Mitochondrial activity detected by cantilever based sensor
2017
Autori
Stupar, PetarChomicki, Wojciech
Maillard, Caroline
Mikeladze, David
Kalauzi, Aleksandar
Radotić, Ksenija
Dietler, Giovanni
Kasas, Sandor
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Our team recently demonstrated that cantilever based devices can detect signature of life in a chemistry independent manner. In this technique, the organism of interest is attached onto a classical AFM cantilever. If alive, it induces nanometre scale oscillations of the cantilever that disappear once the organism is killed. The technique was successfully used on bacteria, yeast, vegetal and mammalian cells. In this work we demonstrate that the method can also be applied to sub-cellular organelles, such as mitochondria. Mitochondria are involved in cellular energy production and are present in most eukaryotic cells. Nowadays, it is believed that mitochondria were originally prokaryotes that colonized eukaryotic cells and that live in an endosymbiotic way ever since. Here we present that mitochondria are also animated by nanometre scale oscillations that depend on their metabolic state and that stop once they are inhibited. This observation opens novel avenues to investigate the numerous... mitochondria-related diseases in humans.
Ključne reči:
mitochondria / cantilever / nanomotion / atomic force / microscopy / metabolic stateIzvor:
Mechanical Sciences, 2017, 8, 1, 23-28Izdavač:
- Copernicus Gesellschaft Mbh, Gottingen
Finansiranje / projekti:
- Swiss National Grant [200021-144321]
- Gebert Ruf Stiftung and Centre Cooperation & Developpement Program [GRS 024/14]
- Ispitivanja odnosa struktura-funkcija u ćelijskom zidu biljaka i izmene strukture zida enzimskim inženjeringom (RS-MESTD-Basic Research (BR or ON)-173017)
- Sinteza, procesiranje i karakterizacija nanostrukturnih materijala za primenu u oblasti energije, mehaničkog inženjerstva, zaštite životne stredine i biomedicine (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45012)
DOI: 10.5194/ms-8-23-2017
ISSN: 2191-9151
WoS: 000397830100001
Scopus: 2-s2.0-85015409102
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Stupar, Petar AU - Chomicki, Wojciech AU - Maillard, Caroline AU - Mikeladze, David AU - Kalauzi, Aleksandar AU - Radotić, Ksenija AU - Dietler, Giovanni AU - Kasas, Sandor PY - 2017 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1037 AB - Our team recently demonstrated that cantilever based devices can detect signature of life in a chemistry independent manner. In this technique, the organism of interest is attached onto a classical AFM cantilever. If alive, it induces nanometre scale oscillations of the cantilever that disappear once the organism is killed. The technique was successfully used on bacteria, yeast, vegetal and mammalian cells. In this work we demonstrate that the method can also be applied to sub-cellular organelles, such as mitochondria. Mitochondria are involved in cellular energy production and are present in most eukaryotic cells. Nowadays, it is believed that mitochondria were originally prokaryotes that colonized eukaryotic cells and that live in an endosymbiotic way ever since. Here we present that mitochondria are also animated by nanometre scale oscillations that depend on their metabolic state and that stop once they are inhibited. This observation opens novel avenues to investigate the numerous mitochondria-related diseases in humans. PB - Copernicus Gesellschaft Mbh, Gottingen T2 - Mechanical Sciences T1 - Mitochondrial activity detected by cantilever based sensor EP - 28 IS - 1 SP - 23 VL - 8 DO - 10.5194/ms-8-23-2017 ER -
@article{ author = "Stupar, Petar and Chomicki, Wojciech and Maillard, Caroline and Mikeladze, David and Kalauzi, Aleksandar and Radotić, Ksenija and Dietler, Giovanni and Kasas, Sandor", year = "2017", abstract = "Our team recently demonstrated that cantilever based devices can detect signature of life in a chemistry independent manner. In this technique, the organism of interest is attached onto a classical AFM cantilever. If alive, it induces nanometre scale oscillations of the cantilever that disappear once the organism is killed. The technique was successfully used on bacteria, yeast, vegetal and mammalian cells. In this work we demonstrate that the method can also be applied to sub-cellular organelles, such as mitochondria. Mitochondria are involved in cellular energy production and are present in most eukaryotic cells. Nowadays, it is believed that mitochondria were originally prokaryotes that colonized eukaryotic cells and that live in an endosymbiotic way ever since. Here we present that mitochondria are also animated by nanometre scale oscillations that depend on their metabolic state and that stop once they are inhibited. This observation opens novel avenues to investigate the numerous mitochondria-related diseases in humans.", publisher = "Copernicus Gesellschaft Mbh, Gottingen", journal = "Mechanical Sciences", title = "Mitochondrial activity detected by cantilever based sensor", pages = "28-23", number = "1", volume = "8", doi = "10.5194/ms-8-23-2017" }
Stupar, P., Chomicki, W., Maillard, C., Mikeladze, D., Kalauzi, A., Radotić, K., Dietler, G.,& Kasas, S.. (2017). Mitochondrial activity detected by cantilever based sensor. in Mechanical Sciences Copernicus Gesellschaft Mbh, Gottingen., 8(1), 23-28. https://doi.org/10.5194/ms-8-23-2017
Stupar P, Chomicki W, Maillard C, Mikeladze D, Kalauzi A, Radotić K, Dietler G, Kasas S. Mitochondrial activity detected by cantilever based sensor. in Mechanical Sciences. 2017;8(1):23-28. doi:10.5194/ms-8-23-2017 .
Stupar, Petar, Chomicki, Wojciech, Maillard, Caroline, Mikeladze, David, Kalauzi, Aleksandar, Radotić, Ksenija, Dietler, Giovanni, Kasas, Sandor, "Mitochondrial activity detected by cantilever based sensor" in Mechanical Sciences, 8, no. 1 (2017):23-28, https://doi.org/10.5194/ms-8-23-2017 . .