Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus
Нема приказа
Аутори
Kolarević, StoimirGačić, Zoran
Kostić-Vuković, Jovana
Sunjog, Karolina
Kracun-Kolarević, Margareta
Paunović, Momir
Knezevic-Vukcević, Jelena
Vukovic-Gacic, Branka
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater.
Кључне речи:
Hemocytes / Genotoxicity / Emerging pollutants / Comet assay / BiomarkersИзвор:
Clean-Soil Air Water, 2016, 44, 11, 1471-1476Издавач:
- Wiley, Hoboken
Финансирање / пројекти:
- Fate and effects of cytostatic pharmaceuticals in the environment and the identification of biomarkers for and improved risk assessment on environmental exposure (EU-FP7-265264)
- Биолошки активни природни производи као потенцијални извори нових лекова и дијететских суплемената (RS-MESTD-Basic Research (BR or ON)-172058)
DOI: 10.1002/clen.201500482
ISSN: 1863-0650
WoS: 000390002300005
Scopus: 2-s2.0-84994236303
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Kolarević, Stoimir AU - Gačić, Zoran AU - Kostić-Vuković, Jovana AU - Sunjog, Karolina AU - Kracun-Kolarević, Margareta AU - Paunović, Momir AU - Knezevic-Vukcević, Jelena AU - Vukovic-Gacic, Branka PY - 2016 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1014 AB - The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater. PB - Wiley, Hoboken T2 - Clean-Soil Air Water T1 - Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus EP - 1476 IS - 11 SP - 1471 VL - 44 DO - 10.1002/clen.201500482 ER -
@article{ author = "Kolarević, Stoimir and Gačić, Zoran and Kostić-Vuković, Jovana and Sunjog, Karolina and Kracun-Kolarević, Margareta and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka", year = "2016", abstract = "The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater.", publisher = "Wiley, Hoboken", journal = "Clean-Soil Air Water", title = "Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus", pages = "1476-1471", number = "11", volume = "44", doi = "10.1002/clen.201500482" }
Kolarević, S., Gačić, Z., Kostić-Vuković, J., Sunjog, K., Kracun-Kolarević, M., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2016). Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus. in Clean-Soil Air Water Wiley, Hoboken., 44(11), 1471-1476. https://doi.org/10.1002/clen.201500482
Kolarević S, Gačić Z, Kostić-Vuković J, Sunjog K, Kracun-Kolarević M, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus. in Clean-Soil Air Water. 2016;44(11):1471-1476. doi:10.1002/clen.201500482 .
Kolarević, Stoimir, Gačić, Zoran, Kostić-Vuković, Jovana, Sunjog, Karolina, Kracun-Kolarević, Margareta, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus" in Clean-Soil Air Water, 44, no. 11 (2016):1471-1476, https://doi.org/10.1002/clen.201500482 . .