Hydroxyl radical generation and carbon centre depletion in the root cell wall isolate enriched with copper
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Copper is often accumulated in contaminated soils such as open cast mines, and is
toxic to plants. Effect of excess Cu (20 μM) on free radicals generation in the cell
wall was investigated in the root cell wall isolated from Verbascum thapsus L.
plants. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates
containing the spin-trapping reagent, 5-diethoxyphosphoryl-5-methyl-1-pyrroline-
N-oxide (DEPMPO), was used for detection and differentiation between free
radicals. EPR analysis showed an increase of DEPMPO/OH adduct concentration
induced by Cu accompanied by the decrease of concentration of carbon center
(DEPMPO/CH3) adduct originally determined in the cell wall of control plants.
This reaction was suppressed by boiling the cell wall with SDS to denaturate
proteins. We show that Cu induced •OH accumulation in the cell wall is enzyme
dependent.
Кључне речи:
Hydroxyl radical generation / copper / cell wall / Verbascum thapsus L.Извор:
11th international conference Physical Chemistry 2012, 2012, 400-402Издавач:
- Society of Physical Chemists of Serbia
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Morina, Filis AU - Milić Komić, Sonja AU - Mojovic, Milos AU - Veljović-Jovanović, Sonja PY - 2012 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2933 AB - Copper is often accumulated in contaminated soils such as open cast mines, and is toxic to plants. Effect of excess Cu (20 μM) on free radicals generation in the cell wall was investigated in the root cell wall isolated from Verbascum thapsus L. plants. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5-methyl-1-pyrroline- N-oxide (DEPMPO), was used for detection and differentiation between free radicals. EPR analysis showed an increase of DEPMPO/OH adduct concentration induced by Cu accompanied by the decrease of concentration of carbon center (DEPMPO/CH3) adduct originally determined in the cell wall of control plants. This reaction was suppressed by boiling the cell wall with SDS to denaturate proteins. We show that Cu induced •OH accumulation in the cell wall is enzyme dependent. PB - Society of Physical Chemists of Serbia C3 - 11th international conference Physical Chemistry 2012 T1 - Hydroxyl radical generation and carbon centre depletion in the root cell wall isolate enriched with copper EP - 402 SP - 400 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2933 ER -
@conference{ author = "Morina, Filis and Milić Komić, Sonja and Mojovic, Milos and Veljović-Jovanović, Sonja", year = "2012", abstract = "Copper is often accumulated in contaminated soils such as open cast mines, and is toxic to plants. Effect of excess Cu (20 μM) on free radicals generation in the cell wall was investigated in the root cell wall isolated from Verbascum thapsus L. plants. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5-methyl-1-pyrroline- N-oxide (DEPMPO), was used for detection and differentiation between free radicals. EPR analysis showed an increase of DEPMPO/OH adduct concentration induced by Cu accompanied by the decrease of concentration of carbon center (DEPMPO/CH3) adduct originally determined in the cell wall of control plants. This reaction was suppressed by boiling the cell wall with SDS to denaturate proteins. We show that Cu induced •OH accumulation in the cell wall is enzyme dependent.", publisher = "Society of Physical Chemists of Serbia", journal = "11th international conference Physical Chemistry 2012", title = "Hydroxyl radical generation and carbon centre depletion in the root cell wall isolate enriched with copper", pages = "402-400", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2933" }
Morina, F., Milić Komić, S., Mojovic, M.,& Veljović-Jovanović, S.. (2012). Hydroxyl radical generation and carbon centre depletion in the root cell wall isolate enriched with copper. in 11th international conference Physical Chemistry 2012 Society of Physical Chemists of Serbia., 400-402. https://hdl.handle.net/21.15107/rcub_rimsi_2933
Morina F, Milić Komić S, Mojovic M, Veljović-Jovanović S. Hydroxyl radical generation and carbon centre depletion in the root cell wall isolate enriched with copper. in 11th international conference Physical Chemistry 2012. 2012;:400-402. https://hdl.handle.net/21.15107/rcub_rimsi_2933 .
Morina, Filis, Milić Komić, Sonja, Mojovic, Milos, Veljović-Jovanović, Sonja, "Hydroxyl radical generation and carbon centre depletion in the root cell wall isolate enriched with copper" in 11th international conference Physical Chemistry 2012 (2012):400-402, https://hdl.handle.net/21.15107/rcub_rimsi_2933 .