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Single yeast cell nanomotions correlate with cellular activity
dc.creator | Willaert, Ronnie | |
dc.creator | Vanden, Boer, Pieterjan | |
dc.creator | Malovichko, Anton | |
dc.creator | Alioscha-Perez, Mitchel | |
dc.creator | Radotić, Ksenija | |
dc.creator | Bartolić, Dragana | |
dc.creator | Kalauzi, Aleksandar | |
dc.creator | Villalba, Maria Ines | |
dc.creator | Sanglard, Dominique | |
dc.creator | Dietler, Giovanni | |
dc.creator | Sahli, Hichem | |
dc.creator | Kasas, Sandor | |
dc.date.accessioned | 2022-04-05T15:27:24Z | |
dc.date.available | 2022-04-05T15:27:24Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 2375-2548 | |
dc.identifier.uri | http://rimsi.imsi.bg.ac.rs/handle/123456789/1335 | |
dc.description.abstract | Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward. | en |
dc.publisher | Amer Assoc Advancement Science, Washington | |
dc.relation | Belgian Federal Science Policy Office (Belspo)Belgian Federal Science Policy Office | |
dc.relation | European Space Agency (ESA) PRODEX program (Yeast Bioreactor project) | |
dc.relation | Research Council of the Vrije Universiteit Brussel (Belgium) support the International Joint Research Group (IJRG) VUB-EPFL BioNanotechnology & NanoMedicine (NANO) and the Strategic Research Program [SRP11-M3D2] | |
dc.relation | Swiss National Grants [200021-144321, 407240-167137, CRSII5_173863] | |
dc.relation | Gebert Ruf Stiftung [GRS-024/14] | |
dc.relation | NASANational Aeronautics & Space Administration (NASA) [NNH16ZDA001N-CLDTCH] | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173017/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.source | Science Advances | |
dc.subject | yeast | |
dc.subject | nanomotion | |
dc.subject | optical microscopy | |
dc.subject | metabolic state | |
dc.subject | antifungals | |
dc.subject | temperature | |
dc.title | Single yeast cell nanomotions correlate with cellular activity | en |
dc.type | article | |
dc.rights.license | BY-NC | |
dc.citation.issue | 26 | |
dc.citation.other | 6(26): - | |
dc.citation.rank | aM21 | |
dc.citation.volume | 6 | |
dc.identifier.doi | 10.1126/sciadv.aba3139 | |
dc.identifier.fulltext | http://rimsi.imsi.bg.ac.rs/bitstream/id/272/1332.pdf | |
dc.identifier.pmid | 32637604 | |
dc.identifier.scopus | 2-s2.0-85087475349 | |
dc.identifier.wos | 000543504100018 | |
dc.type.version | publishedVersion |