The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study
Само за регистроване кориснике
2018
Аутори
Kolarević, StoimirSunjog, Karolina
Kracun-Kolarević, Margareta
Kostić-Vuković, Jovana
Jovanović, Jovana
Simonović, Predrag
Simic, Vladica
Piria, Marina
Gačić, Zoran
Lenhardt, Mirjana
Paunović, Momir
Vukovic-Gacic, Branka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the site...s with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] .
Кључне речи:
River pollution / RAPD fingerprints / In situ / Fish / DNA damageИзвор:
International Journal of Environmental Research, 2018, 12, 5, 703-712Издавач:
- Springer International Publishing Ag, Cham
Финансирање / пројекти:
- Managing the effects of multiple stressors on aquatic ecosystems under water scarcity (EU-FP7-603629)
- 170345
DOI: 10.1007/s41742-018-0127-6
ISSN: 1735-6865
WoS: 000443431600014
Scopus: 2-s2.0-85052658444
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Kolarević, Stoimir AU - Sunjog, Karolina AU - Kracun-Kolarević, Margareta AU - Kostić-Vuković, Jovana AU - Jovanović, Jovana AU - Simonović, Predrag AU - Simic, Vladica AU - Piria, Marina AU - Gačić, Zoran AU - Lenhardt, Mirjana AU - Paunović, Momir AU - Vukovic-Gacic, Branka PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1160 AB - The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] . PB - Springer International Publishing Ag, Cham T2 - International Journal of Environmental Research T1 - The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study EP - 712 IS - 5 SP - 703 VL - 12 DO - 10.1007/s41742-018-0127-6 ER -
@article{ author = "Kolarević, Stoimir and Sunjog, Karolina and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Jovanović, Jovana and Simonović, Predrag and Simic, Vladica and Piria, Marina and Gačić, Zoran and Lenhardt, Mirjana and Paunović, Momir and Vukovic-Gacic, Branka", year = "2018", abstract = "The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] .", publisher = "Springer International Publishing Ag, Cham", journal = "International Journal of Environmental Research", title = "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study", pages = "712-703", number = "5", volume = "12", doi = "10.1007/s41742-018-0127-6" }
Kolarević, S., Sunjog, K., Kracun-Kolarević, M., Kostić-Vuković, J., Jovanović, J., Simonović, P., Simic, V., Piria, M., Gačić, Z., Lenhardt, M., Paunović, M.,& Vukovic-Gacic, B.. (2018). The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research Springer International Publishing Ag, Cham., 12(5), 703-712. https://doi.org/10.1007/s41742-018-0127-6
Kolarević S, Sunjog K, Kracun-Kolarević M, Kostić-Vuković J, Jovanović J, Simonović P, Simic V, Piria M, Gačić Z, Lenhardt M, Paunović M, Vukovic-Gacic B. The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research. 2018;12(5):703-712. doi:10.1007/s41742-018-0127-6 .
Kolarević, Stoimir, Sunjog, Karolina, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Jovanović, Jovana, Simonović, Predrag, Simic, Vladica, Piria, Marina, Gačić, Zoran, Lenhardt, Mirjana, Paunović, Momir, Vukovic-Gacic, Branka, "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study" in International Journal of Environmental Research, 12, no. 5 (2018):703-712, https://doi.org/10.1007/s41742-018-0127-6 . .