Приказ основних података о документу
Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation
dc.creator | Spasojević, Dragica | |
dc.creator | Prokopijević, Miloš | |
dc.creator | Prodanović, Olivera | |
dc.creator | Zelenović, Nevena | |
dc.creator | Polović, Natalija | |
dc.creator | Radotić, Ksenija | |
dc.creator | Prodanović, Radivoje | |
dc.date.accessioned | 2022-04-05T15:18:14Z | |
dc.date.available | 2022-04-05T15:18:14Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 1598-5032 | |
dc.identifier.uri | http://rimsi.imsi.bg.ac.rs/handle/123456789/1196 | |
dc.description.abstract | Derivatives of xylans were synthesized from corncob xylan by carboxymethylation, oxidization with different molar ratios of periodate (5, 10 15 and 20 mol%) and by reductive amination with tyramine. Modifications of tyramine carboxymethyl xylans (Tyr-CMX) were confirmed by FTIR, UV and NMR spectra. Concentration of ionizable groups increased from 1.5 mmol/g for carboxymethyl xylan (CMX) to 5.4 mmol/g for Tyr-CMX oxidized with 20 mol% of periodate. All Tyr-CMXs were able to form hydrogels the cross-linking reaction with horseradish peroxidase and peroxide. Tyr-CMXs were tested for amyloglucosidase (AG) encapsulation within hydrogel microbeads obtained in a reaction of emulsion polymerization with peroxidase. Average diameter of Tyr-CMX hydrogel microbeads was 52 +/- 25 mu m and after encapsulation optimization with respect to the extent of CMX modification with tyramine, the concentration of Tyr-CMX, and the amount of added AG, microbeads with AG specific activity of 2 U/mL and 20% yield of immobilization were obtained. The optimum pH of the immobilized AG was not changed compared to the soluble one, while half-life at 60 degrees C was increased around 10 times. The Michaelis-Menten constant for the immobilized enzyme, 1.03 mM, was significantly lower than that for the soluble one, 1.54 mM. After 5 cycles of repetitive use in batch reactor, the immobilized AG retained 68% of initial activity. | en |
dc.publisher | Polymer Soc Korea, Seoul | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173017/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS// | |
dc.rights | restrictedAccess | |
dc.source | Macromolecular Research | |
dc.subject | xylan | en |
dc.subject | tyramine | en |
dc.subject | polymerization | en |
dc.subject | peroxide | en |
dc.subject | hydrogel | en |
dc.subject | emulsion | en |
dc.title | Peroxidase-Sensitive Tyramine Carboxymethyl Xylan Hydrogels for Enzyme Encapsulation | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 771 | |
dc.citation.issue | 8 | |
dc.citation.other | 27(8): 764-771 | |
dc.citation.rank | M22 | |
dc.citation.spage | 764 | |
dc.citation.volume | 27 | |
dc.identifier.doi | 10.1007/s13233-019-7111-7 | |
dc.identifier.scopus | 2-s2.0-85065978831 | |
dc.identifier.wos | 000482406900005 | |
dc.type.version | publishedVersion |