Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
Само за регистроване кориснике
2018
Аутори
Jovanović, JovanaKolarević, Stoimir
Milosković, Aleksandra
Radojković, Nataša
Simic, Vladica
Dojčinović, Biljana
Kracun-Kolarević, Margareta
Paunović, Momir
Kostić-Vuković, Jovana
Sunjog, Karolina
Timilijic, Jovana
Đorđević Aleksić, Jelena
Gačić, Zoran
Zegura, Bojana
Vukovic-Gacic, Branka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native... water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
Кључне речи:
The Velika Morava River / SOS/umuC / HepG2 cells / Eco/genotoxicity / Comet assay / Alburnus alburnusИзвор:
Science of the Total Environment, 2018, 621, 1289-1299Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Утицај квалитета компонената у исхрани ципринида на квалитет меса, губитке и економичност производње (RS-MESTD-Technological Development (TD or TR)-31011)
- Мерење и моделирање физичких, хемијских, биолошких и морфодинамичких параметара река и водних акумулација (RS-MESTD-Technological Development (TD or TR)-37009)
- Рибе као биоиндикатори стања квалитета отворених вода Србије (RS-MESTD-Basic Research (BR or ON)-173045)
DOI: 10.1016/j.scitotenv.2017.10.099
ISSN: 0048-9697
PubMed: 29056382
WoS: 000424196800127
Scopus: 2-s2.0-85031791196
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Jovanović, Jovana AU - Kolarević, Stoimir AU - Milosković, Aleksandra AU - Radojković, Nataša AU - Simic, Vladica AU - Dojčinović, Biljana AU - Kracun-Kolarević, Margareta AU - Paunović, Momir AU - Kostić-Vuković, Jovana AU - Sunjog, Karolina AU - Timilijic, Jovana AU - Đorđević Aleksić, Jelena AU - Gačić, Zoran AU - Zegura, Bojana AU - Vukovic-Gacic, Branka PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1163 AB - The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study. PB - Elsevier Science Bv, Amsterdam T2 - Science of the Total Environment T1 - Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ EP - 1299 SP - 1289 VL - 621 DO - 10.1016/j.scitotenv.2017.10.099 ER -
@article{ author = "Jovanović, Jovana and Kolarević, Stoimir and Milosković, Aleksandra and Radojković, Nataša and Simic, Vladica and Dojčinović, Biljana and Kracun-Kolarević, Margareta and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Timilijic, Jovana and Đorđević Aleksić, Jelena and Gačić, Zoran and Zegura, Bojana and Vukovic-Gacic, Branka", year = "2018", abstract = "The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Science of the Total Environment", title = "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ", pages = "1299-1289", volume = "621", doi = "10.1016/j.scitotenv.2017.10.099" }
Jovanović, J., Kolarević, S., Milosković, A., Radojković, N., Simic, V., Dojčinović, B., Kracun-Kolarević, M., Paunović, M., Kostić-Vuković, J., Sunjog, K., Timilijic, J., Đorđević Aleksić, J., Gačić, Z., Zegura, B.,& Vukovic-Gacic, B.. (2018). Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment Elsevier Science Bv, Amsterdam., 621, 1289-1299. https://doi.org/10.1016/j.scitotenv.2017.10.099
Jovanović J, Kolarević S, Milosković A, Radojković N, Simic V, Dojčinović B, Kracun-Kolarević M, Paunović M, Kostić-Vuković J, Sunjog K, Timilijic J, Đorđević Aleksić J, Gačić Z, Zegura B, Vukovic-Gacic B. Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment. 2018;621:1289-1299. doi:10.1016/j.scitotenv.2017.10.099 .
Jovanović, Jovana, Kolarević, Stoimir, Milosković, Aleksandra, Radojković, Nataša, Simic, Vladica, Dojčinović, Biljana, Kracun-Kolarević, Margareta, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Timilijic, Jovana, Đorđević Aleksić, Jelena, Gačić, Zoran, Zegura, Bojana, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ" in Science of the Total Environment, 621 (2018):1289-1299, https://doi.org/10.1016/j.scitotenv.2017.10.099 . .