Fabrication and characterization of luminescent Pr3+ doped fluorapatite nanocrystals as bioimaging contrast agents" in Journal of Luminescence, 217 (2020), https://doi.org/10.1016/j.jlumin.2019.116757 . .