The positive effect of naringenin on superficial membrane fluidity in human erythrocytes
Аутори
Ajdzanovic, VladimirSpasojević, Ivan
kontic ristic, Aleksandra
Milenkovic, Dragan
Živanović, Jasmina
jaric, ivana
Milosevic, Verica
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Superficial membrane fluidity is related to erythrocyte deformation behavior and affects the hemodynamics, especially in hypertension and atherosclerosis. Grapefruit flavanone naringenin, which is used as alternative therapeutic of cardiovascular conditions, can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of two nutritional doses of naringenin (lower - 0.1 µg/ml and higher - 1 µg/ml) on superficial membrane fluidity in human erythrocytes, which reflects their deformability. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 7-DS), the spectra of which are dependent on membrane fluidity. The results showed a significant (p=0.029) increase of membrane fluidity near the hydrophilic surface in erythrocytes treated with higher nutritional dose of naringenin, while the lower nutritional dose of naringenin was ineffective (p>0.05) in that membrane region. In the hydrophobic layer just below the ...erythrocyte membrane phospholipid heads both lower and higher dose of naringenin significantly (p=0.036 and p=0.028, respectively) increased membrane fluidity. These data document the positive and dose dependent effect of naringenin on superficial membrane fluidity in human erythrocytes, recommending its use in the cardiovascular conditions characterized by disturbed hemodynamics.
Кључне речи:
EPR fluidity erythrocytesИзвор:
Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia, 2014, 63-Издавач:
- Serbian Physiological Society
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Ajdzanovic, Vladimir AU - Spasojević, Ivan AU - kontic ristic, Aleksandra AU - Milenkovic, Dragan AU - Živanović, Jasmina AU - jaric, ivana AU - Milosevic, Verica PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2821 AB - Superficial membrane fluidity is related to erythrocyte deformation behavior and affects the hemodynamics, especially in hypertension and atherosclerosis. Grapefruit flavanone naringenin, which is used as alternative therapeutic of cardiovascular conditions, can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of two nutritional doses of naringenin (lower - 0.1 µg/ml and higher - 1 µg/ml) on superficial membrane fluidity in human erythrocytes, which reflects their deformability. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 7-DS), the spectra of which are dependent on membrane fluidity. The results showed a significant (p=0.029) increase of membrane fluidity near the hydrophilic surface in erythrocytes treated with higher nutritional dose of naringenin, while the lower nutritional dose of naringenin was ineffective (p>0.05) in that membrane region. In the hydrophobic layer just below the erythrocyte membrane phospholipid heads both lower and higher dose of naringenin significantly (p=0.036 and p=0.028, respectively) increased membrane fluidity. These data document the positive and dose dependent effect of naringenin on superficial membrane fluidity in human erythrocytes, recommending its use in the cardiovascular conditions characterized by disturbed hemodynamics. PB - Serbian Physiological Society C3 - Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia T1 - The positive effect of naringenin on superficial membrane fluidity in human erythrocytes SP - 63 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2821 ER -
@conference{ author = "Ajdzanovic, Vladimir and Spasojević, Ivan and kontic ristic, Aleksandra and Milenkovic, Dragan and Živanović, Jasmina and jaric, ivana and Milosevic, Verica", year = "2014", abstract = "Superficial membrane fluidity is related to erythrocyte deformation behavior and affects the hemodynamics, especially in hypertension and atherosclerosis. Grapefruit flavanone naringenin, which is used as alternative therapeutic of cardiovascular conditions, can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of two nutritional doses of naringenin (lower - 0.1 µg/ml and higher - 1 µg/ml) on superficial membrane fluidity in human erythrocytes, which reflects their deformability. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 7-DS), the spectra of which are dependent on membrane fluidity. The results showed a significant (p=0.029) increase of membrane fluidity near the hydrophilic surface in erythrocytes treated with higher nutritional dose of naringenin, while the lower nutritional dose of naringenin was ineffective (p>0.05) in that membrane region. In the hydrophobic layer just below the erythrocyte membrane phospholipid heads both lower and higher dose of naringenin significantly (p=0.036 and p=0.028, respectively) increased membrane fluidity. These data document the positive and dose dependent effect of naringenin on superficial membrane fluidity in human erythrocytes, recommending its use in the cardiovascular conditions characterized by disturbed hemodynamics.", publisher = "Serbian Physiological Society", journal = "Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia", title = "The positive effect of naringenin on superficial membrane fluidity in human erythrocytes", pages = "63", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2821" }
Ajdzanovic, V., Spasojević, I., kontic ristic, A., Milenkovic, D., Živanović, J., jaric, i.,& Milosevic, V.. (2014). The positive effect of naringenin on superficial membrane fluidity in human erythrocytes. in Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia Serbian Physiological Society., 63. https://hdl.handle.net/21.15107/rcub_rimsi_2821
Ajdzanovic V, Spasojević I, kontic ristic A, Milenkovic D, Živanović J, jaric I, Milosevic V. The positive effect of naringenin on superficial membrane fluidity in human erythrocytes. in Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia. 2014;:63. https://hdl.handle.net/21.15107/rcub_rimsi_2821 .
Ajdzanovic, Vladimir, Spasojević, Ivan, kontic ristic, Aleksandra, Milenkovic, Dragan, Živanović, Jasmina, jaric, ivana, Milosevic, Verica, "The positive effect of naringenin on superficial membrane fluidity in human erythrocytes" in Molecular, Cellular and Integrative Basis of Health and Disease: transdisciplinary approach: 3rd Congress of Physiological Sciences of Serbia with International Participation; 2014 Oct 29-3; Belgrade, Serbia (2014):63, https://hdl.handle.net/21.15107/rcub_rimsi_2821 .