Albumin at the intersection between antioxidant and pro-oxidant in patients on peritoneal dialysis
Само за регистроване кориснике
2022
Аутори
Baralic, MarkoSpasojević, Ivan
Miljus, Goran
Šunderić, Miloš
Robajac, Dragana
Dobrijević, Zorana
Gligorijević, Nikola
Nedic, Olgica
Penezic, Ana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Albumin (HSA) is a multifunctional protein and due to its free Cys34 thiol group, represents a main source of free
thiols in the circulation. This property of HSA, combined with its ability to sequester redox active Cu(II) ions,
makes HSA a dominant circulatory antioxidant. End stage kidney disease (ESRD) is a condition accompanied by
elevated oxidative stress. The aim of the present study was to examine changes in the antioxidative capacity of
HSA and Cu(II) binding affinity in patients on peritoneal dialysis (PD), and relate it to the Cys34 thiol group
content and other structural changes of this molecule.
HSA molecules are modified in ESRD patients subjected to PD, having significantly lower thiol group and
bound Cu(II) content, reduced antioxidant capacity, an increased content of advanced glycation end-products
and altered conformation. Also, Cu(II) binding capacity of HSA in these patients is impaired, since a significant
portion of the high-affinity metal-binding site i...s unable to interact with Cu(II).
Taking into account that the concentration of Cu(II) in the circulation of ESRD patients is much higher than in
healthy persons and that Cu(II) binding capacity of HSA in these patients is significantly impaired, HSA may be
considered as a novel circulatory pro-oxidant, thus exacerbating oxidative stress.
Кључне речи:
End stage renal disease Peritoneal dialysis Albumin Copper Thiols Antioxidative capacityИзвор:
Free radical biology and medicine, 2022, 187, 105-112Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200019 (Универзитет у Београду, Институт за примену нуклеарне енергије - ИНЕП) (RS-MESTD-inst-2020-200019)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Baralic, Marko AU - Spasojević, Ivan AU - Miljus, Goran AU - Šunderić, Miloš AU - Robajac, Dragana AU - Dobrijević, Zorana AU - Gligorijević, Nikola AU - Nedic, Olgica AU - Penezic, Ana PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2985 AB - Albumin (HSA) is a multifunctional protein and due to its free Cys34 thiol group, represents a main source of free thiols in the circulation. This property of HSA, combined with its ability to sequester redox active Cu(II) ions, makes HSA a dominant circulatory antioxidant. End stage kidney disease (ESRD) is a condition accompanied by elevated oxidative stress. The aim of the present study was to examine changes in the antioxidative capacity of HSA and Cu(II) binding affinity in patients on peritoneal dialysis (PD), and relate it to the Cys34 thiol group content and other structural changes of this molecule. HSA molecules are modified in ESRD patients subjected to PD, having significantly lower thiol group and bound Cu(II) content, reduced antioxidant capacity, an increased content of advanced glycation end-products and altered conformation. Also, Cu(II) binding capacity of HSA in these patients is impaired, since a significant portion of the high-affinity metal-binding site is unable to interact with Cu(II). Taking into account that the concentration of Cu(II) in the circulation of ESRD patients is much higher than in healthy persons and that Cu(II) binding capacity of HSA in these patients is significantly impaired, HSA may be considered as a novel circulatory pro-oxidant, thus exacerbating oxidative stress. PB - Elsevier T2 - Free radical biology and medicine T1 - Albumin at the intersection between antioxidant and pro-oxidant in patients on peritoneal dialysis EP - 112 SP - 105 VL - 187 DO - 10.1016/j.freeradbiomed.2022.05.019 ER -
@article{ author = "Baralic, Marko and Spasojević, Ivan and Miljus, Goran and Šunderić, Miloš and Robajac, Dragana and Dobrijević, Zorana and Gligorijević, Nikola and Nedic, Olgica and Penezic, Ana", year = "2022", abstract = "Albumin (HSA) is a multifunctional protein and due to its free Cys34 thiol group, represents a main source of free thiols in the circulation. This property of HSA, combined with its ability to sequester redox active Cu(II) ions, makes HSA a dominant circulatory antioxidant. End stage kidney disease (ESRD) is a condition accompanied by elevated oxidative stress. The aim of the present study was to examine changes in the antioxidative capacity of HSA and Cu(II) binding affinity in patients on peritoneal dialysis (PD), and relate it to the Cys34 thiol group content and other structural changes of this molecule. HSA molecules are modified in ESRD patients subjected to PD, having significantly lower thiol group and bound Cu(II) content, reduced antioxidant capacity, an increased content of advanced glycation end-products and altered conformation. Also, Cu(II) binding capacity of HSA in these patients is impaired, since a significant portion of the high-affinity metal-binding site is unable to interact with Cu(II). Taking into account that the concentration of Cu(II) in the circulation of ESRD patients is much higher than in healthy persons and that Cu(II) binding capacity of HSA in these patients is significantly impaired, HSA may be considered as a novel circulatory pro-oxidant, thus exacerbating oxidative stress.", publisher = "Elsevier", journal = "Free radical biology and medicine", title = "Albumin at the intersection between antioxidant and pro-oxidant in patients on peritoneal dialysis", pages = "112-105", volume = "187", doi = "10.1016/j.freeradbiomed.2022.05.019" }
Baralic, M., Spasojević, I., Miljus, G., Šunderić, M., Robajac, D., Dobrijević, Z., Gligorijević, N., Nedic, O.,& Penezic, A.. (2022). Albumin at the intersection between antioxidant and pro-oxidant in patients on peritoneal dialysis. in Free radical biology and medicine Elsevier., 187, 105-112. https://doi.org/10.1016/j.freeradbiomed.2022.05.019
Baralic M, Spasojević I, Miljus G, Šunderić M, Robajac D, Dobrijević Z, Gligorijević N, Nedic O, Penezic A. Albumin at the intersection between antioxidant and pro-oxidant in patients on peritoneal dialysis. in Free radical biology and medicine. 2022;187:105-112. doi:10.1016/j.freeradbiomed.2022.05.019 .
Baralic, Marko, Spasojević, Ivan, Miljus, Goran, Šunderić, Miloš, Robajac, Dragana, Dobrijević, Zorana, Gligorijević, Nikola, Nedic, Olgica, Penezic, Ana, "Albumin at the intersection between antioxidant and pro-oxidant in patients on peritoneal dialysis" in Free radical biology and medicine, 187 (2022):105-112, https://doi.org/10.1016/j.freeradbiomed.2022.05.019 . .