Single yeast cell nanomotions correlate with cellular activity
2020
Authors
Willaert, RonnieVanden, Boer, Pieterjan
Malovichko, Anton
Alioscha-Perez, Mitchel
Radotić, Ksenija
Bartolić, Dragana
Kalauzi, Aleksandar
Villalba, Maria Ines
Sanglard, Dominique
Dietler, Giovanni
Sahli, Hichem
Kasas, Sandor
Article (Published version)
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Show full item recordAbstract
Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.
Keywords:
yeast / nanomotion / optical microscopy / metabolic state / antifungals / temperatureSource:
Science Advances, 2020, 6, 26Publisher:
- Amer Assoc Advancement Science, Washington
Funding / projects:
- Belgian Federal Science Policy Office (Belspo)Belgian Federal Science Policy Office
- European Space Agency (ESA) PRODEX program (Yeast Bioreactor project)
- Research Council of the Vrije Universiteit Brussel (Belgium) support the International Joint Research Group (IJRG) VUB-EPFL BioNanotechnology & NanoMedicine (NANO) and the Strategic Research Program [SRP11-M3D2]
- Swiss National Grants [200021-144321, 407240-167137, CRSII5_173863]
- Gebert Ruf Stiftung [GRS-024/14]
- NASANational Aeronautics & Space Administration (NASA) [NNH16ZDA001N-CLDTCH]
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-173017)
DOI: 10.1126/sciadv.aba3139
ISSN: 2375-2548
PubMed: 32637604
WoS: 000543504100018
Scopus: 2-s2.0-85087475349
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Willaert, Ronnie AU - Vanden, Boer, Pieterjan AU - Malovichko, Anton AU - Alioscha-Perez, Mitchel AU - Radotić, Ksenija AU - Bartolić, Dragana AU - Kalauzi, Aleksandar AU - Villalba, Maria Ines AU - Sanglard, Dominique AU - Dietler, Giovanni AU - Sahli, Hichem AU - Kasas, Sandor PY - 2020 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1335 AB - Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward. PB - Amer Assoc Advancement Science, Washington T2 - Science Advances T1 - Single yeast cell nanomotions correlate with cellular activity IS - 26 VL - 6 DO - 10.1126/sciadv.aba3139 ER -
@article{ author = "Willaert, Ronnie and Vanden, Boer, Pieterjan and Malovichko, Anton and Alioscha-Perez, Mitchel and Radotić, Ksenija and Bartolić, Dragana and Kalauzi, Aleksandar and Villalba, Maria Ines and Sanglard, Dominique and Dietler, Giovanni and Sahli, Hichem and Kasas, Sandor", year = "2020", abstract = "Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.", publisher = "Amer Assoc Advancement Science, Washington", journal = "Science Advances", title = "Single yeast cell nanomotions correlate with cellular activity", number = "26", volume = "6", doi = "10.1126/sciadv.aba3139" }
Willaert, R., Vanden, B. P., Malovichko, A., Alioscha-Perez, M., Radotić, K., Bartolić, D., Kalauzi, A., Villalba, M. I., Sanglard, D., Dietler, G., Sahli, H.,& Kasas, S.. (2020). Single yeast cell nanomotions correlate with cellular activity. in Science Advances Amer Assoc Advancement Science, Washington., 6(26). https://doi.org/10.1126/sciadv.aba3139
Willaert R, Vanden BP, Malovichko A, Alioscha-Perez M, Radotić K, Bartolić D, Kalauzi A, Villalba MI, Sanglard D, Dietler G, Sahli H, Kasas S. Single yeast cell nanomotions correlate with cellular activity. in Science Advances. 2020;6(26). doi:10.1126/sciadv.aba3139 .
Willaert, Ronnie, Vanden, Boer, Pieterjan, Malovichko, Anton, Alioscha-Perez, Mitchel, Radotić, Ksenija, Bartolić, Dragana, Kalauzi, Aleksandar, Villalba, Maria Ines, Sanglard, Dominique, Dietler, Giovanni, Sahli, Hichem, Kasas, Sandor, "Single yeast cell nanomotions correlate with cellular activity" in Science Advances, 6, no. 26 (2020), https://doi.org/10.1126/sciadv.aba3139 . .