Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization
Само за регистроване кориснике
2017
Аутори
Prokopijević, MilošProdanović, Olivera
Spasojević, Dragica
Kovacević, Gordana
Polović, Natalija
Radotić, Ksenija
Prodanović, Radivoje
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Pectin was modified by oxidation with sodium periodate at molar ratios of 2.5, 5, 10, 15 and 20 mol% and reductive amination with tyramine and sodium cyanoborohydride afterwards. Concentration of tyramine groups within modified pectin ranged from 54.5 to 538 mu mol/g of dry pectin while concentration of ionizable groups ranged from 3.0 to 4.0 mmol/g of dry polymer compared to 1.5 mmol/g before modification due to the introduction of amino group. All tyramine-pectins showed exceptional gelling properties and could form hydrogel both by cross-linking of carboxyl groups with calcium or by cross-linking phenol groups with peroxidase in the presence of hydrogen peroxide. These hydrogels were tested as carriers for soybean hull peroxidase (SHP) immobilization within microbeads formed in an emulsion based enzymatic polymerization reaction. SHP immobilized within tyramine-pectin microbeads had an increased thermal and organic solvent stability compared to the soluble enzyme. Immobilized SHP wa...s more active in acidic pH region and had slightly decreased K (m) value of 2.61 mM compared to the soluble enzyme. After 7 cycles of repeated use in batch reactor for pyrogallol oxidation microbeads, immobilized SHP retained half of the initial activity.
Кључне речи:
Soybean hull peroxidase / Pectin modification / Hydrogels / Enzyme immobilizationИзвор:
Applied Microbiology and Biotechnology, 2017, 101, 6, 2281-2290Издавач:
- Springer, New York
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-MESTD-Basic Research (BR or ON)-172049)
DOI: 10.1007/s00253-016-8002-x
ISSN: 0175-7598
PubMed: 27942755
WoS: 000395058800007
Scopus: 2-s2.0-85003848832
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Prokopijević, Miloš AU - Prodanović, Olivera AU - Spasojević, Dragica AU - Kovacević, Gordana AU - Polović, Natalija AU - Radotić, Ksenija AU - Prodanović, Radivoje PY - 2017 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1096 AB - Pectin was modified by oxidation with sodium periodate at molar ratios of 2.5, 5, 10, 15 and 20 mol% and reductive amination with tyramine and sodium cyanoborohydride afterwards. Concentration of tyramine groups within modified pectin ranged from 54.5 to 538 mu mol/g of dry pectin while concentration of ionizable groups ranged from 3.0 to 4.0 mmol/g of dry polymer compared to 1.5 mmol/g before modification due to the introduction of amino group. All tyramine-pectins showed exceptional gelling properties and could form hydrogel both by cross-linking of carboxyl groups with calcium or by cross-linking phenol groups with peroxidase in the presence of hydrogen peroxide. These hydrogels were tested as carriers for soybean hull peroxidase (SHP) immobilization within microbeads formed in an emulsion based enzymatic polymerization reaction. SHP immobilized within tyramine-pectin microbeads had an increased thermal and organic solvent stability compared to the soluble enzyme. Immobilized SHP was more active in acidic pH region and had slightly decreased K (m) value of 2.61 mM compared to the soluble enzyme. After 7 cycles of repeated use in batch reactor for pyrogallol oxidation microbeads, immobilized SHP retained half of the initial activity. PB - Springer, New York T2 - Applied Microbiology and Biotechnology T1 - Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization EP - 2290 IS - 6 SP - 2281 VL - 101 DO - 10.1007/s00253-016-8002-x ER -
@article{ author = "Prokopijević, Miloš and Prodanović, Olivera and Spasojević, Dragica and Kovacević, Gordana and Polović, Natalija and Radotić, Ksenija and Prodanović, Radivoje", year = "2017", abstract = "Pectin was modified by oxidation with sodium periodate at molar ratios of 2.5, 5, 10, 15 and 20 mol% and reductive amination with tyramine and sodium cyanoborohydride afterwards. Concentration of tyramine groups within modified pectin ranged from 54.5 to 538 mu mol/g of dry pectin while concentration of ionizable groups ranged from 3.0 to 4.0 mmol/g of dry polymer compared to 1.5 mmol/g before modification due to the introduction of amino group. All tyramine-pectins showed exceptional gelling properties and could form hydrogel both by cross-linking of carboxyl groups with calcium or by cross-linking phenol groups with peroxidase in the presence of hydrogen peroxide. These hydrogels were tested as carriers for soybean hull peroxidase (SHP) immobilization within microbeads formed in an emulsion based enzymatic polymerization reaction. SHP immobilized within tyramine-pectin microbeads had an increased thermal and organic solvent stability compared to the soluble enzyme. Immobilized SHP was more active in acidic pH region and had slightly decreased K (m) value of 2.61 mM compared to the soluble enzyme. After 7 cycles of repeated use in batch reactor for pyrogallol oxidation microbeads, immobilized SHP retained half of the initial activity.", publisher = "Springer, New York", journal = "Applied Microbiology and Biotechnology", title = "Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization", pages = "2290-2281", number = "6", volume = "101", doi = "10.1007/s00253-016-8002-x" }
Prokopijević, M., Prodanović, O., Spasojević, D., Kovacević, G., Polović, N., Radotić, K.,& Prodanović, R.. (2017). Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization. in Applied Microbiology and Biotechnology Springer, New York., 101(6), 2281-2290. https://doi.org/10.1007/s00253-016-8002-x
Prokopijević M, Prodanović O, Spasojević D, Kovacević G, Polović N, Radotić K, Prodanović R. Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization. in Applied Microbiology and Biotechnology. 2017;101(6):2281-2290. doi:10.1007/s00253-016-8002-x .
Prokopijević, Miloš, Prodanović, Olivera, Spasojević, Dragica, Kovacević, Gordana, Polović, Natalija, Radotić, Ksenija, Prodanović, Radivoje, "Tyramine-modified pectins via periodate oxidation for soybean hull peroxidase induced hydrogel formation and immobilization" in Applied Microbiology and Biotechnology, 101, no. 6 (2017):2281-2290, https://doi.org/10.1007/s00253-016-8002-x . .