Lignin content in Picea omorika needles
2011
Аутори
Simonović Radosavljević, JasnaMitrović, Aleksandra Lj.
Đikanović, Daniela
Spasojević, Ivan
Mutavdžić, Dragosav
Radotić, Ksenija
Bogdanović Pristov, Jelena
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Picea omorika ((Pančić) Purkyně) is endemic to the Drina River valley in western Serbia and eastern Bosnia and Herzegovina near Višegrad. It was originally discovered near the village of Zaovine on the Tara Mountain in 1875, and named by the Serbian botanist Josif Pančić. Lignin in cell walls provides a mechanical support as well as a plant protection and resistance from the chemical and biological stress. In coniferous trees, coniferyl alcohol is a basic substrate for lignin production. In this study we analyzed lignin content in the needles of three half-sib P. omorika lines. The needles were obtained from 15-years old Picea omorika trees, grown in a generative seed orchard in Godovik (43°51' N, 20°02' E, 400 m a.s.l.), Serbia. Since there is no absolute reliable method for the determination of lignin content, lignin quantification was performed in three different ways: by acetyl bromide test using: 1) 2 mg of NaOH hydrolyzed cell walls or 2) 1 mg lignin-thioglycolic acid complex (LT...GA), as well as 3) by measuring absorbance of LTGA dissolved in 0.5 M NaOH at 280 nm. In acetyl bromide test, standard curves were obtained using freshly prepared solutions of coniferyl alcohol. In procedure (3), a standard curve was obtained with dehydrogenate polymer (DHP) synthesized from coniferyl alcohol.
The determination of lignin quantity in three different ways proved to be reasonable. Acetyl bromide test uses cell wall as a starting material, which is the main reason why lignin content might be overestimated. On the other hand, lignin quantification using thioglycolic acid may underestimate lignin content, since predominantly β-O-4 bonds are observed by this method. There was a significant difference in lignin concentration among all three lines. An acetyl bromide test of both isolated lignin and extract-free cell walls gave the same ratio of lignin concentration among the three lines. Lignin concentration was the highest in B5 line, according to both the acetyl bromide test and the maximum of lignin absorption in NaOH. As lignin make a part of plant antioxidant capacity, the results presented may indicate that among the studied P. omorika lines, B5 may have the highest protective capacity.
Кључне речи:
cell wall Picea omorika (Pančić) Purkynĕ ligninИзвор:
International conference Nature protection in XXI century, 2011, 411-414Издавач:
- Zavod za zaštitu prirode Crne Gore
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Simonović Radosavljević, Jasna AU - Mitrović, Aleksandra Lj. AU - Đikanović, Daniela AU - Spasojević, Ivan AU - Mutavdžić, Dragosav AU - Radotić, Ksenija AU - Bogdanović Pristov, Jelena PY - 2011 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2939 AB - Picea omorika ((Pančić) Purkyně) is endemic to the Drina River valley in western Serbia and eastern Bosnia and Herzegovina near Višegrad. It was originally discovered near the village of Zaovine on the Tara Mountain in 1875, and named by the Serbian botanist Josif Pančić. Lignin in cell walls provides a mechanical support as well as a plant protection and resistance from the chemical and biological stress. In coniferous trees, coniferyl alcohol is a basic substrate for lignin production. In this study we analyzed lignin content in the needles of three half-sib P. omorika lines. The needles were obtained from 15-years old Picea omorika trees, grown in a generative seed orchard in Godovik (43°51' N, 20°02' E, 400 m a.s.l.), Serbia. Since there is no absolute reliable method for the determination of lignin content, lignin quantification was performed in three different ways: by acetyl bromide test using: 1) 2 mg of NaOH hydrolyzed cell walls or 2) 1 mg lignin-thioglycolic acid complex (LTGA), as well as 3) by measuring absorbance of LTGA dissolved in 0.5 M NaOH at 280 nm. In acetyl bromide test, standard curves were obtained using freshly prepared solutions of coniferyl alcohol. In procedure (3), a standard curve was obtained with dehydrogenate polymer (DHP) synthesized from coniferyl alcohol. The determination of lignin quantity in three different ways proved to be reasonable. Acetyl bromide test uses cell wall as a starting material, which is the main reason why lignin content might be overestimated. On the other hand, lignin quantification using thioglycolic acid may underestimate lignin content, since predominantly β-O-4 bonds are observed by this method. There was a significant difference in lignin concentration among all three lines. An acetyl bromide test of both isolated lignin and extract-free cell walls gave the same ratio of lignin concentration among the three lines. Lignin concentration was the highest in B5 line, according to both the acetyl bromide test and the maximum of lignin absorption in NaOH. As lignin make a part of plant antioxidant capacity, the results presented may indicate that among the studied P. omorika lines, B5 may have the highest protective capacity. PB - Zavod za zaštitu prirode Crne Gore C3 - International conference Nature protection in XXI century T1 - Lignin content in Picea omorika needles EP - 414 SP - 411 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2939 ER -
@conference{ author = "Simonović Radosavljević, Jasna and Mitrović, Aleksandra Lj. and Đikanović, Daniela and Spasojević, Ivan and Mutavdžić, Dragosav and Radotić, Ksenija and Bogdanović Pristov, Jelena", year = "2011", abstract = "Picea omorika ((Pančić) Purkyně) is endemic to the Drina River valley in western Serbia and eastern Bosnia and Herzegovina near Višegrad. It was originally discovered near the village of Zaovine on the Tara Mountain in 1875, and named by the Serbian botanist Josif Pančić. Lignin in cell walls provides a mechanical support as well as a plant protection and resistance from the chemical and biological stress. In coniferous trees, coniferyl alcohol is a basic substrate for lignin production. In this study we analyzed lignin content in the needles of three half-sib P. omorika lines. The needles were obtained from 15-years old Picea omorika trees, grown in a generative seed orchard in Godovik (43°51' N, 20°02' E, 400 m a.s.l.), Serbia. Since there is no absolute reliable method for the determination of lignin content, lignin quantification was performed in three different ways: by acetyl bromide test using: 1) 2 mg of NaOH hydrolyzed cell walls or 2) 1 mg lignin-thioglycolic acid complex (LTGA), as well as 3) by measuring absorbance of LTGA dissolved in 0.5 M NaOH at 280 nm. In acetyl bromide test, standard curves were obtained using freshly prepared solutions of coniferyl alcohol. In procedure (3), a standard curve was obtained with dehydrogenate polymer (DHP) synthesized from coniferyl alcohol. The determination of lignin quantity in three different ways proved to be reasonable. Acetyl bromide test uses cell wall as a starting material, which is the main reason why lignin content might be overestimated. On the other hand, lignin quantification using thioglycolic acid may underestimate lignin content, since predominantly β-O-4 bonds are observed by this method. There was a significant difference in lignin concentration among all three lines. An acetyl bromide test of both isolated lignin and extract-free cell walls gave the same ratio of lignin concentration among the three lines. Lignin concentration was the highest in B5 line, according to both the acetyl bromide test and the maximum of lignin absorption in NaOH. As lignin make a part of plant antioxidant capacity, the results presented may indicate that among the studied P. omorika lines, B5 may have the highest protective capacity.", publisher = "Zavod za zaštitu prirode Crne Gore", journal = "International conference Nature protection in XXI century", title = "Lignin content in Picea omorika needles", pages = "414-411", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2939" }
Simonović Radosavljević, J., Mitrović, A. Lj., Đikanović, D., Spasojević, I., Mutavdžić, D., Radotić, K.,& Bogdanović Pristov, J.. (2011). Lignin content in Picea omorika needles. in International conference Nature protection in XXI century Zavod za zaštitu prirode Crne Gore., 411-414. https://hdl.handle.net/21.15107/rcub_rimsi_2939
Simonović Radosavljević J, Mitrović AL, Đikanović D, Spasojević I, Mutavdžić D, Radotić K, Bogdanović Pristov J. Lignin content in Picea omorika needles. in International conference Nature protection in XXI century. 2011;:411-414. https://hdl.handle.net/21.15107/rcub_rimsi_2939 .
Simonović Radosavljević, Jasna, Mitrović, Aleksandra Lj., Đikanović, Daniela, Spasojević, Ivan, Mutavdžić, Dragosav, Radotić, Ksenija, Bogdanović Pristov, Jelena, "Lignin content in Picea omorika needles" in International conference Nature protection in XXI century (2011):411-414, https://hdl.handle.net/21.15107/rcub_rimsi_2939 .