Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi
Samo za registrovane korisnike
2018
Autori
Zeng, FanrongShabala, Sergey
Dragišić Maksimović, Jelena
Maksimović, Vuk
Bonales-Alatorre, Edgar
Shabala, Lana
Yu, Min
Zhang, Guoping
Živanović, Branka D.
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Efforts to breed salt tolerant crops could benefit from investigating previously unexplored traits. One of them is a tissue succulency. In this work, we have undertaken an electrophysiological and biochemical comparison of properties of mesophyll and storage parenchyma leaf tissues of a succulent halophyte species Carpobrotus rosii ("pigface"). We show that storage parenchyma cells of C. rossii act as Na+ sink and possessed both higher Na+ sequestration (298 vs. 215 mM NaCl in mesophyll) and better K+ retention ability. The latter traits was determined by the higher rate of H+-ATPase operation and higher nonenzymatic antioxidant activity in this tissue. Na+ uptake in both tissues was insensitive to either Gd3+ or elevated Ca2+ ruling out involvement of nonselective cation channels as a major path for Na+ entry. Patch-clamp experiments have revealed that Caprobrotus plants were capable to downregulate activity of fast vacuolar channels when exposed to saline environment; this ability wa...s higher in the storage parenchyma cells compared with mesophyll. Also, storage parenchyma cells have constitutively lower number of open slow vacuolar channels, whereas in mesophyll, this suppression was inducible by salt. Taken together, these results provide a mechanistic basis for efficient Na+ sequestration in the succulent leaf tissues.
Ključne reči:
vacuolar sodium sequestration / tonoplast channels / SV / storage parenchyma / reactive oxygen species / potassium retention / mesophyll / FV / antioxidant activityIzvor:
Plant Cell and Environment, 2018, 41, 11, 2654-2667Izdavač:
- Wiley, Hoboken
Finansiranje / projekti:
- Interakcije membrana sa unutarćelijskim i apoplastičnim prostorom: izučavanja bioenergetike i singalizacije koristeći biofizičke i biohemijske metode (RS-MESTD-Basic Research (BR or ON)-173040)
- Australian Research CouncilAustralian Research Council
DOI: 10.1111/pce.13391
ISSN: 0140-7791
PubMed: 29956332
WoS: 000447468100012
Scopus: 2-s2.0-85052396333
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Zeng, Fanrong AU - Shabala, Sergey AU - Dragišić Maksimović, Jelena AU - Maksimović, Vuk AU - Bonales-Alatorre, Edgar AU - Shabala, Lana AU - Yu, Min AU - Zhang, Guoping AU - Živanović, Branka D. PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1173 AB - Efforts to breed salt tolerant crops could benefit from investigating previously unexplored traits. One of them is a tissue succulency. In this work, we have undertaken an electrophysiological and biochemical comparison of properties of mesophyll and storage parenchyma leaf tissues of a succulent halophyte species Carpobrotus rosii ("pigface"). We show that storage parenchyma cells of C. rossii act as Na+ sink and possessed both higher Na+ sequestration (298 vs. 215 mM NaCl in mesophyll) and better K+ retention ability. The latter traits was determined by the higher rate of H+-ATPase operation and higher nonenzymatic antioxidant activity in this tissue. Na+ uptake in both tissues was insensitive to either Gd3+ or elevated Ca2+ ruling out involvement of nonselective cation channels as a major path for Na+ entry. Patch-clamp experiments have revealed that Caprobrotus plants were capable to downregulate activity of fast vacuolar channels when exposed to saline environment; this ability was higher in the storage parenchyma cells compared with mesophyll. Also, storage parenchyma cells have constitutively lower number of open slow vacuolar channels, whereas in mesophyll, this suppression was inducible by salt. Taken together, these results provide a mechanistic basis for efficient Na+ sequestration in the succulent leaf tissues. PB - Wiley, Hoboken T2 - Plant Cell and Environment T1 - Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi EP - 2667 IS - 11 SP - 2654 VL - 41 DO - 10.1111/pce.13391 ER -
@article{ author = "Zeng, Fanrong and Shabala, Sergey and Dragišić Maksimović, Jelena and Maksimović, Vuk and Bonales-Alatorre, Edgar and Shabala, Lana and Yu, Min and Zhang, Guoping and Živanović, Branka D.", year = "2018", abstract = "Efforts to breed salt tolerant crops could benefit from investigating previously unexplored traits. One of them is a tissue succulency. In this work, we have undertaken an electrophysiological and biochemical comparison of properties of mesophyll and storage parenchyma leaf tissues of a succulent halophyte species Carpobrotus rosii ("pigface"). We show that storage parenchyma cells of C. rossii act as Na+ sink and possessed both higher Na+ sequestration (298 vs. 215 mM NaCl in mesophyll) and better K+ retention ability. The latter traits was determined by the higher rate of H+-ATPase operation and higher nonenzymatic antioxidant activity in this tissue. Na+ uptake in both tissues was insensitive to either Gd3+ or elevated Ca2+ ruling out involvement of nonselective cation channels as a major path for Na+ entry. Patch-clamp experiments have revealed that Caprobrotus plants were capable to downregulate activity of fast vacuolar channels when exposed to saline environment; this ability was higher in the storage parenchyma cells compared with mesophyll. Also, storage parenchyma cells have constitutively lower number of open slow vacuolar channels, whereas in mesophyll, this suppression was inducible by salt. Taken together, these results provide a mechanistic basis for efficient Na+ sequestration in the succulent leaf tissues.", publisher = "Wiley, Hoboken", journal = "Plant Cell and Environment", title = "Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi", pages = "2667-2654", number = "11", volume = "41", doi = "10.1111/pce.13391" }
Zeng, F., Shabala, S., Dragišić Maksimović, J., Maksimović, V., Bonales-Alatorre, E., Shabala, L., Yu, M., Zhang, G.,& Živanović, Branka D.. (2018). Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi. in Plant Cell and Environment Wiley, Hoboken., 41(11), 2654-2667. https://doi.org/10.1111/pce.13391
Zeng F, Shabala S, Dragišić Maksimović J, Maksimović V, Bonales-Alatorre E, Shabala L, Yu M, Zhang G, Živanović, Branka D.. Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi. in Plant Cell and Environment. 2018;41(11):2654-2667. doi:10.1111/pce.13391 .
Zeng, Fanrong, Shabala, Sergey, Dragišić Maksimović, Jelena, Maksimović, Vuk, Bonales-Alatorre, Edgar, Shabala, Lana, Yu, Min, Zhang, Guoping, Živanović, Branka D., "Revealing mechanisms of salinity tissue tolerance in succulent halophytes: A case study for Carpobrotus rossi" in Plant Cell and Environment, 41, no. 11 (2018):2654-2667, https://doi.org/10.1111/pce.13391 . .