Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis
Abstract
Net fluxes of H+, K+ and Ca2+ ions from maize (Zea mays L.) isolated leaf segments were measured non-invasively using ion-selective vibrating microelectrodes (the MIFE technique). Leaf segments were isolated from the blade base, containing actively elongating cells (basal segments), and from non-growing tip regions (tip segments). Ion fluxes were measured in response to bright white light (2600 mumol m(-2) s(-1)) from either the leaf segments or the underlying mesophyll (after stripping the epidermis). Fluxes measured from the mesophyll showed no significant difference between basal and tip regions. In leaf segments (epidermis attached), light-induced flux kinetics of all ions measured (H+, Ca2+ and K+) were strikingly different between the two regions. It appears that epidermal K+ fluxes are required to drive leaf expansion growth, whereas in the mesophyll light-induced K+ flux changes are likely to play a charge balancing role. Light-stimulated Ca2+ influx was not directly attributab...le either to leaf photosynthetic performance or to leaf expansion growth. It is concluded that light-induced ion flux changes are associated with both leaf growth and photosynthesis.
Keywords:
Zea mays L / potassium / photosynthesis / leaf / hydrogen / expansion growth / epidermis / calciumSource:
Plant Cell and Environment, 2005, 28, 3, 340-352Publisher:
- Wiley, Hoboken
DOI: 10.1111/j.1365-3040.2005.01270.x
ISSN: 0140-7791
PubMed: 16021786
WoS: 000227087600007
Scopus: 2-s2.0-14644425338
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Živanović, Branka D. AU - Pang, J AU - Shabala, Sergey PY - 2005 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/129 AB - Net fluxes of H+, K+ and Ca2+ ions from maize (Zea mays L.) isolated leaf segments were measured non-invasively using ion-selective vibrating microelectrodes (the MIFE technique). Leaf segments were isolated from the blade base, containing actively elongating cells (basal segments), and from non-growing tip regions (tip segments). Ion fluxes were measured in response to bright white light (2600 mumol m(-2) s(-1)) from either the leaf segments or the underlying mesophyll (after stripping the epidermis). Fluxes measured from the mesophyll showed no significant difference between basal and tip regions. In leaf segments (epidermis attached), light-induced flux kinetics of all ions measured (H+, Ca2+ and K+) were strikingly different between the two regions. It appears that epidermal K+ fluxes are required to drive leaf expansion growth, whereas in the mesophyll light-induced K+ flux changes are likely to play a charge balancing role. Light-stimulated Ca2+ influx was not directly attributable either to leaf photosynthetic performance or to leaf expansion growth. It is concluded that light-induced ion flux changes are associated with both leaf growth and photosynthesis. PB - Wiley, Hoboken T2 - Plant Cell and Environment T1 - Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis EP - 352 IS - 3 SP - 340 VL - 28 DO - 10.1111/j.1365-3040.2005.01270.x ER -
@article{ author = "Živanović, Branka D. and Pang, J and Shabala, Sergey", year = "2005", abstract = "Net fluxes of H+, K+ and Ca2+ ions from maize (Zea mays L.) isolated leaf segments were measured non-invasively using ion-selective vibrating microelectrodes (the MIFE technique). Leaf segments were isolated from the blade base, containing actively elongating cells (basal segments), and from non-growing tip regions (tip segments). Ion fluxes were measured in response to bright white light (2600 mumol m(-2) s(-1)) from either the leaf segments or the underlying mesophyll (after stripping the epidermis). Fluxes measured from the mesophyll showed no significant difference between basal and tip regions. In leaf segments (epidermis attached), light-induced flux kinetics of all ions measured (H+, Ca2+ and K+) were strikingly different between the two regions. It appears that epidermal K+ fluxes are required to drive leaf expansion growth, whereas in the mesophyll light-induced K+ flux changes are likely to play a charge balancing role. Light-stimulated Ca2+ influx was not directly attributable either to leaf photosynthetic performance or to leaf expansion growth. It is concluded that light-induced ion flux changes are associated with both leaf growth and photosynthesis.", publisher = "Wiley, Hoboken", journal = "Plant Cell and Environment", title = "Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis", pages = "352-340", number = "3", volume = "28", doi = "10.1111/j.1365-3040.2005.01270.x" }
Živanović, Branka D., Pang, J.,& Shabala, S.. (2005). Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis. in Plant Cell and Environment Wiley, Hoboken., 28(3), 340-352. https://doi.org/10.1111/j.1365-3040.2005.01270.x
Živanović, Branka D., Pang J, Shabala S. Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis. in Plant Cell and Environment. 2005;28(3):340-352. doi:10.1111/j.1365-3040.2005.01270.x .
Živanović, Branka D., Pang, J, Shabala, Sergey, "Light-induced transient ion flux responses from maize leaves and their association with leaf growth and photosynthesis" in Plant Cell and Environment, 28, no. 3 (2005):340-352, https://doi.org/10.1111/j.1365-3040.2005.01270.x . .