Application of differential pulse polarography in analysis of pectin

Abstract

Differential pulse polarography (DPP) with dropping mercury electrode (DME) was used to determine quantitatively galacturonic acid (GA), methylated polygalacturonic acid (met PGA), pectin (PC) and pectinase (PE) in 0.1 M NaClO4 as supporting electrolyte. Current peaks of GA, met PGA, PC and PE, at -1.55, - 1.50, -1.40 and -1.60 V vs SCE respectively, were found suitable for quantitative determination in the concentration range considered. Enzymatic reaction between PE and PC was followed and possibility to determine simultaneously of PC and PE was proved.