Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine
Само за регистроване кориснике
2017
Аутори
Bozic, BojanaKorać Jačić, Jelena
Stanković, Dalibor M.
Stanić, Marina
Popovic-Bijelic, Ana
Bogdanović Pristov, Jelena
Spasojević, Ivan
Bajčetić, Milica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Toxic effects of unconjugated bilirubin (BR) in neonatal hyperbilirubinemia have been related to redox and/or coordinate interactions with Cu2+. However, the development and mechanisms of such interactions at physiological pH have not been resolved. This study shows that BR reduces Cu2+ to Cu1+ in 1:1 stoichiometry. Apparently, BR undergoes degradation, i.e. BR and Cu2+ do not form stable complexes. The binding of Cu2+ to inorganic phosphates, liposomal phosphate groups, or to chelating drug penicillamine, impedes redox interactions with BR. Cu1+ undergoes spontaneous oxidation by O-2 resulting in hydrogen peroxide accumulation and hydroxyl radical production. In relation to this, copper and BR induced synergistic oxidative/damaging effects on erythrocytes membrane, which were alleviated by penicillamine. The production of reactive oxygen species by BR and copper represents a plausible cause of BR toxic effects and cell damage in hyperbilirubinemia. Further examination of therapeutic p...otentials of copper chelators in the treatment of severe neonatal hyperbilirubinemia is needed.
Кључне речи:
Superoxide / Lipid peroxidation / Hyperbilirubinemia / Hydrogen peroxide / ErythrocytesИзвор:
Chemico-Biological Interactions, 2017, 278, 129-134Издавач:
- Elsevier Ireland Ltd, Clare
Финансирање / пројекти:
- Magbiovin project (FP7-ERA Chairs-Pilot Call) [621375]
- Молекуларни механизми редокс сигналинга у хомеостази, адаптацији и патологији (RS-MESTD-Basic Research (BR or ON)-173014)
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
DOI: 10.1016/j.cbi.2017.10.022
ISSN: 0009-2797
PubMed: 29079291
WoS: 000418302000016
Scopus: 2-s2.0-85032258652
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Bozic, Bojana AU - Korać Jačić, Jelena AU - Stanković, Dalibor M. AU - Stanić, Marina AU - Popovic-Bijelic, Ana AU - Bogdanović Pristov, Jelena AU - Spasojević, Ivan AU - Bajčetić, Milica PY - 2017 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1044 AB - Toxic effects of unconjugated bilirubin (BR) in neonatal hyperbilirubinemia have been related to redox and/or coordinate interactions with Cu2+. However, the development and mechanisms of such interactions at physiological pH have not been resolved. This study shows that BR reduces Cu2+ to Cu1+ in 1:1 stoichiometry. Apparently, BR undergoes degradation, i.e. BR and Cu2+ do not form stable complexes. The binding of Cu2+ to inorganic phosphates, liposomal phosphate groups, or to chelating drug penicillamine, impedes redox interactions with BR. Cu1+ undergoes spontaneous oxidation by O-2 resulting in hydrogen peroxide accumulation and hydroxyl radical production. In relation to this, copper and BR induced synergistic oxidative/damaging effects on erythrocytes membrane, which were alleviated by penicillamine. The production of reactive oxygen species by BR and copper represents a plausible cause of BR toxic effects and cell damage in hyperbilirubinemia. Further examination of therapeutic potentials of copper chelators in the treatment of severe neonatal hyperbilirubinemia is needed. PB - Elsevier Ireland Ltd, Clare T2 - Chemico-Biological Interactions T1 - Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine EP - 134 SP - 129 VL - 278 DO - 10.1016/j.cbi.2017.10.022 ER -
@article{ author = "Bozic, Bojana and Korać Jačić, Jelena and Stanković, Dalibor M. and Stanić, Marina and Popovic-Bijelic, Ana and Bogdanović Pristov, Jelena and Spasojević, Ivan and Bajčetić, Milica", year = "2017", abstract = "Toxic effects of unconjugated bilirubin (BR) in neonatal hyperbilirubinemia have been related to redox and/or coordinate interactions with Cu2+. However, the development and mechanisms of such interactions at physiological pH have not been resolved. This study shows that BR reduces Cu2+ to Cu1+ in 1:1 stoichiometry. Apparently, BR undergoes degradation, i.e. BR and Cu2+ do not form stable complexes. The binding of Cu2+ to inorganic phosphates, liposomal phosphate groups, or to chelating drug penicillamine, impedes redox interactions with BR. Cu1+ undergoes spontaneous oxidation by O-2 resulting in hydrogen peroxide accumulation and hydroxyl radical production. In relation to this, copper and BR induced synergistic oxidative/damaging effects on erythrocytes membrane, which were alleviated by penicillamine. The production of reactive oxygen species by BR and copper represents a plausible cause of BR toxic effects and cell damage in hyperbilirubinemia. Further examination of therapeutic potentials of copper chelators in the treatment of severe neonatal hyperbilirubinemia is needed.", publisher = "Elsevier Ireland Ltd, Clare", journal = "Chemico-Biological Interactions", title = "Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine", pages = "134-129", volume = "278", doi = "10.1016/j.cbi.2017.10.022" }
Bozic, B., Korać Jačić, J., Stanković, D. M., Stanić, M., Popovic-Bijelic, A., Bogdanović Pristov, J., Spasojević, I.,& Bajčetić, M.. (2017). Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine. in Chemico-Biological Interactions Elsevier Ireland Ltd, Clare., 278, 129-134. https://doi.org/10.1016/j.cbi.2017.10.022
Bozic B, Korać Jačić J, Stanković DM, Stanić M, Popovic-Bijelic A, Bogdanović Pristov J, Spasojević I, Bajčetić M. Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine. in Chemico-Biological Interactions. 2017;278:129-134. doi:10.1016/j.cbi.2017.10.022 .
Bozic, Bojana, Korać Jačić, Jelena, Stanković, Dalibor M., Stanić, Marina, Popovic-Bijelic, Ana, Bogdanović Pristov, Jelena, Spasojević, Ivan, Bajčetić, Milica, "Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine" in Chemico-Biological Interactions, 278 (2017):129-134, https://doi.org/10.1016/j.cbi.2017.10.022 . .