Poređenje imobilizacije peroksidaze soje na različite glicidil metakrilat polimere

Abstract

Phenols are considered priority pollutants of wastewaters, they are non-biodegradable and the problem of their removal is a current and important enviromental issue. Enzymatic treatment of wastewaters using peroxidase and H2O2 is highly effective and selective method. Soybean hulls offer cheap source of large amounts of crude enzyme (soybean hull peroxidase - SHP), which can be used for this purpose. The aim of this research was to test different macroporous carriers for immobilization of soybean hull peroxidase. Our data demonstrate that SHP immobilization using glutaraldehyde activation is influenced by the pore size of the macroporous glycidyl methacrylate matrix. Both specific activity of the immobilized enzyme and immobilization yeald were increased with the increase in pore size, and the highest specific activity obtained was 22,8 U/g of carrier.