Poređenje imobilizacije peroksidaze soje na različite glicidil metakrilat polimere
Autori
Prokopijević, MilošProdanović, Olivera
Spasojević, Dragica
Stojanović, Željko
Radotić, Ksenija
Prodanović, Radivoje
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Phenols are considered priority pollutants of wastewaters, they are non-biodegradable and the problem of their
removal is a current and important enviromental issue. Enzymatic treatment of wastewaters using peroxidase
and H2O2 is highly effective and selective method. Soybean hulls offer cheap source of large amounts of crude
enzyme (soybean hull peroxidase - SHP), which can be used for this purpose. The aim of this research was to
test different macroporous carriers for immobilization of soybean hull peroxidase. Our data demonstrate that
SHP immobilization using glutaraldehyde activation is influenced by the pore size of the macroporous glycidyl
methacrylate matrix. Both specific activity of the immobilized enzyme and immobilization yeald were increased
with the increase in pore size, and the highest specific activity obtained was 22,8 U/g of carrier.
Ključne reči:
peroksidaza iz soje / glicidilmetakrilat / imobilizacijaIzvor:
50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012, 2012, 191-194Finansiranje / projekti:
- Alergeni, antitela, enzimi i mali fiziološki značajni molekuli: dizajn, struktura, funkcija i značaj (RS-MESTD-Basic Research (BR or ON)-172049)
- Ispitivanja odnosa struktura-funkcija u ćelijskom zidu biljaka i izmene strukture zida enzimskim inženjeringom (RS-MESTD-Basic Research (BR or ON)-173017)
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - CONF AU - Prokopijević, Miloš AU - Prodanović, Olivera AU - Spasojević, Dragica AU - Stojanović, Željko AU - Radotić, Ksenija AU - Prodanović, Radivoje PY - 2012 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/3122 AB - Phenols are considered priority pollutants of wastewaters, they are non-biodegradable and the problem of their removal is a current and important enviromental issue. Enzymatic treatment of wastewaters using peroxidase and H2O2 is highly effective and selective method. Soybean hulls offer cheap source of large amounts of crude enzyme (soybean hull peroxidase - SHP), which can be used for this purpose. The aim of this research was to test different macroporous carriers for immobilization of soybean hull peroxidase. Our data demonstrate that SHP immobilization using glutaraldehyde activation is influenced by the pore size of the macroporous glycidyl methacrylate matrix. Both specific activity of the immobilized enzyme and immobilization yeald were increased with the increase in pore size, and the highest specific activity obtained was 22,8 U/g of carrier. C3 - 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012 T1 - Poređenje imobilizacije peroksidaze soje na različite glicidil metakrilat polimere EP - 194 SP - 191 UR - https://hdl.handle.net/21.15107/rcub_rimsi_3122 ER -
@conference{ author = "Prokopijević, Miloš and Prodanović, Olivera and Spasojević, Dragica and Stojanović, Željko and Radotić, Ksenija and Prodanović, Radivoje", year = "2012", abstract = "Phenols are considered priority pollutants of wastewaters, they are non-biodegradable and the problem of their removal is a current and important enviromental issue. Enzymatic treatment of wastewaters using peroxidase and H2O2 is highly effective and selective method. Soybean hulls offer cheap source of large amounts of crude enzyme (soybean hull peroxidase - SHP), which can be used for this purpose. The aim of this research was to test different macroporous carriers for immobilization of soybean hull peroxidase. Our data demonstrate that SHP immobilization using glutaraldehyde activation is influenced by the pore size of the macroporous glycidyl methacrylate matrix. Both specific activity of the immobilized enzyme and immobilization yeald were increased with the increase in pore size, and the highest specific activity obtained was 22,8 U/g of carrier.", journal = "50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012", title = "Poređenje imobilizacije peroksidaze soje na različite glicidil metakrilat polimere", pages = "194-191", url = "https://hdl.handle.net/21.15107/rcub_rimsi_3122" }
Prokopijević, M., Prodanović, O., Spasojević, D., Stojanović, Ž., Radotić, K.,& Prodanović, R.. (2012). Poređenje imobilizacije peroksidaze soje na različite glicidil metakrilat polimere. in 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012, 191-194. https://hdl.handle.net/21.15107/rcub_rimsi_3122
Prokopijević M, Prodanović O, Spasojević D, Stojanović Ž, Radotić K, Prodanović R. Poređenje imobilizacije peroksidaze soje na različite glicidil metakrilat polimere. in 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012. 2012;:191-194. https://hdl.handle.net/21.15107/rcub_rimsi_3122 .
Prokopijević, Miloš, Prodanović, Olivera, Spasojević, Dragica, Stojanović, Željko, Radotić, Ksenija, Prodanović, Radivoje, "Poređenje imobilizacije peroksidaze soje na različite glicidil metakrilat polimere" in 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012 (2012):191-194, https://hdl.handle.net/21.15107/rcub_rimsi_3122 .