Hormetic effects of low-dose radiation on lipid production in Chlorella sorokiniana
Аутори
Vojvodić, SnežanaStanić, Marina
Jevtović (born Romanović), Mima
Dimitrijević, Milena
Bogdanović Pristov, Jelena
Morina, Arian
Pittman, Jon
Spasojević, Ivan
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Radiation hormesis is a biological phenomenon whereby a relatively low doses of radiation
induce beneficial effects on biological parameters, such as viability, growth, and metabolic
profile 1. A number of studies have documented stimulatory effects of low-dose X- and
gamma radiation on plant growth 2. However, radiation hormesis has not been investigated
in microalgae which are becoming a highly relevant source of lipid-derived biofuels. The
aim of this study was to establish the effects of low-dose X-irradiation on biomass and
lipid production in Chlorella sorokiniana. The microalgae cultures were exposed to total
doses of 10, 20 or 50 Gy at different irradiation rates (0.565; 2.290 and 5.057 Gy/min).
Absorbed doses corresponded to ~10% of the total dose, as determined by dosimetry. Lipid
content was determined 30 days after irradiation using Nile Red fluorescence assay with
excitation/emission maxima at 530/570 nm (total lipids), 480/575 nm (neutral lipids) and
549/628 nm... (polar lipids) 3,4. Lipid content was normalized to dry biomass.
Кључне речи:
Chlorella irradiation lipidsИзвор:
Serbian Biochemical Society Eighth Conference, 2018, 193-195Финансирање / пројекти:
- the NATO Science for Peace and Security Project SPS G5320
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Vojvodić, Snežana AU - Stanić, Marina AU - Jevtović (born Romanović), Mima AU - Dimitrijević, Milena AU - Bogdanović Pristov, Jelena AU - Morina, Arian AU - Pittman, Jon AU - Spasojević, Ivan PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2962 AB - Radiation hormesis is a biological phenomenon whereby a relatively low doses of radiation induce beneficial effects on biological parameters, such as viability, growth, and metabolic profile 1. A number of studies have documented stimulatory effects of low-dose X- and gamma radiation on plant growth 2. However, radiation hormesis has not been investigated in microalgae which are becoming a highly relevant source of lipid-derived biofuels. The aim of this study was to establish the effects of low-dose X-irradiation on biomass and lipid production in Chlorella sorokiniana. The microalgae cultures were exposed to total doses of 10, 20 or 50 Gy at different irradiation rates (0.565; 2.290 and 5.057 Gy/min). Absorbed doses corresponded to ~10% of the total dose, as determined by dosimetry. Lipid content was determined 30 days after irradiation using Nile Red fluorescence assay with excitation/emission maxima at 530/570 nm (total lipids), 480/575 nm (neutral lipids) and 549/628 nm (polar lipids) 3,4. Lipid content was normalized to dry biomass. C3 - Serbian Biochemical Society Eighth Conference T1 - Hormetic effects of low-dose radiation on lipid production in Chlorella sorokiniana EP - 195 SP - 193 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2962 ER -
@conference{ author = "Vojvodić, Snežana and Stanić, Marina and Jevtović (born Romanović), Mima and Dimitrijević, Milena and Bogdanović Pristov, Jelena and Morina, Arian and Pittman, Jon and Spasojević, Ivan", year = "2018", abstract = "Radiation hormesis is a biological phenomenon whereby a relatively low doses of radiation induce beneficial effects on biological parameters, such as viability, growth, and metabolic profile 1. A number of studies have documented stimulatory effects of low-dose X- and gamma radiation on plant growth 2. However, radiation hormesis has not been investigated in microalgae which are becoming a highly relevant source of lipid-derived biofuels. The aim of this study was to establish the effects of low-dose X-irradiation on biomass and lipid production in Chlorella sorokiniana. The microalgae cultures were exposed to total doses of 10, 20 or 50 Gy at different irradiation rates (0.565; 2.290 and 5.057 Gy/min). Absorbed doses corresponded to ~10% of the total dose, as determined by dosimetry. Lipid content was determined 30 days after irradiation using Nile Red fluorescence assay with excitation/emission maxima at 530/570 nm (total lipids), 480/575 nm (neutral lipids) and 549/628 nm (polar lipids) 3,4. Lipid content was normalized to dry biomass.", journal = "Serbian Biochemical Society Eighth Conference", title = "Hormetic effects of low-dose radiation on lipid production in Chlorella sorokiniana", pages = "195-193", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2962" }
Vojvodić, S., Stanić, M., Jevtović (born Romanović), M., Dimitrijević, M., Bogdanović Pristov, J., Morina, A., Pittman, J.,& Spasojević, I.. (2018). Hormetic effects of low-dose radiation on lipid production in Chlorella sorokiniana. in Serbian Biochemical Society Eighth Conference, 193-195. https://hdl.handle.net/21.15107/rcub_rimsi_2962
Vojvodić S, Stanić M, Jevtović (born Romanović) M, Dimitrijević M, Bogdanović Pristov J, Morina A, Pittman J, Spasojević I. Hormetic effects of low-dose radiation on lipid production in Chlorella sorokiniana. in Serbian Biochemical Society Eighth Conference. 2018;:193-195. https://hdl.handle.net/21.15107/rcub_rimsi_2962 .
Vojvodić, Snežana, Stanić, Marina, Jevtović (born Romanović), Mima, Dimitrijević, Milena, Bogdanović Pristov, Jelena, Morina, Arian, Pittman, Jon, Spasojević, Ivan, "Hormetic effects of low-dose radiation on lipid production in Chlorella sorokiniana" in Serbian Biochemical Society Eighth Conference (2018):193-195, https://hdl.handle.net/21.15107/rcub_rimsi_2962 .