Biological activities of phenolic compounds and ethanolic extract of Halacsya sendtneri (Boiss) Dorfler

Abstract

The objective of this study was to evaluate the efficacy of the ethanolic extract of endemic plant Halacsya sendtneri in inhibiting the growing of the test fungi and bacteria as well as to determine its genotoxic potential and toxicity using the Allium anaphase-telophase assay. Minimum inhibitory concentrations (MIC) were determined for 15 indicator strains of pathogens, representing both bacteria and fungi. The highest susceptibility to the ethanolic extract of H. sendtneri was exhibited by Pseudomonas glycinea (FSB4), (MIC=0.09 mg/ml) among the bacteria, and by Phialophora fastigiata (FSB81), (MIC=1.95 mg/ml) among the fungi. The composition of H. sendtneri extracts was also determined using HPLC analysis. Rosmarinic acid was found to be the dominant phenolic compound. The Allium anaphase-telophase genotoxicity assay revealed that the ethanolic extract of H. sendtneri at concentrations of 31.5 mg/l and below does not produce toxic or genotoxic effects. This is the first report of chemical constituents, genotoxic and antimicrobial activities of the endemic species, H. sendtneri.