Differentiation of wine commercial samples by using fluorescence spectroscopy and multivariate analysis

Abstract

Steady state fluorescence spectroscopy in combination with Multivariate Curve Resolution - Alternating Least Squares (MCR-ALS) for spectral analysis has been applied to differentiate commercial samples of wines available on the market. We have chosen the wine trade marks from the two Serbian producers that contain only one wine type, since such approach is a starting step for further analyses of the more complex samples containing different wine types. We also studied changes of the emission spectra of these samples during seven days after opening of the wine bottle. The emission spectra were recorded in the wavelength range 275 - 500 nm, after excitation in the range 255 - 300 nm. The spectra of the wine samples obtained from the same producer are very similar, i.e. contain the same components in the similar ratios. The changes of spectral components at 315 nm and 430 nm were the basis for differentiation of the wine samples from the two producers, as well as for estimation of the wine stability in time after the bottle opening. The results indicate that this may be a useful approach in fingerprinting wines from various producers, as well as in screening the stability of wine.